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  • Articles: DFG German National Licenses  (3)
  • Escherichia coli  (2)
  • Life and Medical Sciences  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Catabolite regulation of two Escherichia coli operons encoding nitrite reductases: role of the Cra protein (1997)
    Springer
    Archives of microbiology 168 (1997), S. 240-244 
    Details
    ISSN: 1432-072X
    Keywords: Key words Catabolite regulation ; Cra protein ; Nitrite ; reductase regulation ; Escherichia coli ; Catabolite ; repression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Escherichia coli nir and nrf operons, which encode alternative nitrite reductases expressed during anaerobic growth, are subject to catabolite regulation. Transcription from the nir promoter is maximal when bacteria are grown in rich media such as Lennox broth supplemented with glucose. Conversely, expression of the nrf operon is suppressed by rich media, but stimulated during growth in minimal medium with glycerol and fumarate. The role of the catabolite repressor-activator (Cra) protein in catabolite regulation of the nir and nrf promoters was investigated. Transcription from the nir promoter was repressed by Cra when cells were grown in minimal medium with glycerol and fumarate. Crude protein extracts from a strain overproducing Cra encoded on a multicopy plasmid retarded a nir promoter fragment in a mobility shift assay, confirming that the observed Cra-dependent repression was due to the direct interaction of Cra with the regulatory region of the nir operon. Furthermore, the inclusion of fructose 1-phosphate, an effector of Cra DNA-binding activity, in the assay decreased the ability of Cra to retard the nir promoter fragment. In contrast, transcription from the nrf promoter was not regulated by Cra under any of the growth conditions tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050494
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of mutations in genes for proteins involved in disulphide bond formation in the periplasm on the activities of anaerobically induced electron transfer chains in Escherichia coli K12 (1996)
    Springer
    Molecular genetics and genomics 253 (1996), S. 95-102 
    Details
    ISSN: 1617-4623
    Keywords: Key words Disulphide bond formation ; Cytochrome c assembly ; Nitrite reduction ; Nitrate reduction ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The assembly of anaerobically induced electron transfer chains in Escherichia coli strains defective in periplasmic disulphide bond formation was investigated. Strains deficient in DsbA, DsbB or DipZ (DsbD) were unable to catalyse formate-dependent nitrite reduction (Nrf activity) or synthesize any of the known c-type cytochromes. The Nrf+ activity and cytochrome c content of mutants defective in DsbC, DsbE or DsbF were similar to those of the parental, wild-type strain. Neither DsbC expressed from a multicopy plasmid nor a second mutation in dipZ (dsbD) was able to compensate for a dsbA mutation by restoring nitrite reductase activity and cytochrome c synthesis. In contrast, only the dsbB and dipZ (dsbD) strains were defective in periplasmic nitrate reductase activity, suggesting that DsbB might fulfil an additional role in anaerobic electron transport. Mutants defective in dipZ (dsbD) were only slightly more sensitive to Cu++ ions at concentrations above 5 mM than the parental strain, but strains defective in DsbA, DsbB, DsbC, DsbE or DsbF were unaffected. These results are consistent with our earlier proposals that DsbA, DsbB and DipZ (DsbD) are part of the same pathway for ensuring that haem groups are attached to the correct pairs of cysteine residues of apocytochromes c in the E. coli periplasm. However, neither DsbE nor DsbF are essential for the reduction of DipZ (DsbD).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00013815
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Relationship of deep structures of the hand and wrist to topographical landmarks (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Clinical Anatomy 6 (1993), S. 300-307 
    Details
    ISSN: 0897-3806
    Keywords: carpal canal ; carpal tunnel surgery ; carpal tunnel syndrome ; flexor retinaculum ; transverse carpal ligament ; Life and Medical Sciences ; Miscellaneous Medical
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The relationship of topographical landmarks to the deep structures of the wrist and hand has not been clearly defined and would be of benefit to surgeons performing endoscopic carpal tunnel surgery. To this end, 10 fresh cadaveric wrist specimens had radiopaque markers placed adjacent to key anatomic structures within the wrist and hand. A new technique for localizing the hook of the hamate was established. We found that the thick segment of the flexor retinaculum, often defined as the transverse carpal ligament, was 2.2 cm in length. Its distal border was 1 cm distal to the distal aspect of the hook of the hamate. The proximal ligamentous portion had a mean length of 1.2 cm, whereas the distal aponeurotic portion (distal to the hook of the hamate) had a mean length of 1.0 cm. The position of the superficial palmar arch was 2.7 cm distal to the hook of the hamate or at a line bisecting three-fourths of the distance from the distal flexor wrist crease to the proximal palmar crease. The relationship of the superficial palmar arch to topographical landmarks was more consistent than it was when the standard reference of Kaplan's cardinal line was used. © 1993 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ca.980060507
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    Paper (German National Licenses)
    Fulltext
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