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  • 1
    ISSN: 1432-072X
    Keywords: Key words Nitrite reductase mutants ; Formate-dependent nitrite reduction ; Anaerobic electron transfer ; Menaquinones ; hemN ; Cytochrome c biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three Escherichia coli mutants defective in formate-dependent nitrite reduction (Nrf activity) were characterised. Two of the mutants, JCB354 and JCB356, synthesized all five c-type cytochromes previously characterised in anaerobic cultures of E. coli. The third mutant, JCB355, was defective for both cytochrome b and cytochrome c synthesis, but only during anaerobic growth. The insertion sites of the transposon in strains JCB354 and JCB356 mapped to the menFDBCE operon; the hemN gene was disrupted in strain JCB355. The mutation in strain JCB354 was complemented by a plasmid encoding only menD; strain JCB356 was complemented by a plasmid encoding only menBCE. A mutant defective in the methyltransferase activity involved in both ubiquinone synthesis and conversion of demethylmenaquinone to menaquinone expressed the same Nrf activity as the parental strain. The effects of men, ubiA and ubiE mutations on other cytochrome-c-dependent electron transfer pathways were also determined. The combined data establish that menaquinones are essential for cytochrome-c-dependent trimethylamine-N-oxide reductase (Tor) and Nrf activity, but that either menaquinone or ubiquinone, but not demethylmenaquinone, can transfer electrons to a third cytochrome-c-dependent electron transfer chain, the periplasmic nitrate reductase.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 168 (1997), S. 240-244 
    ISSN: 1432-072X
    Keywords: Key words Catabolite regulation ; Cra protein ; Nitrite ; reductase regulation ; Escherichia coli ; Catabolite ; repression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Escherichia coli nir and nrf operons, which encode alternative nitrite reductases expressed during anaerobic growth, are subject to catabolite regulation. Transcription from the nir promoter is maximal when bacteria are grown in rich media such as Lennox broth supplemented with glucose. Conversely, expression of the nrf operon is suppressed by rich media, but stimulated during growth in minimal medium with glycerol and fumarate. The role of the catabolite repressor-activator (Cra) protein in catabolite regulation of the nir and nrf promoters was investigated. Transcription from the nir promoter was repressed by Cra when cells were grown in minimal medium with glycerol and fumarate. Crude protein extracts from a strain overproducing Cra encoded on a multicopy plasmid retarded a nir promoter fragment in a mobility shift assay, confirming that the observed Cra-dependent repression was due to the direct interaction of Cra with the regulatory region of the nir operon. Furthermore, the inclusion of fructose 1-phosphate, an effector of Cra DNA-binding activity, in the assay decreased the ability of Cra to retard the nir promoter fragment. In contrast, transcription from the nrf promoter was not regulated by Cra under any of the growth conditions tested.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0375-9474
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Expression from both the Escherichia coli nir and nrf promoters is dependent on anaerobic induction by FNR but is further regulated by NarL and NarP in response to the presence of nitrite and nitrate in the growth medium. The nir promoter is activated by NarL in response to nitrate and nitrite and activated by NarP in response to nitrate but not nitrite. The effects of point mutations suggest that NarL and NarP both bind to the same target, which is a pair of heptamer sequences organized as an inverted repeat, centred 691/2 bp upstream of the transcript startpoint. The nrf promoter can be activated by either NarP or NarL in response to nitrite but is repressed by NarL in response to nitrate. Mutational analysis of the nrf promoter has been exploited to corroborate the location of the -10 hexamer and the FNR-binding site, and to find the sites essential for nitrite-dependent activation and nitrate-dependent repression. Optimal activation by NarP or NarL in response to nitrite requires an inverted pair of heptamer sequences, similar to that found at the nir promoter, but centred 741/2 bp upstream from the transcript start. NarL-dependent repression by nitrate is due to two heptamer sequences that flank the FNR-binding sequence. We conclude that NarL and NarP bind to the same heptamer sequences, but that the affinities for the two factors vary from site to site.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Transcription initiation at the Escherichia coli nirB promoter is induced by anaerobic growth and further increased by the presence of nitrite or nitrate in the growth medium. Expression from this promoter is totally dependent on the transcription factor, FNR, which binds between positions −52 and −30 upstream of the transcription startsite. The 20 base pairs from position −79 to −60 contain an inverted repeat of two 10-base sequence elements that are related to sequences at the NarL-binding site at the E. coli narG promoter. Comparison of these, and sequence elements at other promoters regulated by NarL, suggests a consensus NarL-binding sequence. Mutations in the putative NarL-binding site at the nirB promoter decrease FNR-dependent anaerobic induction, suggesting that NarL acts as a helper to FNR during transcription activation. These mutations also suppress induction by nitrite: single mutations at symmetry-related positions have similar effects, whilst double mutations have more severe effects, probably because two NarL subunits bind to the inverted repeat. Disruption of narL decreases nitrite induction of the nirB promoter whilst not suppressing induction by nitrate, suggesting that there may be a second nitrate-responsive factor. Nitrate induction was, however, suppressed by double mutations at symmetry-related positions in the NarL-binding site, suggesting that this putative second factor may bind to sequences similar to those recognized by NarL.