ISSN:
1013-9826
Quelle:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Thema:
Maschinenbau
Notizen:
To obtain an enhanced population of cardiomyocytes from differentiating mouseembryonic stem (ES) cells, we confirmed the role of noggin treatment during the cardiacdifferentiation of mouse ES cells. ES cells were cultured in ES medium containing both noggin andLIF for 3 days on the mouse embryonic fibroblast feeder layer, followed by dissociated andsuspension culture without LIF to form the embryoid body (EB). The next day, noggin waseliminated and EBs were cultured continuously. Noggin treated ES cells showed a relatively rapidincrease of cardiac marker genes, while the vehicle (PBS) treated group showed no significantcardiac marker expression at 4 days after the EB formation. Furthermore, Noggin treated ES cellsshowed 68.00±9.16% spontaneous beating EBs at 12 days after the EB formation. To develop amore efficient cardiomyocyte differentiation method, we tested several known cardiogenic reagents(ascorbic acid, 5’-Azacytidine, LiCl, oxytocin, FGF2 and PDGF-BB) after noggin induction or wecultured noggin treated ES cells on various extracellular matrixes (collagen, fibronectin andMatrigel). Quantitative RT-PCR and immunocytochemistry results showed a significantly increasedcardiac differentiation rate in the FGF2 treated group. Differentiation on the collagen extracellularmatrix (ECM) could slightly increase the cardiac differentiation efficiency. These results show thepossibilities for the establishment of selective differentiation conditions for the cardiacdifferentiation of mouse ES cells
Materialart:
Digitale Medien
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.25.pdf
Permalink
