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  • 1
    Electronic Resource
    Electronic Resource
    Binding of tissue-type plasminogen activator to lysine, lysine analogs, and fibrin fragments (1989)
    s.l. : American Chemical Society
    Biochemistry 28 (1989), S. 7318-7325 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00444a026
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  • 2
    Electronic Resource
    Electronic Resource
    Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene (1985)
    Springer
    Current genetics 9 (1985), S. 361-368 
    Details
    ISSN: 1432-0983
    Keywords: Transformation of Aspergillus ; Conidial protoplasts ; Multicopy/tandem integration ; Gene amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Conidial protoplasts of an A. nidulans amdS deletion strain (MH1277) have been transformed to the AmdS+ phenotype with a plasmid carrying the wild type gene (p3SR2). Optimalisation of transformation and plating conditions now has resulted in frequencies of 300–400 transformants per μg of DNA. Analysis of DNA from AmdS+ transformants of MH1277 showed that transformation had occurred by integration of vector DNA sequences into the genome. In virtually all these transformants multiple copies of the vector were present in a tandemly repeated fashion, not preferentially at the resident, partially deleted amdS gene. It is suggested that the observed integration phenomena are dependent on the genetic background of the A. nidulans strain, used for transformation. A model to explain the tandem type of integration is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421606
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of Bacillus subtilis levanase gene in Lactobacillus plantarum and Lactobacillus casei (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 297-303 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Two Lactobacillus-Escherichia coli shuttle vectors, harbouring the levanase gene from Bacillus subtilis under the control of its own promoter (pLPEW1) or behind the E.coli tac promoter (pESIEW2), were constructed. Lactobacillus plantarum showed the same growth characteristics on selective plates and in liquid media containing inulin, after transformation with either pLPEW1 or pESIEW2. L. plantarum transformed with pLPEW1 could be selected on inulin plates, indicating that levanase expression can be used as a food-grade selection system for Lactobacillus. Lactobacillus casei grew faster in inulin-containing medium than L. plantarum after transformation with pESIEW2, but did not grow when harbouring pLPEW1. Inulin-degrading activities of 90 mU/ml were found in culture medium of L. plantarum containing pLPEW1 or pESIEW2, and of 500 mU/ml in medium of L. casei (pESIEW2). Addition of 1 mM isopropyl β-d-thiogalactoside to the culture medium had no effect on growth and levanase expression in L. plantarum (pESIEW2) and L. casei (pESIEW2) strains. Levanase produced by L. casei (pESIEW2) has a size of 75 kDa and 72 kDa, corresponding to that of unprocessed and mature B. subtilis levanase, respectively, suggesting that the protein produced is recognized and processed by a signal peptidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050406
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of Bacillus subtilis levanase gene in Lactobacilus plantarum and Lactobacillus casei (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 297-303 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Two Lactobacillus-Escherichia coli shuttle vectors, harbouring the levanase gene from Bacillus subtilis under the control of its own promoter (pLPEW1) or behind the E.coli tac promoter (pESIEW2), were constructed. Lactobacillus plantarum showed the same growth characteristics on selective plates and in liquid media containing inulin, after transformation with either pLPEW1 or pESIEW2. L. plantarum transformed with pLPEW1 could be selected on inulin plates, indicating that levanase expression can be used as a food-grade selection system for Lactobacillus. Lactobacillus casei grew faster in inulin-containing medium than L. plantarum after transformation with pESIEW2, but did not grow when harbouring pLPEW1. Inulin-degrading activities of 90 mU/ml were found in culture medium of L. plantarum containing pLPEW1 or pESIEW2, and of 500 mU/ml in medium of L. casei (pESIEW2). Addition of 1 mMm isopropyl β-d-thiogalactoside to the culture medium had no effect on growth and levanase expression in L. plantarum (pESIEW2) and L. casei (pESIEW2) strains. Levanase produced by L. casei (pESIEW2) has a size of 75 kDa and 72 kDa, corresponding to that of unprocessed and mature B. subtilis levanase, respectively, suggesting that the protein produced is recognized and processed by a signal peptidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00172828
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  • 5
    Electronic Resource
    Electronic Resource
    A mutant of Escherichia coli K12 deficient in the 5′–3′ exonucleolytic activity of DNA polymerase I (1973)
    Springer
