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  • Articles: DFG German National Licenses  (53)
  • Chemistry  (50)
  • Peptide transport  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Characteristics of the protein carrier of the peptide-transport system in the scutellum of germinating barley embryos (1984)
    Springer
    Planta 162 (1984), S. 166-173 
    Details
    ISSN: 1432-2048
    Keywords: N-Ethylmalemide ; Germination (seed) ; Hordeum (embryo, peptide) ; Peptide transport ; Thiol group ; Transport carrier protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Through the use of the protein reagents N-ethylmaleimide, p-chloromercuribenzenesulphonic acid and phenylarsine oxide, it is shown that in the scutellum of the germinating barley embryo, the transport of peptides, but not the transport of amino acids or glucose is specifically thiol-dependent. Furthermore, these essential thiol groups are shown to exist as redox-sensitive, vicinal-dithiols that lie at the substrate-binding sites of the peptide-transport proteins. The binding of N-ethylmaleimide to these dithiols is shown to be very fast, matching the kinetics of inhibition of peptide transport by this reagent. A technique for the specific labelling of the dithiols with N-ethyl[2,3-14C]maleimide is described, which allows the carrier proteins to be visualized at the scutellar epithelium using radioautography and permits calculation of the approximate amount of peptide-transport protein present per scutellum. In related studies, the importance of arginyl and histidyl residues to both amino-acid and peptide transport is shown, although other residues, e.g. carboxyl ligands do not seem to be critically involved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410214
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characteristics of the active transport of peptides and amino acids by germinating barley embryos (1984)
    Springer
    Planta 162 (1984), S. 159-165 
    Details
    ISSN: 1432-2048
    Keywords: Amino acid transport ; Germination (seed) ; Hordeum (peptide) ; Peptide transport ; Scutellum ; Storage protein mobilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Use of two different assays involving either radioactively labelled substrates or a fluorescent-labelling procedure, gave good agreement for the rates of transport of peptides and amino acids into the scutellum of germinating grains of barley (Hordeum vulgare cv. Maris Otter, Winter). However, evidence was obtained for the enzymic decarboxylation of transpored substrate, which can cause underestimates of transport rates when using radioactively labelled substrates. The peptide Gly-Phe, was shown to be rapidly hydrolysed after uptake, and autoradiography of transported Gly-[U-14C]Phe indicated a rapid distribution of tracer, i.e. [U-14C] phenylalanine into the epithelium and sub-epithelial layers of the scutellum. The developmental patterns of transport activity indicate that peptide transport is more important nutritionally during the early stages of germination (1–3 d) whereas amino acids become relatively more important later (4–6 d). A range of amino acids is shown to be actively transported and several compete for uptake. At physiological concentrations, e.g. 2mM, transport of peptides and amino acids is inhibited about 80% by protonophore uncouplers, but at higher concentrations (10–100 mM) passive uptake predominates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410213
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  • 3
    Electronic Resource
    Electronic Resource
    Synthesis of the peptide-transport carrier of barley scutellum during the early stages of germination (1985)
    Springer
    Planta 164 (1985), S. 550-556 
    Details
    ISSN: 1432-2048
    Keywords: Germination (seed) ; Hordeum (peptide transport) ; Peptide transport ; Protein synthesis ; Scutellum ; Seed germination ; Thiol group
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Development of peptide-transport activity in the scutella of isolated barley (Hordeum vulgare l. cv. Maris Otter, Winter) embryos is shown to increase rapidly after about 15 h of imbibition, with the bulk of the transport activity being expressed by 24 h. This development is prevented by treatment of 15 h embryos with cycloheximide. Protein synthesis is found to increase in a closely related manner and also to be abolished by cycloheximide. Measurement of the incorporation of bound [35S]methionine by 15 to 21-h embryos indicates that de-novo protein synthesis during this period is greater in the scutellum than in the embryonic axis. Previous studies have shown that the peptide-transport system possesses essential dithiol groups, probably located at the substrate-binding site (Walker-Smith and Payne 1983 b, 1984b). Treatment of 15-h embryos with the non-penetrant thiol reagent p-chloromercuribenzene sulphonic acid did not affect development of peptide-transport activity during the following 6 h, whereas with 3-d embryos identical treatment inhibited uptake almost completely during a subsequent 6-h period. Radioautography revealed that amongst the proteins synthesised during this early phase of germination and labelled in vitro with [35S]methionine some are found within the epithelial plasmalemmae of the scutellum, which is the location of the peptide-transport carrier identified previously by externally labelling with a radioactive thiol reagent. The results provide evidence that protein(s) of the peptide-transport system are synthesised and inserted into the scutellum during early germination, allowing the system to play a major role in the nitrogen nutrition of the embryo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395974
