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  • Articles: DFG German National Licenses  (5)
  • potato  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning and analysis of four potato tuber mRNAs (1988)
    Springer
    Plant molecular biology 11 (1988), S. 255-269 
    Details
    ISSN: 1573-5028
    Keywords: potato ; patatin ; proteinase inhibitor ; cloning ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tuberization in potato is a complex developmental process involving the expression of a specific set of genes leading to the synthesis of tuber proteins. We here report the cloning and analysis of mRNAs encoding tuber proteins. From a potato tuber cDNA library four different recombinants were isolated which hybridized predominantly with tuber mRNAs. Northern blot hybridization experiments showed that three of them, pPATB2, p303 and p340, can be regarded as tuber-specific while the fourth, p322, hybridizes to tuber and stem mRNA. Hybrid-selected in vitro translation and nucleotide sequence analysis indicate that pPATB2 and p303 represent patatin and the proteinase inhibitor II mRNA respectively. Recombinant p322 represents an mRNA encoding a polypeptide having homology with the soybean Bowman-Birk proteinase inhibitor while p340 represents an mRNA encoding a polypeptide showing homology with the winged bean Kunitz trypsin inhibitor. In total, these four polypeptides constitute approximately 50% of the soluble tuber protein. Using Southern blot analysis of potato DNA we estimate that these mRNAs are encoded by small multigene families.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027383
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Restriction fragment length polymorphism inSolanum spp for the construction of a genetic map ofSolanum tuberosum L.: a preliminary study (1990)
    Springer
    Potato research 33 (1990), S. 171-180 
    Details
    ISSN: 1871-4528
    Keywords: biotin ; potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary When using two restriction enzymes and DNA probes from different sources, high levels of restriction fragment length polymorphism (RFLP) were observed in Southern blot hybridisation experiments, among 6 di(ha)ploid genotypes ofSolanum tuberosum and some closely related species. In 3 F1 sample populations of 14 individuals each, both heterozygosity and segregation of hybridisation patterns were observed. The non-radioactive biotin-dUTP/SA-AP method for DNA detection on Southern blots proved to be satisfactory and reliable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358444
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mapping of resistance to the potato cyst nematode Globodera rostochiensis from the wild potato species Solanum vernei (1996)
    Springer
    Molecular breeding 2 (1996), S. 51-60 
    Details
    ISSN: 1572-9788
    Keywords: BSA ; Globodera rostochiensis ; potato ; RFLP ; SCAR ; Solanum vernei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A population of diploid potato (Solanum tuberosum) was used for the genetic analysis and mapping of a locus for resistance to the potato cyst nematode Globodera rostochiensis, introgressed from the wild potato species Solanum vernei. Resistance tests of 108 genotypes of a F1 population revealed the presence of a single locus with a dominant allele for resistance to G. rostochiensis pathotype Ro1. This locus, designated GroV1, was located on chromosome 5 with RFLP markers. Fine-mapping was performed with RAPD and SCAR markers. The GroV1 locus was found in the same region of the potato genome as the S. tuberosum ssp. andigena H1 nematode resistance locus. Both resistance loci could not excluded to be allelic. The identification of markers flanking the GroV1 locus offers a valuable strategy for marker-assisted selection for introgression of this nematode resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171351
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Phage display of a double-headed proteinase inhibitor: Analysis of the binding domains of potato proteinase inhibitor II (1995)
    Springer
    Molecular breeding 1 (1995), S. 181-191 
    Details
    ISSN: 1572-9788
    Keywords: biopanning ; chymotrypsin ; phage display ; potato ; proteinase inhibitor II ; trypsin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Potato proteinase inhibitor II (PI2) is a serine proteinase inhibitor composed of two domains that are thought to bind independently to proteinases. To determine the activities of each domain separately, various inactive and active domain combinations were constructed by substituting amino acid residues in the active domains by alanines. These derivatives were expressed as soluble protein inEscherichia coli and exposed on M13 phage as fusions to gene 3 in a phagemid system for monovalent phage display. Inactivation of both active domains by Ala residues reduced binding of phage to trypsin and chymotrypsin by 95%. Ten times more phage were bound to proteinases by domain II compared to domain I, while a point mutation (Leu5 → Arg) altered the binding specificity of domain I of PI2 phage from chymotrypsin to trypsin. The mutants were used to show that functional PI2 phage mixed with nonfunctional PI2 phage could be enriched 323 000-fold after three rounds of panning. Thus, these results open up the possibility to use phage display for the selection of engineered PI2 derivatives with improved binding characteristics towards digestive proteinases of plants pests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01249702
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Race specific resistance against Phytophthora infestans in potato is controlled by more genetic factors than only R-genes (1996)
    Springer
    Euphytica 90 (1996), S. 331-336 
    Details
    ISSN: 1573-5060
    Keywords: potato ; Solanum tuberosum ; Phytophthora infestans ; R-genes ; suppressor ; late blight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary For RFLP mapping of R-genes, determining resistance to specific races of Phytophthora infestans in tetraploid potato, it is necessary to develop well segregating populations at the 2x level. During mapping studies, evidence was obtained that more genetic factor(s) are involved in the expression of R-genes than conventionally believed. Two experiments are described in which such an additional genetic factor was suppressing or enhancing the expression of unknown R nand R ifactors. R nand R iappeared to be present in the investigated plant material, containing R4 and R10, or in one of the susceptible crossing parents. In a third experiment, the expression and the segregation of the well known R1 gene was influenced by an additional genetic factor. In that case there were indications for a dominant suppressor. This was established by the selection of susceptible plants carrying a RFLP allele of probe GP21 closely linked to R1. In three of the four F1 populations, resulting from crosses between such susceptible plants and susceptible tester plants, resistnat progenies were found. The resistance appeared to be R1-specific. This clearly indicates that in three of the four investigated susceptible plants, the R1 gene was still present but not expressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027484
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    Paper (German National Licenses)
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