    Molecular genetics and genomics 124 (1973), S. 83-96 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The enzymatic properties of purified DNA polymerase I from a strain of Escherichia coli K12 with a mutation in the polA gene have been studied. The polymerizing activity of the mutant enzyme is similar to that of the enzyme from isogenic wild-type cells, when the activity is measured on exonuclease III treated calf-thymus DNA. Also the 3′–5′ exonucleolytic activity is not significantly different for both enzyme preparations. The 5′–3′ exonucleolytic activity of DNA polymerase I isolated from the mutant strain, however, is much lower than that of wild-type DNA polymerase I. The products formed by the action of the wild-type and the mutant enzyme on nicked circular double-stranded DNA of phage ϕX174′ (RFII DNA) were analysed by sucrose-gradient sedimentation and electron-microscopy. When RFII DNA was incubated with wild-type enzyme 80% of the molecules were converted into linear molecules. All linear molecules were shorter than one phage genome. Only 25% of the molecules were branched. After incubation of RFII DNA with the mutant enzyme 62% of the molecules have become linear. More than 90% of these linear molecules were branched and the majority of them was longer than one phage genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267167
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli (1975)
    Springer
    Molecular genetics and genomics 136 (1975), S. 185-197 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In vitro synthesis of enzymes of the tryptophan (trp) operon of E. coli was studied in an extract prepared from E. coli, which is programmed with purified DNA from trp transducing phages with mutations that effect the expression of the trp genes in various ways. Our results show that control of transcription, i.e. initiation and termination, is similar in vivo and in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334014
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  • 7
    Electronic Resource
    Electronic Resource
    In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli (1975)
    Springer
    Molecular genetics and genomics 136 (1975), S. 215-226 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A protein fraction, called At (=anti termination) factor, has been isolated from extracts of E. coli and partially purified. The At factor stimulates the synthesis in vitro of anthranilate synthetase, an enzyme encoded by two genes of the tryptophan (trp) operon, but has no effect on the synthesis of T7 RNA polymerase and other T7-and T4 coded proteins. The At factor stimulates the synthesis of trp mRNA; it has no effect on the translation of trp mRNA. We conclude that in vitro transcription of the trp operon is positively controlled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334016
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  • 8
    Electronic Resource
    Electronic Resource
    Gene disruption in Lactobacillus plantarum strain 80 by site-specific recombination: isolation of a mutant strain deficient in conjugated bile salt hydrolase activity (1993)
    Springer
    Molecular genetics and genomics 239 (1993), S. 269-272 
    Details
    ISSN: 1617-4623
    Keywords: Lactobacillus ; Site-specific recombination ; Bile salt hydrolase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A chloramphenicol-resistance gene (cml) was introduced into the Lactobacillus plantarum gene encoding conjugated bile acid hydrolasc (cbh) on a ColEl replicon. This plasmid which is nonreplicative in Lactobacillus was used to transform L. plantarum strain 80. A homologous double cross-over recombination event resulted in replacement of the chromosomal cbh gene by the cml-containing cbh gene. The transformants obtained were unable to synthesize active conjugated bile acid hydrolase (Cbh). The Cbh-CmlR phenotype was stably maintained for more than 100 generations under nonselective conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281627
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of the ribosomal protein S1 fromEscherichia coli on the synthesis in vitro of bacterial-, DNA phage- and RNA phage proteins (1977)
    Springer
    Molecular genetics and genomics 153 (1977), S. 75-80 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using an in vitro preparation for protein synthesis, we have studied the effect of the ribosomal protein S1 fromEscherichia coli on the synthesis of the coat protein of the RNA-containing phages Qβ and MS2, on that of an “early” and a ⌈ate” enzyme encoded by the DNA containing phage T7, and on that of anthranilate synthetase, an enzyme encoded by the bacterial tryptophan operon. Our results indicated that for the synthesis of these five proteins the presence of S1 is required. From these results we conclude that S1 is an essential protein for the translation of bacterial and bacteriophage messenger RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01035998
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  • 10
    Electronic Resource
    Electronic Resource
    Formation of poliomyelitis subviral particles in the yeast Saccharomyces cerevisiae (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 907-922 
    Details
    ISSN: 0749-503X
    Keywords: Poliovirus ; subviral particles ; posttranslational cleavage ; heterologous gene expression ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The sequence of the poliovirus genome encoding 3CD (a protease) was transferred to the yeast Saccharomyces cerevisiae on expression vectors with either a constitutive or an inducible promoter. Transformants could only be obtained with vectors carrying the inducible transcription unit. Extracts of induced cells were able to cleave cell-free synthesized P1, the precursor of the poliovirus capsid proteins, into VP0, VP3 and VP1.In yeast cells constitutively expressing P1, induction of 3CD expression resulted in only trace amounts of processed products. Processing could be improved considerably by simultaneous induction of both P1 and 3CD expression. Analysis of extracts of such induced cells revealed the presence of particles that resembled authentic subviral particles.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320100706
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