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 30 (1975), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Variations in soil-water and plant-water status were examined in swards of perennial ryegrass over a period of 2 years. Unirrigatcd swards were compared with swards partially irrigated after cutting and with swards fully irrigated after cutting and then again whenever a 25 mm potential soil water deficit (SWD) arose. Two levels of N were applied to the swards (Nj= 250 kg N/ha, NT= 500 kg N/ha in each year). Frequent measurement of the water in the soil profiles beneath these swards with a neutron soil-moisture probe, enabled comparisons to be made of the actual SWD with the potential SWD on which the irrigation regimes were based. In the unirrigated swards the actual rate of water use by the swards fell below the potential rate of use at a potential SWD of approximately 50 mm. On the other hand, frequent irrigation at a potential SWD of 25 mm often failed to maintain the actual deficit within 25 mm of field capacity. Under both the unirrigated and the partially irrigated swards, greater use was made of the soil water below 30 cm depth in the N2 treatment than in the N1.The pressure-chamber technique proved particu larly suitable for measuring the variation in leaf-water potential (LWP) of grasses in the field. LWP was highest (least negative) at dawn and, provided that no dew was present on the leaves, was related to the actual SWD. There was no direct relationship between the daily minimum value of LWP and SWD, the former being determined largely by evaporative demand. However, LWP in frequently irrigated swards rarely fell below –15 atm, whereas much lower values were recorded in swards on drier soils. It is suggested that the effect of irrigation on plant-water stress may be most readily assessed from the LWP at dawn.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 47 (1992), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A long-term field grazing experiment was begun in 1982 to examine the impact of efficient field drainage on herbage and animal production from swards on an impermeable clay loam in the south-west of England. Drained and undrained lysimeter plots (each of 1 ha) were established on the existing permanent sward and received annual applications of fertilizer N of 200 or 400 kg ha−1. Similar plots were initially ploughed and reseeded with Lolium perenne (cv. Melle), and received fertilizer N at an annual rate of 400 kg ha−1. All plots were continuously stocked by beef cattle and stock numbers were adjusted to maintain a constant sward height and to avoid poaching damage. Results for the first 5 years show that the benefits from drainage were modest and, for beef production, unlikely to pay for its costs over the shorter term. The main benefit was in spring when herbage dry matter yield was 11% greater on the drained plots, but with no significant interaction with fertilizer N level or sward type. This benefit was reduced to 3% on an annual basis, due to the effect of the larger soil water deficits sustained by the drained swards in mid-season. Drainage increased the annual liveweight gain per ha by the grazing cattle by 11%. Possible mechanisms accounting for these effects are discussed in relation to the influences of seasonal patterns of weather.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 28 (1973), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The response of established perennial ryegrass swards to irrigation was compared over 2 years. The swards received 250 (N1) or 500 kg N/ha (N2) and were cut 4 times each year. Water was applied in 5 irrigation treatments according to the potential soil water deficit: the soil was partially, or fully returned to field capacity after cutting; returned to field capacity after cutting and then whenever a 25 mm deficit arose; irrigated with 25 mm water whenever a 25 mm deficit arose independently of cutting; or irrigated at minimum intervals of 14 days, provided that the deficit was then at least 50 mm.There was little effect of irrigation on yield from the first cut in late May. Frequent irrigation gave the greatest increase in total annual yield (3.1 t/ha), hut partial irrigation after cutting was most efficient in use of water and equipment, provided that drought conditions were avoided.High concentrations of N in the herbage were associated with low DM yields, hut more N (percentage N DM yield) was recovered from the irrigated than from the unirrigated swards. More N was recovered than was applied in the N1 treatment; less was recovered than was applied in N2. In the N1 treatment the nitrate concentration was 〈500 ppm hut it was 1000–4000 ppm in N2. There was no consistent effect of irrigation on nitrate or K concentration.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1617-4623
    Keywords: Key words Disulphide bond formation ; Cytochrome c assembly ; Nitrite reduction ; Nitrate reduction ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The assembly of anaerobically induced electron transfer chains in Escherichia coli strains defective in periplasmic disulphide bond formation was investigated. Strains deficient in DsbA, DsbB or DipZ (DsbD) were unable to catalyse formate-dependent nitrite reduction (Nrf activity) or synthesize any of the known c-type cytochromes. The Nrf+ activity and cytochrome c content of mutants defective in DsbC, DsbE or DsbF were similar to those of the parental, wild-type strain. Neither DsbC expressed from a multicopy plasmid nor a second mutation in dipZ (dsbD) was able to compensate for a dsbA mutation by restoring nitrite reductase activity and cytochrome c synthesis. In contrast, only the dsbB and dipZ (dsbD) strains were defective in periplasmic nitrate reductase activity, suggesting that DsbB might fulfil an additional role in anaerobic electron transport. Mutants defective in dipZ (dsbD) were only slightly more sensitive to Cu++ ions at concentrations above 5 mM than the parental strain, but strains defective in DsbA, DsbB, DsbC, DsbE or DsbF were unaffected. These results are consistent with our earlier proposals that DsbA, DsbB and DipZ (DsbD) are part of the same pathway for ensuring that haem groups are attached to the correct pairs of cysteine residues of apocytochromes c in the E. coli periplasm. However, neither DsbE nor DsbF are essential for the reduction of DipZ (DsbD).
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric radiology 10 (1981), S. 201-205 
    ISSN: 1432-1998
    Keywords: Pyloric stenosis ; Pylorospasm ; Gastric outlet obstruction ; Infant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Short segment narrowing of the pyloric canal is a common finding in infants with chronic vomiting, and most often is due to pylorospasm. In such cases, it is transient, and offers no real problem in diagnosis. On the other hand, when it persists, a question arises as to whether it is due to fixed stenosis. Differentiation of the two conditions is difficult, but important, for while spasm can be treated medically, true stenosis requires surgical intervention. Just how to accomplish this differentiation is the subject of this report, and for the most part, centers around the infant's response to a therapeutic trial of antispasmodics. Patients with pylorospasm respond favorably, while those with fixed stenosis do not. Generally, these latter patients require polyromyotomy, but in the occasional infant, so little muscle hypertrophy is present that we have found pyloroplasty to be the preferred procedure.
    Type of Medium: Electronic Resource
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