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  • Artikel: DFG Deutsche Nationallizenzen  (26)
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  • Artikel: DFG Deutsche Nationallizenzen  (26)
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  • 1
    Digitale Medien
    Digitale Medien
    Fungal Genetics (1970)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 4 (1970), S. 347-372 
    Details
    ISSN: 0066-4197
    Quelle: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1146/annurev.ge.04.120170.002023
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  • 2
    Digitale Medien
    Digitale Medien
    Controlling Elements in Maize (1974)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 8 (1974), S. 15-50 
    Details
    ISSN: 0066-4197
    Quelle: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1146/annurev.ge.08.120174.000311
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  • 3
    Digitale Medien
    Digitale Medien
    The Biochemistry of Genetic Factors (1959)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Biochemistry 28 (1959), S. 343-364 
    Details
    ISSN: 0066-4154
    Quelle: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Thema: Chemie und Pharmazie , Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1146/annurev.bi.28.070159.002015
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  • 4
    Digitale Medien
    Digitale Medien
    Comparison and cross-species expression of the acetyl-CoA synthetase genes of the ascomycete fungi, Aspergillus nidulans and Neurospora crassa (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 4 (1990), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: The genes encoding the acetate-inducible enzyme acetyl-coenzyme A synthetase from Neurospora crassa and Aspergillus nidulans (acu-5 and facA, respectively) have been cloned and their sequences compared. The predicted amino acid sequence of the Aspergillus enzyme has 670 amino acid residues and that of the Neurospora enzyme either 626 or 606 residues, depending upon which of the two possible initiation codons is used. The amino acid sequences following the second alternative AUG show 86% homology between the two species; the extended N-terminal sequences show no homology. The Neurospora protein is characterized by the appearance of the S(T)PXX sequence motif where the amino acid homologies break down. The codon usage is biased in both genes, with a marked deficiency, especially in Neurospora, of codons with A in the third position.The facA transcribed sequence contains six introns: one in the long leader sequence, one in the 5′ coding sequence not homologous with acu-5, and four within the sequence that is largely similar to that of acu-5. Only one intron, corresponding in size and position to the furthest downstream of the facA introns, is found in acu-5. The evolution of introns during the divergence of these two Ascomycete fungi is discussed. Each of the two genes has been transferred by transformation into the other species. Each species is evidently able to splice out the other's introns. Most transformants have normal acetate-induction of acetyl-CoA synthetase, emptying that the two genes respond to transcriptional control signals common to both species, in spite of the striking divergence of their 5′ ends.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1990.tb00611.x
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  • 5
    Digitale Medien
    Digitale Medien
    Molecular cloning, identification and ranscriptional analysis of genes involved in acetate utilization in Neurospora crassa (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Four Neurospora crassa genomic clones have been selected as hybridizing much more strongly to labeled mRNA isolated from acetate-grown mycelium than to mRNA from sucrose-grown mycelium. Hybridization of restriction fragments with acetate-specific mRNA or cDNA has been used to delimit the transcribed region(s) of each clone. The transcription of all four clones is strongly induced by transfer of growing mycelium from sucrose to acetate as sole carbon source. In wild-type mycelium, mRNAs corresponding to the four clones reach maximum levels after four hours of induction. They accumulate more rapidly and reach higher levels in an acetate non-utilizing mutant, acu-7, which has been found to overproduce enzymes of the glyoxylate cycle and to have a partial block in the TCA cycle. Molecular transformation of a Neurosporaacu-5 mutant and of an Aspergillus nidulans acuE mutant by DNA of clone 2 and clone 1, respectively, strongly suggests that clone 2 codes for acetyl-coenzyme A synthetase and that clone 1 codes for malate synthase. The transcribed segments of clones 1 and 2each hybridize to corresponding clones from Aspergillusnidulans (R. A. Sandeman and M. J. Hynes, personal communication).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1988.tb00068.x
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  • 6
    Digitale Medien
    Digitale Medien
    Calcium measurement in living filamentous fungi expressing codon-optimized aequorin (2004)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 52 (2004), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Calcium signalling is little understood in filamentous fungi largely because easy and routine methods for calcium measurement in living hyphae have previously been unavailable. We have developed the recombinant aequorin method for this purpose. High levels of aequorin expression were obtained in Neurospora crassa, Aspergillus niger and Aspergillus awamori by codon optimization of the aequorin gene. Three external stimuli (mechanical perturbation, hypo-osmotic shock and high external calcium) were found transiently to increase [Ca2+]c. Each of the calcium signatures associated with these physico-chemical treatments was unique, suggesting the involvement of three distinct calcium-mediated signal transduction pathways. The fungal calcium channel blocker KP4 inhibited the [Ca2+]c responses to hypo-osmotic shock and high external calcium, but not to mechanical perturbation. The divalent cation chelator BAPTA inhibited [Ca2+]c responses to mechanical perturbation and hypo-osmotic shock. The calcium agonists A23187 and cyclopiazonic acid increased [Ca2+]c levels.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.2004.04066.x
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  • 7
    Digitale Medien
    Digitale Medien
    Transaminases in Neurospora crassa (1951)
    [s.l.] : Nature Publishing Group
    Nature 168 (1951), S. 957-958 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Mycelium of the wild-type strain E5297a was grown for three days at 25 in 'minimal' Fries No. 3 medium, and cell-free extracts were obtained by grinding with phosphate buffer and powdered glass followed by centrifugation as described in detail elsewhere8. The extracts, which were free of large ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/168957b0
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  • 8
    Digitale Medien
    Digitale Medien
    Mutable Genes in the Light of Callan's Hypothesis of Serially Repeated Gene Copies (1967)
    [s.l.] : Nature Publishing Group
    Nature 215 (1967), S. 864-866 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] To explain the almost continuously graded variation seen in derivatives of mutable genes Brink5,6 has proposed that such variation is caused by different numbers of repeated, probably identical self-replicating units associated with the unstable locus. It has not been clear, however, how the ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/215864a0
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  • 9
    Digitale Medien
    Digitale Medien
    The killing fields (1995)
    [s.l.] : Nature Publishing Group
    Nature 377 (1995), S. 297-297 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] SUCCESSFUL plant breeding depends on a pool of genetic variation to which selection can be applied. But by producing highly productive varieties that take over large sectors of the market, its natural tendency is to drive much of that variation out of existence. At the same time, the reservoir of ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/377297a0
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  • 10
    Digitale Medien
    Digitale Medien
    Reductionism should be clarified, not dismissed (2000)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 406 (2000), S. 343-343 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Sir John Ziman, reviewing Norman Levitt's book Prometheus Bedeviled (Nature 404, 811; 2000) calls reductionism a “busted flush”. But what is reductionism? Molecular biologists in particular tend to be accused of it, ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/35019178
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