ISSN:
1432-2048
Keywords:
Colchicine
;
Membrane structure
;
Microtubules
;
Osmotic treatment
;
Plasmalemma
;
Poterioochromonas
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Changes in membrane topography in the flagellate Poterioochromonas malhamensis, as a result of colchicine and osmotic-stress treatments, have been studied using freeze-fracturing and thin sectioning. Ridges, but not rows of intramembrane particles, in the PF-face which denote the position of underlying cortical microtubules, together with the ridge associated with their point of origin (flagellar root fibre 1), dissappear after colchicine or short-term (5 min) osmotic treatments. Cortical microtubules are destroyed as a result of the former, but not the latter treatment. Longer periods in osmoticum allow a recovery of the microtubule — associated membrane ridges. Despite careful isosmotic fixations distinct cross-bridges between microtubules and the plasmalemma were not discernible in thin section.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00385446
