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  • Articles: DFG German National Licenses  (3)
  • Cytoplasmic male sterility  (3)
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  • Articles: DFG German National Licenses  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    The use of mitochondrial DNA polymorphism in the classification of individual onion plants by cytoplasmic genotypes (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 345-348 
    Details
    ISSN: 1432-2242
    Keywords: Onion ; Allium cepa ; Cytoplasmic male sterility ; Mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Individual plants of a Japanese onion variety ‘Sapporo-ki’, which is characterized by the occasional occurrence of male-sterile plants, have been investigated for mitochondrial (mt) DNA polymorphism. Male-fertile and the Jones' cytoplasmic male-sterile (CMS) onions were also included for comparison. Southern blot hybridization with rrn26, cox-I, cox-II, cob, atpA and atp9 genes as probes revealed the two classes of mtDNA variation within a population of ‘Sapporo-ki’: Out of the 41 plants examined 19 contained mtDNA typical of malefertile plants, and 22 individuals contained mtDNA typical of the Jones' CMS genotype. Our results thus indcate that the use of the mitochondrial gene probes may greatly facilitate the classification of individual plants by cytoplasmic genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222100
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Alterations in organization and transcription of the mitochondrial genome of cytoplasmic male sterile sugar beet (Beta vulgaris L.) (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 283-290 
    Details
    ISSN: 1617-4623
    Keywords: Key words Sugar beet ; Cytoplasmic male sterility ; Mitochondrial genome ; Genome rearrangement ; Rf gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have constructed a physical map of the mitochondrial DNA of a cytoplasmic male sterile (CMS) sugar beet line, TK81-MS, and compared it with that published for normal fertile sugar beet (cv. TK81-O) to clarify the differences between the CMS and normal mitochondrial genomes. The TK81-MS genome is present as a single circular molecule of 481.8 kb, or as two molecules of 184.9 and 296.9 kb. The CMS genome was found to be highly rearranged relative to the normal mitochondrial genome, with at least fifteen rearrangement and/or inversion events being required to align the two DNAs. Analysis of transcription patterns of known mitochondrial genes and rearranged regions revealed six genes, coxI, coxII, atpA, atp6, rps3, and orf324, whose expression is altered in the CMS line relative to the normal line. Of these six, only the coxI transcript pattern differs between male-sterile and fertility-restored genotypes, making it likely that the coxI locus is involved in mediating CMS in sugar beet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051085
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Organelle genome diversity in sugar beet with normal and different sources of male sterile cytoplasms (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 166-171 
    Details
    ISSN: 1432-2242
    Keywords: Sugar beet ; Cytoplasmic male sterility ; Mitochondrial and chloroplast DNAs ; Restriction endonuclease fragment analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) and chloroplast (ct) DNAs from sugar beet lines carrying normal and introduced sources of male sterile cytoplasms have been characterized and compared on the basis of restriction enzyme analysis. Normal cytoplasm was shown to contain mt and ctDNAs which differed from those of the male sterile cytoplasms examined in the present investigation. On the other hand, four groups of male sterile cytoplasms could be differentiated by their own characteristic mtDNA digest patterns, while two were separated by ctDNA comparisons. In addition, a greater degree of variability of the mitochondrial genome is suggested. Our results also imply strict maternal inheritance of mt and ctDNAs. Thus, the organelle DNA assay provides a positive and alternative means of identifying various male sterile cytoplasms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252051
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    Paper (German National Licenses)
    Fulltext
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