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  • Articles: DFG German National Licenses  (21)
  • 11
    Electronic Resource
    Electronic Resource
    Existence of various structural zones in keratinous tissues revealed by X-ray microdiffraction (1999)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 6 (1999), S. 1021-1030 
    Details
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: Keratinous tissues play two major roles in the adaptation of vertebrates to their environment: a strong mechanical support and a chemical barrier. In order to determine whether these properties may originate from different zones in the tissues, microdiffraction experiments on the micrometre scale have been carried out on feather shaft, horse and human hair, and porcupine quill samples. The existence of several structural layers has been revealed in all the tissues, some corresponding to highly ordered α- or β-type keratin and the others to more or less amorphous keratin. The existence of lipid granules has also been evidenced, mainly in the outer layers. This study shows one of the possibilities which are now offered by third-generation synchrotron sources for the structural microanalysis of biological tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0909049599004537
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  • 12
    Electronic Resource
    Electronic Resource
    Investigation of the Variation in Orientation and Crystallinity in Poly(ethylene terephthalate) Containers Using Microfocus X-ray Diffraction (1997)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 4 (1997), S. 223-227 
    Details
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: The microfocus X-ray beamline at the European Synchrotron Radiation Facility has been used to investigate the variation in molecular orientation and crystallinity in the wall of a container fabricated from poly(ethylene terephthalate). Two-dimensional wide-angle X-ray scattering patterns were recorded and displayed in real time as the specimen was tracked across the incident X-ray beam enabling the measurement of textural changes to be made with a spatial resolution of 2̃ μm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0909049597004512
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  • 13
    Electronic Resource
    Electronic Resource
    The Use of a Scanning X-ray Microprobe for Simultaneous XRF/XRD Studies of Fly-Ash Particles (1997)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 4 (1997), S. 228-235 
    Details
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: With the opening of the first real `third-generation' synchrotron source in Grenoble, in fall 1994, X-ray sources of unprecedented brilliances and qualities became available to the scientific community. Different X-ray analytical techniques could now be applied on a level that was unimaginable only a decade ago. Here are some preliminary results from an experiment where different analytical techniques have been applied on a micrometer level carried out at the most powerful synchrotron microbeam currently available in the world, the microfocus beamline (BL1) at ESRF. This beamline can now provide micrometer-sized X-ray beams with a flux density up to 1010 photons μm−2 at an energy of 13 keV and with a bandwidth of 10–4. In this experiment, X-ray diffraction and X-ray fluorescence have been combined in order to obtain a precise and comprehensive micro-analytical description of micrometer-sized fly ash particles. These types of particles are heavily inhomogeneous with a very irregular shape that makes them inaccessible to conventional micro-analysis. The experiment was performed in a scanning mode and two-dimensional images of different analytical information were reconstructed from the data recorded during the scan. The major features and limitations of this micro-analytical technique will be outlined and different examples on how the analytical information can be used for generating two-dimensional images of the sample will be demonstrated and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0909049597000344
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    Paper (German National Licenses)
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  • 14
    Electronic Resource
    Electronic Resource
    Microfocus X-ray Diffraction of Spherulites of Poly-3-hydroxybutyrate (1995)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 2 (1995), S. 308-312 
    Details
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: The microfocus X-ray beamline at the European Synchrotron Radiation Facility has been used to investigate the variation in molecular orientation and crystallinity in spherulites of the organic polymer poly-3-hydroxybutyrate (PHB). This is the first report of the correlation of optical and X-ray measurements on spherulitic polymer films where X-ray diffraction patterns have been recorded and displayed continuously in real time while the specimen was tracked in steps of 10 μm across an incident X-ray beam with a diameter as small as 10 μm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0909049595009769
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  • 15
    Electronic Resource
    Electronic Resource
    Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens — mediated gene transfer (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 246-252 
    Details
    ISSN: 1432-2242
    Keywords: Transformation ; Pea ; Pisum sativum ; Agrobacterium tumefaciens ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A transformation system that allows regeneration of transgenic pea plants from calli selected for antibiotic resistance was developed. Explants from axenic shoot cultures and seedling epicotyls were cocultivated with nononcogenic Agrobacterium tumefaciens strains, and transformed callus could be selected on callus-inducing media containing either 15 mg/l hygromycin or 75 mg/l kanamycin. After several passages on regeneration medium, shoot organogenesis could be reproducibly induced on hygromycin-resistant calli, but not on the calli selected for kanamycin resistance. Regenerated shoots could subsequently be rooted and transferred into the greenhouse. In addition, the effects of different callus-inducing and growth media on organogenesis were investigated. The transformation of the calli and regenerated plants was confirmed by DNA analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224394
