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  • 1
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Journal of metastable and nanocrystalline materials Vol. 19 (Jan. 2004), p. 1-24 
    ISSN: 1422-6375
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 607 (Nov. 2008), p. 70-72 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Positron annihilation lifetime spectroscopy (PALS) measurements were performed on polymer electrolyte membranes (PEMs) synthesized by radiation-induced graft polymerization of styrene into crosslinked-polytetrafluoroethylene (cPTFE) films and subsequent sulfonation. The base cPTFE and polystyrene grafted films were also measured as references. The cPTFE based PEM was found to have free-volume holes with different radius of 0.28-0.30 nm and 0.44-0.45 nm. The larger holes may be located in PTFE amorphous regions, while the smaller ones are considered to exist in both PTFE crystallites and poly(styrene sulfonic acid) grafts containing no water
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 426-432 (Aug. 2003), p. 3073-3078 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 475-479 (Jan. 2005), p. 1445-1448 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A V-4Cr-4Ti alloy and pure V were oxidized in flowing argon for 0.5-8h andsubsequently annealed in vacuum for 16h at 973K. The oxygen of 1000-9000ppm was introduced into the V-alloys with little nitrogen, where oxygen was concentrated in region near the surface. By the annealing, oxygen was homogeneously diffused into depth of 150µm. The diffusion depth of oxygen in V-4Cr-4Ti is much smaller than that of pure V, because of Ti-O precipitates formed as oxygen trap. The study indicated that it is feasible to control oxygen level and distribute in the surface region of the vanadium alloys
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 426-432 (Aug. 2003), p. 667-672 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 449-452 (Mar. 2004), p. 1265-1268 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Microstructures of Ti-29Nb-13Ta-4.6Zr (TNTZ) aged at temperatures between 573 and723 K after solution treatment at 1063 K have super fine omega phase, or?? both super fine alphaand omega phases, respectively in beta phase with an average grain diameter of 20 µm. Plain fatiguestrength of TNTZ aged after solution treatment is much greater than that of as-solutionized TNTZ inboth low cycle fatigue and high cycle fatigue life regions. This is due to the improvement of thebalance of strength and ductility by the precipitation of alpha phase. Fretting fatigue strength ofTNTZ conducted with various heat treatments decreases dramatically as compared with their plainfatigue strength in both low cycle fatigue and high cycle fatigue life regions. In this case, thedecreasing ratio of fretting fatigue life increases with increasing the small crack propagation areawhere both the tangential force and frictional force at the contact plane of pad exist. In frettingfatigue in air, the ratio of fretting damage (Pf/Ff), where Pf and Ff stand for plain fatigue limit andfretting fatigue limit, respectively, increases with increasing elastic modulus. In fretting fatigue inRinger’s solution, the passive film on specimen surface is broken by fretting action in TNTZ, whichhave excellent corrosion resistance, and, as a result, corrosion pits that lead to decreasing frettingfatigue strength especially in high cycle fatigue life region, are formed on its surface
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1327
    Keywords: Key words Fluorine-19 nuclear magnetic resonance ; Heme electronic structure ; Myoglobin ; Paramagnetic shift ; Reconstituted protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract  A novel C 2-symmetric ring-fluorinated hemin, 13,17-bis(2-carboxyethyl)-2,8,12,18-tetramethyl-3,7-difluoroporphyrinatoiron(III), has been synthesized and was incorporated into sperm whale apomyoglobin to investigate protein-induced rhombic perturbations on the electronic structure of the active site of myoglobin (Mb) using 19F NMR spectroscopy. NMR signals for 19F atoms introduced as substituents on the present heme in ferrous low-spin and high-spin and ferric low-spin complexes have been observed and their shifts sharply reflect not only the electronic nature of the heme iron, but also in-plane asymmetry of the heme electronic structure. The two-fold symmetric electronic structure of the ring-fluorinated hemin is clearly manifested in the 19F and 1H NMR spectra of its dicyano complex. The chemical equivalence of the two fluorine atoms of the heme is removed in the active site of myoglobin and the splitting of the two 19F NMR signals provides a quantitative probe for characterizing the rhombic perturbation of the heme electronic structure induced by the heme-protein interaction. The in-plane asymmetry of heme electronic structures in carbonmonoxy and deoxy Mbs have been analyzed for the first time on the basis of the shift difference between the two 19F NMR signals of the heme and is interpreted in terms of iron-ligand binding and/or the orbital ground state of the heme. A potential utility of 19F NMR, combined with the use of a symmetric fluorinated hemin, in characterizing the heme electronic structure of myoglobin in a variety of iron oxidation, spin, and ligation states, is presented.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5028
