ZIB

1

Digitale Medien

Salivary insulin concentrations in Type 2 (non-insulin-dependent) diabetic patients and obese non-diabetic subjects: Relationship to changes in plasma insulin levels after an oral glucose load (1986)

Marchetti, P. ; Benzi, L. ; Masoni, A. ; [weitere]

Springer

Diabetologia 29 (1986), S. 695-698

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1432-0428

Schlagwort(e): Salivary insulin ; obese subjects ; Type 2 diabetic patients

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Summary The presence of immunoreactive insulin in saliva and its relationship to plasma immunoreactive insulin was investigated in healthy subjects, newly diagnosed non-obese Type 2 (non-insulin-dependent) diabetic patients and obese non-diabetic subjects, basally and after an oral glucose tolerance test. The mean ± SEM fasting values of plasma and salivary immunoreactive insulin were significantly higher in diabetic patients and obese non-diabetic subjects than in normal volunteers (p〈0.05). During the glucose challenge, the increase of salivary insulin was related with that of plasma in the three groups of subjects, with a time lag in normal and obese subjects. In normal volunteers, plasma and salivary peak values were respectively 49.5 ± 13.4 μU/ml (p〈0.05 vs obese subjects) at 60 min and 12.0±3.3μU/min (p〈0.05 vs obese subjects) at 120 min; in diabetic patients, the values were 51.7 ± 5.6 μU/ml (p〈0.05 vs obese subjects) and 14.6±4.1 μU/min at 120 min; in obese subjects, the peak value for plasma insulin was 111.5±40.1 μU/ml at 90 min and for salivary insulin 15.6 ± 5.1 μU/min at 120 min. A positive linear relationship was shown between plasma and salivary insulin during the oral glucose tolerance test. The identity of salivary insulin was assessed by reversed-phase HPLC. We conclude that salivary immunoreactive insulin can be found in Type 2 diabetic patients and in obese non-diabetic subjects, as well as normal volunteers, that plasma and salivary insulin are related after a glucose load, and that differences exist in salivary insulin secretion patterns among the three groups of subjects.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00870278

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

2

Digitale Medien

The direct effects of metformin on platelet function in vitro (1989)

Caterina, R. ; Marchetti, P. ; Bernini, W. ; [weitere]

Springer

European journal of clinical pharmacology 37 (1989), S. 211-213

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1432-1041

Schlagwort(e): metformin ; atherosclerosis ; platelets ; thromboxanes

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie , Medizin

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00558236

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

3

Digitale Medien

Lymphokine release during co-culture of human lympho-mononuclear cells and fresh or cultured human, porcine and bovine pancreatic islets (1996)

Giannarelli, R. ; Ferdeghini, M. ; Arvia, C. ; [weitere]

Springer

Acta diabetologica 33 (1996), S. 122-125

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1432-5233

Schlagwort(e): Key words Lymphokines ; Pancreatic islets

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract In this study we evaluated whether isolated human (HI), porcine (PI) and bovine (BI) islets, either fresh (Fr) or cultured for 4 weeks (4w) affect cytokine release from human lymphomononuclear cells (LMC) differently. We prepared LMC from peripheral blood by density gradient purification and co-cultured 1×106 LMC for 24 h with 100 hand-picked islets, either within 48 h of isolation or after culture for 4 weeks. Soluble interleukin-2 receptor (IL-2R), interferon-gamma (IFN), interleukin-4 (IL-4) and interleukin-10 (IL-10) were measured by sandwich enzyme-linked immunoadsorbent assay. Compared with controls (Ctrl, LMC without islets), Fr-HI, Fr-PI and Fr-BI caused a similar increase of IL-2R and IFN release, whereas 4w-HI and 4w-BI did not lead to any significant production of these two cytokines. IL-10 concentrations increased with Fr-PI and Fr-BI, but not with Fr-HI, and no major effect of the 4-week culture was seen. IL-4 levels were below the detection limit of the method used in these experiments. Thus, fresh allo- and xeno-islets caused a similar increase of the release of cytokines known to be markers of Th1 activation, whereas the release of IL-10, a marker of Th2 activation, increased with xeno-, but not with allo-islets; culturing the islets for 4 weeks decreased Th1, but not Th2 activation.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00569422

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

4

Digitale Medien

A prevalence study of known diabetes mellitus in Tuscany assessed from pharmaceutical prescriptions and other independent sources (1994)

Cianni, G. ; Benzi, L. ; Giannarelli, R. ; [weitere]