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    Paper (German National Licenses)
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  • 16
    Electronic Resource
    Electronic Resource
    Inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 443-450 
    Details
    ISSN: 1432-2242
    Keywords: Transformation ; Pisum sativum ; Agrobacterium tumefaciens ; Regeneration ; Transgenic plants ; Progeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An analysis of the progeny of primary transgenic pea plants in terms of transmission of the transferred DNA, fertility and morphology is presented. A transformation system developed for pea that allows the regeneration of fertile transgenic pea plants from calli selected for antibiotic resistance was used. Expiants from axenic shoot cultures were co-cultivated with a nononcogenic Agrobacterium tumefaciens strain carrying a gene encoding hygromycin phosphotransferase as selectable marker, and transformed callus could be selected on callus-inducing media containing 15 mg/l hygromycin. After several passages on regeneration medium, shoot organogenesis could be reproducibly induced on the hygromycin resistant calli, and the regenerated shoots could subsequently be rooted and transferred to the greenhouse, where they proceeded to flower and set seed. The transmission of the introduced gene into the progeny of the regenerated transgenic plants was studied over two generations, and stable transmission was shown to take place. The transgenic nature of the calli and regenerated plants and their progeny was confirmed by DNA and RNA analysis. The DNA and ploidy levels of the progeny plants and primary regenerants were studied by chromosome analysis, and the offspring of the primary transformants were evaluated morphologically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229505
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    Paper (German National Licenses)
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  • 17
    Electronic Resource
    Electronic Resource
    Immunoglobulin levels in saliva in individuals with selective IgA deficiency: Compensatory IgM secretion and its correlation with HLA and susceptibility to infections (1989)
    Springer
    Journal of clinical immunology 9 (1989), S. 279-286 
    Details
    ISSN: 1573-2592
    Keywords: IgA deficiency ; saliva antibodies ; HLA ; infections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Total levels of IgA, IgM, and IgG were measured in unstimulated whole saliva and serum from 63 individuals with selective IgA deficiency. Values were compared with the incidence of upper respiratory tract infections, antibiotic treatments (necessitated by upper respiratory tract infections), and HLA antigens. A statistically significant increase in salivary IgM and IgG levels was noted in individuals with selective IgA deficiency compared to healthy normal individuals. Healthy individuals with selective IgA deficiency did not have increased concentrations of salivary IgM compared to infectious-prone patients. Nor was there any correlation found between proneness to infections and HLA antigens or between salivary IgM or IgG levels and HLA antigens in this patient material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00918659
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    Paper (German National Licenses)
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  • 18
    Electronic Resource
    Electronic Resource
    Oral and intestinal microflora in individuals with different immunoglobulin deficiencies (1990)
    Springer
    European journal of clinical microbiology & infectious diseases 9 (1990), S. 631-633 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01967224
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    Paper (German National Licenses)
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  • 19
    Electronic Resource
    Electronic Resource
    Microbeam technique for energy-dispersive x-ray fluorescence (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    X-Ray Spectrometry 18 (1989), S. 109-112 
    Details
    ISSN: 0049-8246
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: By using the total reflecting properties of x-rays inside glass capillary tubes, intense fine or microbeams of x-rays can be obtained. In combination with conventional diffraction x-ray tubes, microbeams with a diameter of a few micrometres and with an intensity sufficient for trace element analysis can be achieved. The basic physics behind the capillary technique is reviewed and an energy-dispersive x-ray fluorescence microbeam spectrometer for trace element analysis is described. Examples of applications of the microbeam spectrometer are given and the future perspectives of the capillary technique are discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/xrs.1300180307
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    Paper (German National Licenses)
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  • 20
    Electronic Resource
    Electronic Resource
    Elemental mapping of bone tissues by the use of capillary focused XRFPresented at the European Conference on EDXRS, Myconos, Greece, 30 May-6 June 1992. (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    X-Ray Spectrometry 22 (1993), S. 229-233 
    Details
    ISSN: 0049-8246
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A description of the x-ray microbeam spectrometer at Chalmers University of Technology is given with particular emphasis on the mapping and imaging features. The application of the microbeam technique to the analysis of bone specimens is also described.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/xrs.1300220411
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