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  • Articles: DFG German National Licenses  (2)
  • Microtubules  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Topographical features of the membrane of Poterioochromonas malhamensis after colchicine and osmotic treatment (1980)
    Springer
    Planta 148 (1980), S. 84-88 
    Details
    ISSN: 1432-2048
    Keywords: Colchicine ; Membrane structure ; Microtubules ; Osmotic treatment ; Plasmalemma ; Poterioochromonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in membrane topography in the flagellate Poterioochromonas malhamensis, as a result of colchicine and osmotic-stress treatments, have been studied using freeze-fracturing and thin sectioning. Ridges, but not rows of intramembrane particles, in the PF-face which denote the position of underlying cortical microtubules, together with the ridge associated with their point of origin (flagellar root fibre 1), dissappear after colchicine or short-term (5 min) osmotic treatments. Cortical microtubules are destroyed as a result of the former, but not the latter treatment. Longer periods in osmoticum allow a recovery of the microtubule — associated membrane ridges. Despite careful isosmotic fixations distinct cross-bridges between microtubules and the plasmalemma were not discernible in thin section.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385446
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of the herbicide oryzalin on cytoskeleton and growth ofFunaria hygrometrica protonemata (1988)
    Springer
    Protoplasma 142 (1988), S. 55-67 
    Details
    ISSN: 1615-6102
    Keywords: Funaria protonema ; Indirect immunofluorescence ; Microtubules ; Oryzalin ; Polar growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protonemata ofFunaria hygrometrica were exposed to the herbicidal MT inhibitor oryzalin. A reduction of the growth rate together with a disturbance of oriented polar growth is observed. Both effects are reversible. Visualization of MT by IFT reveals differential sensitivities of MT. At lower concentrations (⩽10−6 M) only the cytoplasmic MT are depolymerized causing impairment of the migration of the nucleus and the transport of the plastids. Close association of MT with the surface of the plastids is demonstrated. At higher concentrations of oryzalin spindle and phragmoplast MT are affected as well. They are found in unusual orientations and display a variety of aberrant forms like multipolar spindles or the occurrence of several “mini-spindles” within one cell. The mode of action of oryzalin is discussed and the necessity of a continuous network of cytoplasmic MT between nucleus and growing tip for the maintenance of polar growth is emphasized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273226
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    Paper (German National Licenses)
    Fulltext
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