ISSN:
1617-4623
Keywords:
Key wordsStreptomyces
;
Ordered cosmid map
;
afsA gene
;
Chromosomal deletions
;
Circularization of chromosome
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have recently constructed a physical map of the Streptomyces griseus 2247 genome using the restriction enzymes AseI and DraI, which revealed that this strain carries a 7.8 Mb linear chromosome. Based on this map, precise macrorestriction fragment and cosmid maps were constructed for both ends of the chromosome, which localized the afsA gene 150 Kb from the left end. Two afsA − mutants were found to have suffered chromosomal deletions that removed the afsA locus. The sizes of the deletions were 20 and 130 Kb at the right end and 180 and 350 kb at the left end, respectively. Hybridization experiments using cosmids carrying a deletion endpoint indicated that the ends of the chromosome in the mutants were fused to form a circular chromosome.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380050346
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