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  • Artikel: DFG Deutsche Nationallizenzen  (4)
  • Genetic analysis  (2)
  • Lytic enzymes  (1)
  • Multicopy/tandem integration  (1)
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  • Artikel: DFG Deutsche Nationallizenzen  (4)
Materialart
Erscheinungszeitraum
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  • 1
    Digitale Medien
    Digitale Medien
    Adenine and pyrimidine genes of Aspergillus niger and evidence for a seventh linkage group (1989)
    Springer
    Current genetics 16 (1989), S. 307-310 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Aspergillus niger ; Adenine mutants ; Pyrimidine mutants ; Linkage groups ; Genetic analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Mutants of Aspergillus niger requiring adenine and one mutant requiring cytosine were isolated after low-dose mutagenesis and enrichment. In addition we had mutants of two genes involved in the pyrimidine biosynthesis isolated as 5-fluoro-orotic acid-resistant mutants. The fifteen adenine-less mutants could be placed in seven complementation groups. From each group a representative mutant was analyzed in order to determine the linkage group by analysis of the mutants in a heterozygous diploid carrying markers in six linkage groups. AdeF could not be assigned to any one of these linkage groups and proved to be linked to nicB, oliC and cnxC, none of which could be placed in a linkage group. Thus, conclusive evidence was obtained for a seventh linkage group. As pyrA was used as selection marker for transformation, we constructed a pyrA strain with a linked marker which can be used in the genetic analysis of transformants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00422118
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 2
    Digitale Medien
    Digitale Medien
    Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger (1988)
    Springer
    Current genetics 14 (1988), S. 437-443 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Aspergillus niger ; Genetic analysis ; Genetic markers ; Linkage groups ; Master strains
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A start has been made on establishing a collection of Aspergillus niger colour and auxotrophic mutants with an isogenic background for use as a source of genetic markers. All strains have short conidiophores (cspAl ), which makes them easy to handle on test plates. Genetic markers were combined stepwise by somatic recombination. Somatic diploids were obtained at frequencies of 10−6-10−5 with conidiospores collected from a heterokaryon. The haploidization of heterozygous diploids was induced by benomyl. For unlinked markers, the frequency of recombinants varied from 35%–65%. Low frequencies of recombinants were found between markers on a same chromosome, but this was sometimes disturbed by mitotic crossing-over during an early stage of the diploid. Master strains were constructed having markers for six linkage groups.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00521266
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 3
    Digitale Medien
    Digitale Medien
    Isolation of auxotrophic mutants of Aspergillus niger by filtration enrichment and lytic enzymes (1992)
    Springer
    Current genetics 21 (1992), S. 117-120 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Aspergillus niger ; Auxotrophic mutants ; Filtration enrichment ; Novozym enrichment ; Lytic enzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary More than 100 auxotrophic mutants of Aspergillus niger were isolated using filtration enrichment. The mutants obtained in this way were predominantly aminoacid requiring. Hardly any vitamin-deficient mutants were found. The limitations of the method turned out to be due to cross feeding in liquid medium and non-specific loss of ungerminated conidiospores. To circumvent these problems an enrichment method has been used in which conidiospores that germinated on solid medium were selectively killed by the lytic enzyme preparation Novozym 234. We found auxotrophic mutants at high frequencies and several new types of mutants. Optimal conditions for the enrichment procedures have been determined. Essential factors appeared to be segregation of the conidia after mutagenic treatment, in order to obtain synchronization of germination, and the incubation time of the conidia on minimal medium prior to enzymic treatment. Under appropriate conditions Novozym enrichment proved to be very efficient. Depending on the type of mutants desired, one or both procedures can provide an effective method for the enrichment of mutants with a metabolic defect. The Novozym method can be adapted to other fungi and some of the observations that are described may also be of importance to improve other enrichment methods that are based on the selective killing of germinating conidiospores.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318469
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  • 4
    Digitale Medien
    Digitale Medien
    Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene (1985)
    Springer
    Current genetics 9 (1985), S. 361-368 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Transformation of Aspergillus ; Conidial protoplasts ; Multicopy/tandem integration ; Gene amplification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Conidial protoplasts of an A. nidulans amdS deletion strain (MH1277) have been transformed to the AmdS+ phenotype with a plasmid carrying the wild type gene (p3SR2). Optimalisation of transformation and plating conditions now has resulted in frequencies of 300–400 transformants per μg of DNA. Analysis of DNA from AmdS+ transformants of MH1277 showed that transformation had occurred by integration of vector DNA sequences into the genome. In virtually all these transformants multiple copies of the vector were present in a tandemly repeated fashion, not preferentially at the resident, partially deleted amdS gene. It is suggested that the observed integration phenomena are dependent on the genetic background of the A. nidulans strain, used for transformation. A model to explain the tandem type of integration is proposed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00421606
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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