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  • Articles: DFG German National Licenses  (3)
  • Gene transcription  (2)
  • Glycine max  (1)
  • 1
    ISSN: 1432-1327
    Keywords: Key words Transcription factor IIB ; Metalloprotein ; Zinc finger ; Gene transcription ; X-ray absorption spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract X-ray absorption spectroscopy has been used to compare the metal coordination of the N-terminal zinc binding domain of eukaryal human transcription factor (TF) IIB to the previously reported structure of archaeal Pyrococcus furiosus (Pf) TFB. Full length and N-terminal fragments for both PfTFBand human TFIIB were cloned, expressed, and purified. The [C10H] variant of PfTFB was constructed to resemble the metal binding motif of higher eukaryal TFIIB proteins by mutating the second cysteine ligand to a histidine. All five proteins bind zinc in a 1 :1 ratio. Zn X-ray absorption spectroscopy of human TFIIB and [C10H]PfTFB mutant are consistent with ZnS3(N,O) ligation, and further suggest that the N/O ligand is an imidazole.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1327
    Keywords: Key words TFIIB ; Metalloprotein ; Zinc finger ; Gene transcription ; X-ray absorption spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract  The gene for an archaeal homolog of the eukaryotic transcription factor TFIIB has been cloned from the marine hyperthermophilic archaeon Pyrococcus furiosus and overexpressed in Escherichia coli. This TFB gene displays a sequence that is identical to a gene sequence in P. woesei. A gene for the 49-residue N-terminal domain of TFB that contains a putative C-X2-C-X15-C-X2-C metal-binding motif was subcloned and overexpressed as TFB-NTD. Purification of the TFB-NTD gene product yields Zn- and Fe-containing forms, which have been characterized by mass spectrometry and UV-visible, electron paramagnetic resonance, and X-ray absorption (XAS) spectroscopies. Only the Zn form of the TFB holoprotein has been (partially) purified, and it has been characterized by XAS. All spectroscopic characteristics are consistent with a nearly tetrahedral MS4 metal-binding site made up of the four cysteine residues in the N-terminal domain. The relatively greater thermal stability of the Zn form suggests that TFB may be a Zn-containing protein involved in archaeal transcription.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5060
    Keywords: Glycine max ; soybean ; breeding methodology ; stress tolerance ; genotype × environment interaction ; yield stability ; productivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Yield data were collected for soybean (Glycine max (L.) Merr.) lines in maturity groups III and IV in 14 environments from 1985 to 1989. The lines in each maturity group were subdivided into three different groups based on the mean yield over all environments, and the genotype × environment interactions were studied for each group. Yield stability of the lines was determined. Effectiveness of selection based on different types of environments was examined. Productivity level of the environment did not influence the relative ranking of the lines. Significant rank correlations occurred between mean yields in most environments and the overall mean yield, but few similarities occurred in the line rankings among individual environments. High-yielding lines contributed a significantly smaller proportion to the genotype × environment interaction than medium- and low-yielding lines. A small proportion of the lines were below or above average stability. Significant correlations occurred among stability, overall mean yield, and mean yield in high- and low-yielding environments. However, few significant correlations occurred between individual environment yields and stability in high- or low-yielding environments. Maturity groups differed in yield and stability relationships. Stability in high- and low-yielding environments did not adequately predict stability for each other.
    Type of Medium: Electronic Resource
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