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  • Articles: DFG German National Licenses  (4)
  • microgravity  (3)
  • Key words Polymerase chain reaction  (1)
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  • Articles: DFG German National Licenses  (4)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Development and application of a heterologous internal standard for polymerase-chain-reaction-based detection of Campylobacter jejuni and Campylobacter coli in foods (1999)
    Springer
    European food research and technology 209 (1999), S. 180-184 
    Details
    ISSN: 1438-2385
    Keywords: Key words Polymerase chain reaction ; Campylobacter ; Internal standard ; Food ; False negatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A heterologous internal standard, termed "mimic", was developed for the polymerase chain reaction (PCR)-based detection of Campylobacter jejuni and Campylobacter coli in food. Mimic was designed to contain a heterologous DNA fragment of plasmid pUC18, flanked by a primer binding site, identical to the bacterial target DNA. Application of mimic in the PCR permitted its co-amplification together with the bacterial DNA with similar efficiency. As the length of the amplified products differed, they were easily detectable by agarose gel electrophoresis. The presence or absence of the mimic PCR product was indicative of the efficacy of the PCR. The use of approximately 60 mimic molecules per reaction was optimal for determining the reliability of the diagnostic PCR assays without decreasing the detection limit. This system for the detection of the two species of Campylobacter was successfully applied in routine food surveillance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050476
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The process of heat and mass transport at the critical point of pure fluids (1995)
    Springer
    International journal of thermophysics 16 (1995), S. 1051-1058 
    Details
    ISSN: 1572-9567
    Keywords: critical phenomena ; D2 Mission ; density relaxation ; microgravity ; SF6 ; temperature relaxation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The effects of fast isentropic temperature propagation, called the “piston effect,” or “critical speeding up,” and slow mass diffusion, called “critical slowing down,” are investigated. A temperature propagation experiment in a spherical cell filled with pure SF6 at critical density was performed during the Second German Spacelab Mission D2 in 1993. The results evidently confirm the presence of the piston effect both in the one-phase region and in the two-phase region. The numerical simulations are in remarkable good quantitative agreement with the experimental results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02081274
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Measurements of the isochoric heatc v at the critical point of SF6 under microgravity: Results of the German Spacelab Mission D2 (1995)
    Springer
    International journal of thermophysics 16 (1995), S. 1033-1049 
    Details
    ISSN: 1572-9567
    Keywords: critical phenomena ; isochoric heat capacity ; microgravity ; sulfur hexafluoride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract During the Second German Spacelab Mission D2 (April 26 to May 6, 1993) the isochoric specific heatc v of SF6 was measured along the critical isochore under microgravity conditions with a newly developed scanning radiation calorimeter. This calorimeter provided the possibility to perform comparable heating and cooling runs with variable ramp rates since the spherical sample cell was heated and cooled only by radiation. During the experimental time of 220 h, 11 heating and cooling runs with different ramp rates were performed in a temperature range ofT–T c=±6 K. ApproachingT c by cooling from the homogeneous one-phase region avoided significant temperature and density gradients in the fluid, which would have distorted the integral measurement ofc v. The inhomogenities introduced by a finite ramp rate were greatly reduced by the fast dynamic temperature propagation (“critical speeding up”). Thec v data achieved with slow cooling runs are in remarkably good agreement with the theoretical prediction more than one order of magnitude closer to the critical point than anyc v measurements done so far. The preliminary value for the critical exponentα is 0.107±0.02, and for the amplitude ratio we obtainedA −/A +=1.94±0.07. In contrast to the cooling runs, the heating runs showed a strong hysteresis ofc v. A comparison to 1g measurements is provided.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02081273
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Isochoric specific heat of sulfur hexafluoride at the critical point: Laboratory results and outline of a spacelab experiment for the d1-mission in 1985 (1986)
    Springer
    International journal of thermophysics 7 (1986), S. 343-356 
    Details
    ISSN: 1572-9567
    Keywords: critical phenomena ; critical exponents ; isochoric specific heat ; scanning ratio calorimeter ; microgravity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The specific heat at constant volume cv shows a weak singularity at the critical point. Renormalization group techniques have been applied, predicting a universal critical behavior which has to be experimentally confirmed for different systems. In this paper an experiment is presented to measure the specific heat of SF6 along the critical isochore (ρc=0.737 g·cm−3), applying a continuous heating method. The results cover a temperature span of −1.5×10−2〈 τ〈1.70×10−2 [τ=(T−T c)/T c] and were strongly affected by gravity effects that emerge in the sample of 1-mm hydrostatic height near the critical point. Using regression analysis, data were fitted with functions of the form c v/R=A × ¦τ¦−α + B for the one-phase state and c v/R=A″ × ¦τ¦−α″ + B″ for the twophase state. Within their error bounds the critical values (α=α″=0.098, A″/A=1.83) represent the measurements for the temperature span 3.5×10−5〈 ¦τ¦〈2.0×10 −3, in good agreement with theoretical predictions. In order to exclude density profiles in the specimen, which are unavoidable in terrestrial experiments due to the high compressibility of fluids at the critical point and the gravity force, a space-qualified scanning ratio calorimeter has been constructed, which will permit long-term cv measurements under microgravity (μ-g) conditions. The experiment will be part of the German Spacelab mission in October 1985. The significant features of the apparatus are briefly sketched.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500160
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    Paper (German National Licenses)
    Fulltext
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