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  • Electronic Resource  (16)
  • 1970-1974  (16)
  • 1972  (16)
Source
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  • Electronic Resource  (16)
Years
  • 1970-1974  (16)
Year
Keywords
  • 11
    Electronic Resource
    Electronic Resource
    A cytochemical study of the calcium-activated adenosinetriphosphatase in hamster adrenal medulla: its occurrence in the golgi region of chromaffin cells (1972)
    Springer
    Histochemistry and cell biology 29 (1972), S. 16-27 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several different fixation procedures and incubation media were used in order to demonstrate the ultrastructural localisation of Ca2+-activated adenosinetriphosphatase (ATPase) in the hamster adrenal medulla. Fixation by perfusion with 2.5% glutaraldehyde gave the best preservation of fine structure without markedly inhibiting the enzymic activity. The localisation of Ca2+-activated ATPase was different from that of Mg2+-activated ATPase: the Mg2+-dependent enzyme was confined to plasma membranes. Ca2+-dependent ATPase also occurred on the plasma membranes of neurons and of some chromaffin cells, but the most prominent site of this enzyme was in the Golgi apparatus of chromaffin cells. Most of the reaction product was localised between Golgi lamellae, but some was found in Golgi vesicles and in prosecretory granules. The nucleus, mature chromaffin granules, roughsurfaced endoplasmic reticulum and mitochondria were usually free of reaction product. Rarely, some precipitate was found in the matrix of mitochondria and in lysosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305697
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  • 12
    Electronic Resource
    Electronic Resource
    The interference of L-ascorbic acid with a standard histochemical azo dye coupling procedure (1972)
    Springer
    Histochemistry and cell biology 30 (1972), S. 273-276 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The reducing agent, L-ascorhic acid, has been shown to interfere with the chromogenesis of free Napthol AS D, and the diazonium salt, fast red violet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277598
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    Paper (German National Licenses)
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  • 13
    Electronic Resource
    Electronic Resource
    Ruthenium red staining of the hamster adrenal medulla (1972)
    Springer
    Histochemistry and cell biology 32 (1972), S. 213-219 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ruthenium red “staining” of the surface coats was studied in the adrenal medullary cells of golden hamster. Both immersion and perfusion fixation was used with the ruthenium red containing fixative, however, only the perfusion fixation gave positive results. A rather thick electron dense ruthenium red positive layer was found on the plasma membrane of the endothelial cells, around the capillaries in the basal lamina, in the basement membrane of the chromaffin cells as well as on the apical and lateral cell surfaces of the adrenomedullary cells. Coated pits and coated vesicles usually showed an intensive ruthenium red staining, but the other cell components in the cytoplasm did not. On the basis of these observations author suggests that the ruthenium red positive material corresponds to acidic mucopolysaccharides in the hamster adrenal medulla, and its wide-ranging occurrence is indicative of its significance in the secretion process of catecholamines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306029
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  • 14
    Electronic Resource
    Electronic Resource
    Topography of rubrospinal units in the cat (1972)
    Springer
    Experimental brain research 14 (1972), S. 363-371 
    Details
    ISSN: 1432-1106
    Keywords: Rubrospinal cells ; Unit recording ; Topographical organization ; Conduction velocity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mapping of cells at the origin of the rubrospinal tract was conducted in the cat. 1. Rubrospinal neurons sending efferents to cervico-thoracic segments of the spinal cord are located in the dorso-medial part of the nucleus. These neurons are especially medial in the caudal planes and especially dorsal in the rostral planes. Neurons with efferents terminating at the level of lumbo-sacral segments of the cord occupy the ventro-lateral part of the nucleus. These neurons are especially lateral in the caudal planes and especially ventral in the rostral planes. The limit between these two cell populations is clear in the caudal and middle thirds of the nucleus but considerable overlap is seen in the rostral third. These results agree with the anatomical findings of Pompeiano and Brodal (1957). 2. For the population of lumbar neurons the conduction velocities ranged from 31 m/sec to more than 120 m/sec with a mean of 85 m/sec. 3. Rubrospinal cells are found throughout the nucleus. The most caudal planes are essentially composed of cells with rapidly conducting fibers whereas in the middle and rostral planes a cell population with increasingly slower conducting fibers appears. The results of the present study are discussed in relation to classical data on the magnocellular and parvocellular divisions of the red nucleus. 2.The third author acknowledge the personal support of the Medical Research Council of Canada.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235033
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  • 15
    Electronic Resource
    Electronic Resource
    Ultrastructural studies on the adrenal medulla of golden hamster: Origin and fate of secretory granules (1972)
    Springer
    Cell & tissue research 124 (1972), S. 367-386 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal medulla ; Golden hamster ; Secretion ; Lysosomes ; Coated vesicles ; Fixation for electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several different fixatives were used in order to obtain the best preservation of fine structure in the chromaffin cells of hamster adrenal medulla. The best fixative for both immersion-fixation and perfusion-fixation contained glutaraldehyde (2.5%) and formaldehyde (4%). After fixation by immersion of the gland, both “dark” and “light” cells are found, but glands fixed by perfusion contain a homogeneous population of “light” cells, which were very well preserved. The plasma membrane along the “free” surface of chromaffin cells showed a large number of omega-shaped invaginations that usually contained a dense core or fibre-like material; the extracellular dense cores were very similar to those of intact secretory granules. Rarely, the extracellular dense cores were very large and resembled the contents of a secondary lysosome. Several coated pits were found on the inner surface of each omega-shaped invagination. A prominent Golgi zone, containing many coated vesicles, is typical of these chromaffin cells. The coated vesicles are of two kinds, one with and one without electron-dense contents. Coated vesicles were frequently found in close contact with, or fused with, pro-secretory granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00355037
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  • 16
    Electronic Resource
    Electronic Resource
    Alterations in the distribution of yellow fluorescing rabbit pinealocytes produced by p-chlorophenylalanine and different conditions of illumination (1972)
    Springer
    Journal of neural transmission 33 (1972), S. 91-111 
    Details
    ISSN: 1435-1463
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fluorescence Microscopy Changes in the density of yellow autofluorescent and serotonincontaining pinealocytes in the rabbit pineal gland have been studied under different experimental conditions such as p-chlorophenylalanine, environmental lighting and permanent darkness, using fluorescence microscopy. The yellow autofluorescent pinealocytes (Type II), particularly present in a circumscript area of the organ, increased in number during treatment of the animals with pCPA as well as during the night under environmental lighting conditions. This increase probably accurred at the cost of the decrease in number of serotonin-containing pinealocytes (Type I) in the same area, originally present. Moreover it could be demonstrated that under environmental lighting conditions both, the number of Type I and Type II cells, showed a day and night rhythm. During continuous darkness the circadian rhythm in the serotonin content of the Type I cells persists. Evidently, this rhythm is not controlled by exogenous environmental lighting conditions but endogenously. In contrast to the persisting circadian serotonin rhythm, no such fluctuations could be observed in the yellow autofluorescing compound in the Type II cells. Light Microscopy Evidence is presented indicating that the yellow autofluorescent compound, present in the rabbit pinealocytes, is identical with a protein containing much tryptophan. In the rabbit pineal gland three different patterns of intramural blood vessels can be distinguished which are, respectively, situated in (1) a thin cortex, (2) a medulla, and (3) a transition area of the gland situated between the cortex and the medulla, containing the Type I and Type II pinealocytes of population B (see introduction). These studies revealed that the Type I and Type II pinealocytes of population B in close topographical contact with the intrapineal capillary system of the transition area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01260899
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    Paper (German National Licenses)
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