ISSN:
1573-5028
Keywords:
cis-control element
;
gel retardation
;
DNase I footprinting
;
rDNA promoter
;
mutation analysis
;
Vicia faba
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site of the pre-rRNA transcript. This location does not correspond with previously reported results on maize rRNA genes. However, both of the binding sites share a bi-partite consensus sequence, TAT-G(N)xCAGG. Methylation interference experiments show that two G residues in TATG and the complementary strand of CAGG are important for specific DNA-protein interaction. Furthermore, competition analyses using point-mutated synthetic DNAs show that two G residues in CAGG are essential for this interaction. Similar sequences are found in promoter regions of other plant and animal rRNA genes. We suggest that these sequences may be a cis-control element commonly involved in rRNA transcription.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00027164
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