ISSN:
1432-2242
Keywords:
Somatic hybrid
;
Petunia hybrida
;
Petunia variabilis
;
Protoplast
;
Electrofusion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Somatic hybrid plants were regenerated following electrofusion between leaf mesophyll protoplasts of P. hybrida (2n = 14) and a wild sexually incompatible species, P. variabilis (2n = 18). The selection of hybrids was based on the hybrid vigour, expressed both in the growth of the callus and at the shoot formation stage, resulting from the combination of parental genomes. Calli exhibiting vigorous growth were selected, and upon transfer to regeneration medium gave rise to shoots. Four regenerated plants from three calli had morphological characteristics intermediate between those of the parents. The hybrid nature of these plants was confirmed by chromosome counts as well as isozyme and DNA analyses. They had amphidiploid chromosome numbers (2n = 32) and were fertile. Following self-pollination and backcrossing with P. variabilis, large numbers of F2 and BC1 seedlings were obtained.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00223748
Permalink
