ZIB

1

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Large-scale expression of recombinant sialyltransferases and comparison of their kinetic properties with native enzymes (1995)

Williams, Mark A. ; Kitagawa, Hiroshi ; Datta, Arun K. ; [et al.]

Springer

Glycoconjugate journal 12 (1995), S. 755-761

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ISSN: 1573-4986

Keywords: sialyltransferase ; glycorprotein ; baculovirus

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract Values ofK m were determined for three purified sialyltransferases and the corresponding recombinant enzymes. The enzymes were Galβ1-4GlcNAc α2-6sialyltransferase and Galβ1-3(4)GlcNAc α2-3sialyltransferase from rat liver; these enzymes are responsible for the attachment of sialic acid to N-linked oligosaccharide chains; and the Galβ1-3GalNAc α2-3sialyltransferase from porcine submaxillary gland that is responsible for the attachment of sialic acid to O-linked glycoproteins and glycolipids. A procedure for the large scale expression of active sialyltransferases from recombinant baculovirus-infected insect cells is described. For the liver enzymes values ofK m were determined using rat and human asialoα1 acid glycoprotein andN-acetyllactosamine as variable substrates; lacto-N-tetraose was also used with the Galβ1-3(4)GlcNAc α2-3sialyltransferase. Antifreeze glycorprotein was used as the macromolecular acceptor for the porcine enzyme. Values forK m were also determined using CMP-NeuAc as the variable substrate.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00731235

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2

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Involvement of Neurospora mitochondrial tyrosyl-tRNA synthetase in RNA splicing. A new method for purifying the protein and characterization of physical and enzymic properties pertinent to splicing (1995)

Saldanha, Roland J. ; Patel, Smita S. ; Surendran, Rajendran ; [et al.]

s.l. : American Chemical Society

Biochemistry 34 (1995), S. 1275-1287

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ISSN: 1520-4995

Source: ACS Legacy Archives

Topics: Biology , Chemistry and Pharmacology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/bi00004a022

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3

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Expression, Purification, and Characterization of Escherichia coli Dihydrodipicolinate Reductase (1995)

Reddy, Sreelatha G. ; Sacchettini, James C. ; Blanchard, John S.

s.l. : American Chemical Society

Biochemistry 34 (1995), S. 3492-3501

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ISSN: 1520-4995

Source: ACS Legacy Archives

Topics: Biology , Chemistry and Pharmacology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/bi00011a002

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4

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Three-Dimensional Structure of Escherichia coli Dihydrodipicolinate Reductase (1995)

Scapin, Giovanna ; Blanchard, John S. ; Sacchettini, James C.

s.l. : American Chemical Society

Biochemistry 34 (1995), S. 3502-3512

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ISSN: 1520-4995

Source: ACS Legacy Archives

Topics: Biology , Chemistry and Pharmacology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/bi00011a003

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5

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X-ray Crystal Structure of the Soybean Agglutinin Cross-Linked with a Biantennary Analog of the Blood Group I Carbohydrate Antigen (1995)

Dessen, Andrea ; Gupta, Dipti ; Sabesan, Subramaniam ; [et al.]

s.l. : American Chemical Society

Biochemistry 34 (1995), S. 4933-4942

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ISSN: 1520-4995

Source: ACS Legacy Archives

Topics: Biology , Chemistry and Pharmacology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/bi00015a004

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6

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Carbon-13 spin relaxation and methyl rotation barriers in the methylethylenes (1975)

Collins, Scott W. ; Alger, Terry D. ; Grant, David M. ; [et al.]

s.l. : American Chemical Society

The @journal of physical chemistry 〈Washington, DC〉 79 (1975), S. 2031-2037

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Source: ACS Legacy Archives

Topics: Chemistry and Pharmacology , Physics

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/j100586a011

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7

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A surface study of cobalt-molybdena-alumina catalysts using x-ray photoelectron spectroscopy (1976)

Patterson, Thomas A. ; Carver, James C. ; Leyden, Donald E. ; [et al.]

s.l. : American Chemical Society

The @journal of physical chemistry 〈Washington, DC〉 80 (1976), S. 1700-1708

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Source: ACS Legacy Archives

Topics: Chemistry and Pharmacology , Physics

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/j100556a011

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8

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Moessbauer spectra of penta- and hexacyanoferrate(II) compounds in solid and frozen solution states (1978)

Monaghan, Charles P. ; Fanning, James C.

s.l. : American Chemical Society

The @journal of physical chemistry 〈Washington, DC〉 82 (1978), S. 1045-1051

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Source: ACS Legacy Archives

Topics: Chemistry and Pharmacology , Physics

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/j100498a018

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9

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Electron transfers involving biological molecules in solution (1978)

Chien, James C. W.

s.l. : American Chemical Society

The @journal of physical chemistry 〈Washington, DC〉 82 (1978), S. 2158-2171

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Source: ACS Legacy Archives

Topics: Chemistry and Pharmacology , Physics

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/j100509a004

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10

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Effect of metal substitution on the molecular structures and functional properties of cytochrome and hemoglobin (1978)

Chien, James C. W.

s.l. : American Chemical Society

The @journal of physical chemistry 〈Washington, DC〉 82 (1978), S. 2171-2179

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Source: ACS Legacy Archives

Topics: Chemistry and Pharmacology , Physics

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1021/j100509a005

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