Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Electronic Resource  (19)
  • 1995-1999  (19)
  • 1997  (9)
  • 1995  (10)
  • 11
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  We devised a new microfluorometric method for determining the ploidy of megakaryocytes identified immunologically in bone marrow smears. The smears were immunostained by incubation with mouse monoclonal anti-glycoproteins (GP) IIb antibodies, followed by fluorescein isothiocyanate-conjugated goat anti-mouse IgG antibodies. They were then stained with 4′,6-diamidino-2-phenylindole (DAPI). Megakaryocytes were identified by their GPIIb immunofluorescence using a microfluorometer and, after the filters were changed, their DNA content was assayed by measuring the intensity of DAPI fluorescence. This intensity was shown to be proportional to the DNA content when the aperture of the objective lens was reduced. We compared these results with those obtained when megakaryocytes were identified morphologically, using DAPI staining after Wright-Giemsa destaining. In all 12 normal controls, the ploidy peaks were shown to be 16N by both methods, and the mean ploidy detected by the immunological method was only reduced 0.961 times relative to the estimate from the morphological method. In contrast, in eight myelodysplastic syndrome (MDS) patients, the ploidy peaks were either 8N or 4N and the mean was reduced by 0.906 times (P=0.018). Thus we could immunologically identify small megakaryocytes which we could not identify morphologically. Therefore, this method is useful for measuring megakaryocytic ploidy, especially in the pathological megakaryocytes of MDS patients.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 12
    ISSN: 1432-1084
    Keywords: Angiomyolipoma ; Tumor thrombus ; “Thread-and-streaks” sign ; Inferior vena cava ; MR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The MR findings in a case of tumor extension into an inferior vena cava from a right renal angiomyolipoma are reported. The flow void demonstrated within the intracaval tumor thrombus on T1-weighted images was consistent with the so-called thread-and-streaks sign.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 16 (1997), S. 1084-1085 
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Abstracts are not published in this journal
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 14
    ISSN: 1617-4623
    Keywords: Key words Gene transfer ; NADH: ubiquinone oxidoreductase ; Liverwort ; Signal peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nad7 gene, encoding subunit 7 of NADH dehydrogenase, is mitochondrially encoded in seed plants. In the liverwort, Marchantia polymorpha, only a pseudogene is located in the mitochondrial genome. We have now identified the functional nad7 gene copy in the nuclear genome of Marchantia, coding for a polypeptide of 468 amino acids. The nuclear-encoded nad7 has lost the two group II introns present in the mitochondrial pseudogene copy. Instead, a typical nuclear intron is found to split an exon encoding the presumptive mitochondrial targeting signal peptide and the mature subunit 7 of NADH dehydrogenase. These results suggest that RNA-mediated gene transfer from the mitochondrial into the nuclear genome occurs not only in seed plants but also in bryophytes.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 30 (1995), S. 3333-3338 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Solid products were prepared from mixtures of zirconyl carbonate and sulphuric acid solution under thermal hydrolysis at 240°C. These crystalline products and their formation mechanism were investigated by evaluating the amount of products, and by X-ray diffraction analysis and TEM observation. The solid products precipitated were zirconium oxide sulphates (ZOS, PZOS, ZrOSO4·H2O) and m-ZrO2. The area and thickness of the fine plate-like crystals of ZOS increased with increasing concentration of the solution and hydrothermal treatment time. For thin starting solutions, the precipitation velocity and the amount of PZOS increased. For thick starting solutions, those of ZOS increased. For 0.5 mol/l−1 ZrOSO4 solution (ZS0.5) from the thin solution, PZOS crystallized early and m-ZrO2 crystallized by releasing sulphuric acid ions in PZOS with treatment time. For ZS2.5 from the thick solution, ZrOSO4·H2O was crystallized by increasing the proportion of sulphuric acid ions to zirconium ions in the solution, which was caused by ZOS precipitation.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 32 (1997), S. 1789-1794 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Several kinds of metastable compounds, pseudo zirconium oxide sulphates (PZOS) with a chemical composition of Zr3O5SO4·nH2O, were previously synthesized by the thermal hydrolysis of solutions containing zirconium sulphate at 200 or 240°C. The obtained PZOS samples were again hydrothermally treated in different sulphuric acid solutions (〈1.0 mol l-1) at 240°C, and their hydrothermal decomposition behaviour was investigated by TEM observation. The PZOS samples mostly crystallized to plate-like zirconium oxide sulphate (ZOS) in the concentrated sulphuric acid solution (〉0.5 mol l-1), but long-whiskered monoclinic ZrO2 crystals grew with decomposition of the PZOS samples obtained from the starting mixtures with added Zr(OH)4 when rapidly heated to the hydrothermal treatment temperatures. It was found that many ultrafine monoclinic ZrO2 crystals were simultaneously formed during the hydrothermal preparation of the PZOS samples, and during the following hydrothermal decomposition of the PZOS samples, the whiskered crystals of monoclinic ZrO2 grew with the consumption of PZOS from the coexisting ultrafine monoclinic ZrO2 particles which act as seed crystals.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 17
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The features of time-differential perturbed angular correlation (TDPAC) in studies on local chemical structures around impurity atoms are described in comparison with those of Mössbauer emission spectroscopy with four typical examples of recent applications to metal oxide systems, namely,99Rh→99Ru in α-Fe2O3 and YBa2Cu3O7−δ and117Cd→117In in MO (M=Mn, Co, Ni) and BaRu2/3Cd1/3O3.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 18
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract We established “Multitracer”, a new versatile radiotracer technique, for simultaneous tracing of a number of elements in various chemical, environmental, and biological systems. Metal foil targets (typically Au, Ag, Ge, Cu and Fe) are irradiated with C, N, or O ions accelerated up to 135 MeV/nucleon by RIKEN Ring Cyclotron. Radiochemical procedures have been developed to remove the target material leaving the nuclides as Multitracer solutions containing various radionuclides of Be, Na, Mg, K, Ca, Sc, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Se, Br, Rb, Sr, Y, Zr, Nb, Mo, Tc, Ru, Rh, Pd, Ag, Cd, In, Sn, Te, I, Ba, Ce, Pm, Eu, Gd, Tb, Tm, Yb, Lu, Hf, W, Re, Os, Ir, Pt, and Hg. Multitracers enable efficient tracing of a number of elements, and comparison of their behavior under strictly identical experimental conditions. Such features will be demonstrated by means of an example of application to a model experiment for the study of removal mechanism of various elements from the ocean.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 195 (1995), S. 305-313 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Transport and distribution of metal ions in rice and soybean plants were studied using multitracers produced by irradiating an Au target by 135 MeV/nucleon12C,14N, or16O ions accelerated by RIKEN Ring Cyclotron. The multitracer consisted of radioisotopes of the following elements: Be, Na, Sc, Mn, Fe, Co, Zn, Se, Rb, Sr, Y, Zr, Nb, Ag, Te, Ba, Ce, Pm, Eu, Gd, Tb, Tm, Yb, Lu, Hf, Ir, and Pt. Rice and soybean plants were grown in a nutrient solution and also on soil containing a multitracer. γ-ray spectroscopy of different parts of the plants showed that all the elements were more or less taken up by roots, while appreciable transport to leaves and seeds was observed only for Mn, Zn, Se, Rb, Sr, and Ba.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...