    Keywords: cis-control element ; gel retardation ; DNase I footprinting ; rDNA promoter ; mutation analysis ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site of the pre-rRNA transcript. This location does not correspond with previously reported results on maize rRNA genes. However, both of the binding sites share a bi-partite consensus sequence, TAT-G(N)xCAGG. Methylation interference experiments show that two G residues in TATG and the complementary strand of CAGG are important for specific DNA-protein interaction. Furthermore, competition analyses using point-mutated synthetic DNAs show that two G residues in CAGG are essential for this interaction. Similar sequences are found in promoter regions of other plant and animal rRNA genes. We suggest that these sequences may be a cis-control element commonly involved in rRNA transcription.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-5028
    Keywords: AACA motif ; ACGT motif ; cis-regulatory elements ; endosperm expression ; GCN4 motif ; glutelin gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rice storage protein glutelin genes are coordinately regulated during seed development. A previous 5′ deletion analysis using transgenic tobacco revealed that the minimum 5′ region necessary for endosperm specificity was within −245 bp of the transcription start site, and included the AACA and GCN4 motifs that are highly conserved in the 5′-flanking regions of all glutelin genes. In this paper, the sequence elements essential for endosperm-specific expression are characterized in stable transgenic tobacco plants by both loss-of-function and gain-of-function experiments using this minimum promoter. Base substitution analysis shows that the proximal AACA motif between −73 and −61, and the GCN4 motif between −165 and −158 act as critical elements. An ACGT motif between −81 and −75, and Skn-I-like elements between −173 and −169 also play important roles in controlling the seed-specific expression. When the distal region between −245 and −145 containing the AACA and the GCN4 motifs or the proximal region between −113 and −46 containing the ACGT and AACA motifs is fused to a truncated promoter (−90 to +9) of the CaMV 35S gene fused to the β-glucuronidase (GUS) reporter gene, high levels of seed-specific expression are observed in these fusions, thereby indicating that either pair of motifs is sufficient to confer seed expression in these fusions. However, when substituted for by the CaMV 35S core promoter (−46 to +1), seed expression is abolished, suggesting that the sequence between −90 and −46 of the CaMV 35S promoter containing G-box-like motif (as-1 element) is required for such specific expression in addition to AACA and GCN4 motifs. Therefore, we conclude that at least three cis-regulatory elements, the AACA motif, GCN4 motif and ACGT motif, are necessary to mediate endosperm expression of the GluB-1 glutelin gene.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1618-0860
    Keywords: Keywords: Condensation pattern, Fluorescence in situ hybridization (FISH), Genome size, Lotus japonicus, Quantitative chromosome map, rDNAs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Lotus Japonicus , Miyakojima MG-20 and Gifu B-129. The genome sizes of Miyakojima and Gifu were determined as 472.1 and 442.8 Mbp, respectively. Both the accessions were diploid (2n=12) and six chromosomes were identified and characterized based on the condensation patterns and the locations of rDNA loci. The obvious polymorphism observed in the genome size and the chromosome morphology between the two accessions, revealed specific accumulation of heterochromatin in Miyakojima or elimination in Gifu. The chromosomes L. japonicus were numbered according to their length. A quantitative chromosome map was also developed by the imaging methods using the digital data of the condensation pattern. 45S rDNA loci were localized on chromosomes A and F, and 5S rDNA locus was localized on chromosome A by fluorescence in situ hybridization (FISH). Identification of the chromosome and genome sizes and development of the quantitative chromosome map represent significant contribution to the L. japonicus genome project as the basic information.
    Type of Medium: Electronic Resource
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