Springer

Acta diabetologica 31 (1994), S. 87-90

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1432-5233

Schlagwort(e): Diabetes mellitus ; Epidemiology ; Pharmaceutical prescriptions

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract This study evaluates the prevalence of diabetes mellitus (DM) in Pisa (Tuscany, Italy) using four independent data sources. The main source, represented by computerized prescriptions for anti-diabetic agents collected over a 4-month period, was validated using three secondary sources: (a) the list of diabetic patients who receive material of self-care from the National Health Service; (b) the clinical records of diabetic patients obtained from a random sample of family doctors; (c) the clinical records of diabetic patients attending our outpatient clinic. The main source provided 3806 patients, and 697 patients were added from the secondary sources, thus identifying a total number of 4503. The prevalence of known DM in the “Pisa area” exclusively reckoned by the main source, was 2.01%, and the prevalence corrected by the addition of the various sources resulted in 2.4%. The capture-recapture method showed a completeness of ascertainment of the survey of 90.1%, and thus an estimated prevalence of known diabetes of 2.64%. Of these, 141 patients had insulin-dependent diabetes mellitus (IDDM) corresponding to 3.2% of identified diabetic subjects (prevalence 0.07% inhabitants); 4362 patients had non-insulin-dependent diabetes mellitus (NIDDM), 96.8% of identified diabetic subjects (prevalence 2.36%). Of patients with NIDDM 10.5% was treated by diet, 65% with oral hypoglycaemic agents (OHA), 23% with insulin and 1.5% with insulin plus OHA. This study shows that the method used in this survey is suitable for epidemiological studies because it does not demand the cooperation of the diabetic patients, is addressed to the entire diabetic population without age discrimination and singles out the diabetic population in a very reliable way.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00570541

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

5

Digitale Medien

Effects of glibenclamide and metformin (alone or in combination) on insulin release from isolated human pancreatic islets (1997)

Lupi, R. ; Marchetti, P. ; Giannarelli, R. ; [weitere]

Springer

Acta diabetologica 34 (1997), S. 46-48

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1432-5233

Schlagwort(e): Key words Insulin release ; Human islets ; Glibenclamide ; Metformin

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract Isolated human pancreatic islets were prepared by collagenase digestion and density gradient purification, and the effects of glibenclamide (0.5 and 5.0 µmol/l) and metformin (20 and 200 µmol/l), alone or in combination, on insulin release were evaluated at varying glucose concentrations. At 3.3 mmol/l glucose level, the addition of 5.0 µmol/l glibenclamide or 5.0 µmol/l glibenclamide plus 200 µmol/l metformin caused a significant increase of insulin release, compared with glucose alone. At 16.7 mmol/l glucose concentration, a significant increase of insulin secretion, compared with glucose alone, was produced by the addition of either 5.0 µmol/l glibenclamide, 200 µmol/l metformin, or both 5.0 µmol/l glibenclamide and 200 µmol/l metformin. The effect of the combination of the two drugs was significantly higher than that with either drug used alone. Thus, glibenclamide was shown to have an insulinotropic effect on human islets at both low and high glucose concentrations, and metformin at high glucose concentrations. A possible synergistic effect of glibenclamide and metformin at high glucose concentrations is also suggested.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s005920050065

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

6

Digitale Medien

Insulin inhibits its own secretion from isolated, perifused human pancreatic islets (1995)

Marchetti, P. ; Scharp, D. W. ; McLear, M. ; [weitere]

Springer

Acta diabetologica 32 (1995), S. 75-77

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1432-5233

Schlagwort(e): Islets of Langerhans ; C-peptide

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract It is still a controversial question whether insulin suppresses its own secretion. We prepared pure human islets from three pancreases by collagenase digestion and density gradient purification. Aliquots of 200 islet equivalents (IE, 150-μm sized-islets) were sequentially perifused at 37°C with 3.3 mmol/l glucose (3.3G, 40 min), 16.7 mmol/l glucose (16.7G, 30 min) and again 3.3G (30 min) after 24 h, 37°C culture in CMRL 1066 medium with or without the addition of either 200 or 400 μU/ml human insulin in the incubation medium (6 replicates each). Insulin secretion was assessed by C-peptide (Cp) measurement in the perufusate. Without added insulin (C) and with 200 (Ins200) or 400 (Ins400) μU/ml added insulin, basal Cp release was 0.12±0.03, 0.14±0.02 and 0.14±0.04 ng/ml, respectively. At 16.7G, the first-phase secretion peak (expressed as Cp value) was significantly lower with Ins200 (0.47±0.13 ng/ml,P〈0.02) and Ins400 (0.68±0.15 ng/ml,P〈0.05) than C (0.83±0.15 ng/ml). The second-phase secretion peak was also significantly (P〈0.05) reduced with added insulin (Ins200: 0.47±0.08 ng/ml; Ins400: 0.45±0.07 ng/ml) than in its absence (C: 0.65±0.09 ng/ml). Accordingly, total Cp secretion was lower with Ins200 (10.6±2.3 ng/ml,P=0.03) and Ins400 (11.8±2.3 ng/ml) than with C (16.0±2.2 ng/ml). Thus, the addition for 24 h of either 200 or 400 μU/ml insulin in the culture medium caused a significant decrease of insulin (as assessed by Cp measurement) secretion from perifused human islets, suggesting that feedback suppression of insulin release is at least in part due to a direct action of insulin on the islets.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00569560

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext