ISSN:
1573-6784
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Saccharomyces cerevisiae lacks most of the depolymerising enzymes required for efficient hydrolysis and utilisation of polysaccharide-rich biomass. To allow extracellular degradation of polysaccharides, genes encoding amylopullulanase (LKA1), pectate lyase (PEL5), polygalacturonase (PEH1), endo-β-1,4-D-glucanase (END1), cellobiohydrolase (CBH1), exo-β-1,3-D-glucanase (EXG1), cellobiase (β-glucosidase; BGL1) and endo-β-D-xylanase (XYN4) were introduced jointly into a laboratory strain of S. cerevisiae. These transformants were able to grow on starch, pectate, cellobiose and to some extent on cellulose (solka-floc and lichenan). These results pave the way for the development of one-step bioconversion processing of plant biomass in the fuel, animal feed, baking and beverage industries. © Rapid Science Ltd. 1998
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008829129516
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