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  • Articles: DFG German National Licenses  (2)
  • Chemosensitivity  (1)
  • Dopplersonographie  (1)
  • 1
    ISSN: 1432-1335
    Keywords: Gynecological tumors ; Cellular heterogeneity in culture ; Chemosensitivity ; Proliferation assay ; Reproducibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Procedures for the in vitro determination of the drug-induced inhibition of mammary and ovarian carcinoma cell growth were established. In monolayer cultures derived from advanced tumors, separation of epithelial carcinoma cells from concomitant cells of fibroblast-like or mesothelial appearance was achieved by differential trypsinization. The carcinoma cell character of the stock cultures was verified by chromosome analyses showing a high degree of aneuploidy for the epitheloid cell lines and euploidy for cells of apparently mesenchymal origin. When cultured carcinoma cells were injected in nu/nu mice, the tissue and cell cultures obtained from the heterotransplantation tumors closely resembled the original tumors and cell cultures in morphology, karyotype, and expression of tumor markers. The action of carcinostatic drugs in the logarithmic phase of the carcinoma cell proliferation was tested by kinetics experiments in multiple experimental cultures. In cell proliferation assays based on cell counts the 50% inhibition dose (ID50) of the drug effects was determined from the dose-response curves. Comparison of the ID50s revealed highly differential effectiveness of the drugs examined. The inhibitory effects were reproducible, rendering the procedures used suitable for testing the chemosensitivity of newly explanted gynecological carcinoma cells by proliferation assays.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2102
    Keywords: Schlüsselwörter High-flow-Priapismus ; Perineales Trauma ; Superselektive Embolisation ; Dopplersonographie ; Angiographie ; Key words High-flow priapism ; Perineal trauma ; Superselective embolization ; Dopplerultrasound ; Angiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A 10-year old boy developed an arteriocavernosal fistula with a permanent penile erection (high-flow priapism) after a blunt perineal trauma. The diagnosis was established by Doppler ultrasound, blood gas analysis and angiography. A conservative treatment attempt with penile cooling and puncture of the cavernosal corpera with blood aspiration and etilefrine instillation failed to resolve the priapism. An embolization of both bulbocavernosal arteries was followed by an immediate detumescence. Normal functional erection was preserved. No recurrence was observed during a follow-up of 6 months.
    Notes: Zusammenfassung Bei einem 10jährigen Jungen kam es nach einem stumpfen perinealen Trauma zu einer Dauererektion durch eine arteriokavernöse Fistel (High-flow-Priapismus), die durch Doppler-Sonographie, Blutgasanalyse und Angiographie nachgewiesen wurde. Nach Versagen einer Behandlung mit Kühlung, Dekompression der Corpora cavernosa durch Punktion und Etilefrin-Instillationen wurde eine endovasale Verschlußbehandlung der die Fistel speisenden Aa. bulbourethrales mit Mikrocoils vorgenommen. Die Behandlung führte zu einer sofortigen Detumeszenz bei erhaltener Erektionsfähigkeit. Während einer Nachbeobachtungszeit von 6 Monaten trat kein Rezidiv des Priapismus auf.
    Type of Medium: Electronic Resource
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