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  • Electronic Resource  (3)
  • 1995-1999  (3)
  • 1975-1979
  • 1998  (3)
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  • Electronic Resource  (3)
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  • 1995-1999  (3)
  • 1975-1979
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  • 1
    Electronic Resource
    Electronic Resource
    Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1044-1049 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa ; Stripe resistance gene ; Genetic map ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We used graphical genotyping and linkage analyses with molecular markers to determine the chromosomal location of the rice stripe disease resistance gene, Stv-b i . The stripe resistance gene from the indica rice (Oryza sativa) cv ‘Modan’ was introgressed into several Japanese rice varieties. We found 4 RFLP markers in ‘Modan’, five susceptible parental rice varieties (‘Norin No. 8’, ‘Sachihikari’, ‘Kanto No. 98’, ‘Hokuriku No.103’ and ‘Koganebare’) and four resistant progeny varieties (‘St. No. 1’, ‘Aichi No. 6’, ‘Aoisora’ and ‘Asanohikari’). Graphical genotyping of the resistant progeny revealed a chromosomal segment ascribable to ‘Modan’ and associated with stripe resistance. The chromosomal segment from ‘Modan’ was located at 35.85 cM on chromosome 11. Linkage analysis using 120 F2 individuals from a cross between ‘Koshihikari’ (susceptible) and ‘Asanohikari’ (resistant) revealed another 8 RFLP markers in the same chromosome. We performed a bioassay for rice stripe resistance in F3 lines of the F2 individuals using infective small brown planthoppers and identified an 1.8-cM segment harboring the rice stripe disease resistance gene, Stv-b i , between XNpb220 and XNpb257/ XNpb254. Furthermore, Stv-b i was linked by 0.0 cM to a RFLP marker, ST10, which was developed on the basis of the results of RAPD analysis. These DNA markers near the Stv-b i locus may be useful in marker-assisted selection and map-based cloning of the Stv-b i gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050837
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Slight difference in primary amino acid sequence of p17 matrix protein of HIV-1 exerts profound influence on its antigenicity (1998)
    Springer
    Archives of virology 143 (1998), S. 881-890 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  HIV-1 p17 antigen has been studied for its biological significance in vitro as well as its immunological roles in vivo. By immunological approach of antibody-binding to HIV-1 p17 antigens of several subtypes in combination with computerized analysis of those tertial structures, it became evident that, irrelevant of similarity of linear amino acid sequence of different HIV-1 subtypes, a few amino acid substitutions close to or distant from specified epitope(s) affected their tertial structure resulting in change in ability of its binding to selected antibody. ELISA employing two monoclonal antibodies, A144 and C415, could detect p17 of subtypes A and B, but not of subtypes C, D, and E. Since the epitope site corresponding to A144 has been reported to be important for biological activity of p17 of HIV-1, change in tertial structure around this epitope may explain some difference in biology of HIV-1, such as infectivity of subtypes B and E.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050339
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Preparation of Ce3+-Doped Inorganic-Organic Hybrid Materials Using Functionalized Silanes (1998)
    Springer
    Journal of sol gel science and technology 13 (1998), S. 587-591 
    Details
    ISSN: 1573-4846
    Keywords: functionalized silane ; ligand ; cerium ; inorganic-organic hybrid material
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Ce3+-doped, inorganic-organic hybrid monoliths were prepared from Si(OCH3)4, CH3 Si(OCH3)3, CeCl3 and various functionalized silanes (FSs) such as amino- (APTM), glycidyloxy- (GPTM), trifluoro- (TFTM) and chloro-(CPTM) silanes at 60°C by the sol-gel process. The functional groups of silane coupling agents coordinated with Ce3+ ions in the inorganic-organic materials. Ce3+-doped materials were transparent under the preparation conditions of FS/Ce3+ ratio of 100 and 1.0 × 10−4 mol/cm3 of Ce3+ concentration. Optical properties such as absorption spectra, emission spectra and fluorescence quantum yield for the Ce3+-doped hybrid materials were strongly affected by the Ce3+ ion environment. Emission spectra were observed for the Ce3+-doped materials below 400 nm. The emission intensities of the materials prepared from TFTM, CPTM and without FS were approximately 100 times as much as those of materials prepared from APTM and GPTM. The fluorescence quantum yield was highest (11%) for the material prepared from TFTM hybrid materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008643327280
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    Paper (German National Licenses)
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