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  • Articles: DFG German National Licenses  (5)
  • Chloroplast  (3)
  • Biochemistry and Biotechnology  (2)
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  • Articles: DFG German National Licenses  (5)
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    The role of mitochondria in sulfolipid biosynthesis by Euglena chloroplasts
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 1001 (1989), S. 268-273 
    Details
    ISSN: 0005-2760
    Keywords: (Euglena) ; Chloroplast ; Cysteine sulfinic acid ; Mitochondrion ; S-Sulfocysteine ; Sulfite ; Sulfolipid biosynthesis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0005-2760(89)90110-0
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chlorophyll biosynthesis from glutamate or 5-aminolevulinate in intact Euglena chloroplasts (1985)
    Springer
    Planta 165 (1985), S. 12-22 
    Details
    ISSN: 1432-2048
    Keywords: 5-Aminolevulinic acid ; Chlorophyll synthesis ; Chloroplast ; Euglena ; Glutamic acid ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplasts observed, by electron microscopy, to be intact and uncontaminated, with high rates of light-dependent protein synthesis and CO2 fixation were isolated from cells grown on low-vitamin-B12 medium in the light or from cells grown in the same medium in the dark and then exposed to light for 36 h. Both types of chloroplasts were active but less variability was encountered with developing chloroplasts from 36-h cells. The 36-h chloroplasts showed good light-dependent incorporation of 5-amino-levulinic acid (ALA) or l-glutamic acid into chlorophyll (Chl) a which was linear for approx. 1 h. The specific activity of the Chl a remained the same after conversion to pheophytin a, methylpheophorbide a or pyromethylpheophorbide a and rechromatography, indicating that the label was in the tetrapyrrole. Incorporation of ALA was inhibited by levulinic acid, and by chloramphenicol and other inhibitors of translation of 70S-type chloroplast ribosomes at concentrations which did not appreciably inhibit photosynthesis but which blocked plastid protein synthesis nearly completely. Cycloheximide, an inhibitor of translation on 87S cytoplasmic ribosomes of Euglena, was without effect. The 70S inhibitors did not block uptake of labeled ALA. Although labeled glycine was taken up by the plastids, no incorporation into Chl a was observed. Thus the developing chloroplasts appear to contain all of the enzymatic machinery necessary to convert glutamic acid to Chl via the C5 pathway of ALA formation but the Shemin pathway from succinyl coenzyme A and glycine to ALA appears to be absent. The requirement for plastid protein synthesis concomitant with Chl synthesis indicates a regulatory interaction and also indicates that at least one protein influencing Chl synthesis is synthesized on 70S-type plastid ribosomes and is subject to metabolic turnover.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392206
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Comments on the translational and transcriptional origin of Euglena chloroplastic aminoacyl-tRNA synthetases (1976)
    Springer
    Archives of microbiology 109 (1976), S. 201-203 
    Details
    ISSN: 1432-072X
    Keywords: Euglena gracilis ; W3BUL ; Chloroplast ; Mitochondria ; Phenylalanyl-tRNA ; Synthetase ; Streptomycin ; Cycloheximide ; tRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A response to: “A consideration of Euglena gracilis W3BUL as a cytoplasmic control for the wild-type phenylalanyl-tRNA synthetase system” and “A reinvestigation of the sites of transcription and translation of Euglena chloroplastic phenylalanyl-tRNA synthetase” by J. L. Lesiewicz and D. S. Herson.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446628
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A simple method for accurately locating and cutting strips from the isoelectric focusing gel during two-dimensional electrophoresis using the phastsystem (1989)
    Weinheim : Wiley-Blackwell
    Electrophoresis 10 (1989), S. 223-224 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A rapid and simple method has been developed for precisely locating and cutting 1-3 mm strips from the isoelectric focusing gel during two-dimensional electrophoresis using the PhastSystem. The protocol utilizes an easily constructed styrofoam template with marks to guide accurate cutting of the strips from the sample lanes. This system greatly enhances the reproducibility and quality of the two-dimensional patterns obtained.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150100313
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrasensitive polychromatic silver staining of sodium dodecyl sulfate-polyacrylamide gels with the Gelcode system using the PhastSystem Development Unit (1989)
    Weinheim : Wiley-Blackwell
    Electrophoresis 10 (1989), S. 535-536 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The Gelcode color-based silver staining system, an improved formulation based on the original publication by Sammons et al. (Electrophoresis 1981, 2, 141-147) has been adapted to automated rapid staining in the PhastSystem Development Unit. The use of elevated temperatures in the fixation, washing, staining, and stabilization steps of the protocol reduces the total time of the process from 18 h to 1 h. The limit of detection, which is at least tenfold more sensitive than the silver staining protocol recommended for the PhastSystem, corresponds to 0.05-0.1 ng of protein per band. The method is applicable to both one- and two-dimensional polyacrylamide gels.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150100717
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    Paper (German National Licenses)
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