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Further aspects of the thermal degradation of epoxide resins (1967)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 11 (1967), S. 1009-1026 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: This paper describes an investigation into the degradation of a purified epoxide based on the diglycidyl ether of bisphenol A hardened with p,p′-diaminodiphenylmethane. The method used was that of hot-wire pyrolysis followed by gas chromatography. Special attention was given to the problem of solid residues formed on the pyrolyzer tube, and evidence was found that these probably contain oligomers. Resonance-stabilized free radicals also appear to be formed, and evidence is found to support the idea of dehydration during degradation, originally put forward by Lee. An attempt, based on first principles, is made to explain the degradation of epoxides, using evidence from previous work as well as that described in this paper.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1967.070110701
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  • 5
    Electronic Resource
    Electronic Resource
    Combined pyrolysis and radiochemical gas chromatography for studying the thermal degradation of epoxy resins and polyimides. I. The degradation of epoxy resins in nitrogen between 400°C and 700°C (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 14 (1970), S. 205-223 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The thermal degradation of a bisphenol A-based epoxy resin (EP 274) cured with 4,4′-diaminodiphenyl methane (DDM) and with phthalic anhydride (PA) was studied using a radiochemical pyrolysis gas chromatography technique. Conclusive evidence for some of the degradation mechanisms of these resins was obtained by pyrolyzing samples containing various 14C-labelled groups and analyzing the products using this method.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1970.070140120
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  • 6
    Electronic Resource
    Electronic Resource
    Über die Einwirkung von Titansäureanhydrid auf Natriumkarbonat (1903)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 37 (1903), S. 332-336 
    Details
    ISSN: 0863-1778
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19030370124
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  • 7
    Electronic Resource
    Electronic Resource
    Metallographische Mitteilungen aus dem lnstitut für anorganischs Chemie der Universität Göttingen. Über die Legierungen des Kaliums mit Aluminium, Magnesium, Zink, Cadmium, Wismut, Zinn und Blei. Einleitung (1907)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 56 (1907), S. 109-142 
    Details
    ISSN: 0863-1778
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19070560108
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  • 8
    Electronic Resource
    Electronic Resource
    Radical initiated homo- and copolymerization of N,N,N′N′-tetra(2-hydroxypropyl)ethylene diamine methacrylate (1990)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 28 (1990), S. 949-953 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1990.080280421
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  • 9
    Electronic Resource
    Electronic Resource
    Preservation of potential fermentables in sweet sorghum by ensiling (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 860-867 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pressed and wilted samples of sweet sorghum [Sorghum bicolor (L.) Moench var. Rio] were ensiled for periods up to 155 days. A kinetic study of the biochemical changes which occurred during ensiling showed that in wilted sorghum ensilage invert sugars and mannitol levels collectively were maintained at 65% of the original ferment able sugar content of the sorghum. The acidic environment produced by ensiling also served as a pretreatment that resulted in enhanced yields of reducing sugar when the sorghum was contacted with cellulolytic enzymes. The quantity of sugar obtained from enzymatic hydrolysis more than compensated for carbohydrate used by organisms during the ensiling process. Both Saccharomyces uvarum and Clostridium acetobutylicum were able to ferment a medium constituted from pressed sorghum juice and the solution resulting from enzymatic hydrolysis of sweet sorghum ensilage.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260300708
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  • 10
    Electronic Resource
    Electronic Resource
    Gas and solids behavior in a baffled and unbaffled slurry bubble column (1987)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 33 (1987), S. 1959-1970 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: New data and analyses are presented for describing gas and solids behavior in a slurry bubble column. Axial solids concentration and bubble size distributions were measured in a 0.108 m ID slurry bubble column apparatus operated at steady state conditions. The column was operated with and without internal baffles. The slurry and gas superficial velocities ranged from 0.0077 to 0.009 m/s and from 0.031 to 0.24 m/s, respectively. The solid phase consisted of glass spheres in a narrow size range with a mean diameter of 96.5 μm; the liquid phase consisted of tap water.With a one-dimensional sedimentation-dispersion model, the data have been used to predict average solids loadings and axial distributions of the solids for specified operating conditions. Local bubble size and concentration measurements indicate that the slurry bubble column was operated in the churn-turbulent flow regime throughout the entire range of operating conditions used in this study. The impact of internal baffles and solids concentration on the gas and solids behavior is discussed.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690331204
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