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  • 11
    Electronic Resource
    Electronic Resource
    Segregation for isozymes and HMW glutenins in a doubled-haploid cross of winter wheat carrying 1BL.1RS and 5DL.5RS translocations from rye (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The doubled haploid (DH) wheat line ‘dh 5841’ carrying two translocations from rye, 5DL.5RS and 1BL.1RS, has been crossed to the subline of wheat cultivar ‘Amadeus 7143’ with a 1BL.1RS translocation. The resulting F1 hybrid IJ 98 with a heterozygous 5DL.5DS-5DL.5RS chromosome pair has been used to produce doubled haploids. A total of 57 DH lines were obtained from plantlets regenerated in anther culture after successful colchicine treatment and seed set. These lines were identified regarding the constitution of chromosome 5D (5DL.5DS or 5DL.5RS) by means of isoenzyme marker analysis. Thirty DH lines possessed the 5DL.5DS chromosome, while the remaining 27 lines carried the 5DL.5RS translocation. For some of these lines, the 5DL.5RS chromosome was cytologically confirmed by C-banding. Furthermore, the DH lines were evaluated for their high molecular weight glutenin subunit composition. All possible combinations for the four independent loci —Skdh, Glu-Al, Glu-B1 and Glu-D1— were detected in only 57 DH lines and no segregation distortion was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00638.x
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  • 12
    Electronic Resource
    Electronic Resource
    Application of AFLP for the detection of sex-specific markers in hemp (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two dioecious hemp accessions (Can18 and Can17) were tested by bulked segregant analysis for polymorphisms between male and female bulks with amplified fragment length polymorphisms. Thirty-nine primer combinations were tested and 20 of these yielded one to three male-specific bands. In contrast, no female-specific band was detected. Eight of these primer combinations were used for testing 80 progeny plants from a cross between two plants from Can18 and 30 plants from Can17. A total of 16 and 17 male-specific fragments were obtained for Can 18 and Can 17, respectively. Eleven fragments exhibited the same fragment size in both accessions. All male plants, but not one female plant, showed the respective polymorphic band with each of the eight primer combinations. Problems regarding sex determination under field conditions were successfully overcome by testing plants that had been grown in small pots in a greenhouse. The abundant number of potential markers for the male sex, their complete cosegregation with male plants and the absence of markers for the female sex support the presence of a male sex chromosome in hemp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00620.x
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  • 13
    Electronic Resource
    Electronic Resource
    Evaluation of resistance of apricot cultivars to a Spanish isolate of plum pox potyvirus (PPV) (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The resistance of 12 apricot cultivars to the Dideron type Spanish isolate RB3.30 of plum pox potyvirus was evaluated in controlled conditions in an insect-proof greenhouse. The results of the evaluation demonstrated the resistance of the cultivars ‘Stella’, ‘Stark Early Orange’, ‘Goldrich’, ‘Harcot’, ‘NJA2’, ‘Pandora’ and ‘Avilara’ to the isolate, and the susceptibility of the cultivars ‘A1453’, ‘Bulida’,‘Currot’, ‘Real Fino’ and ‘Canino’
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00457.x
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  • 14
    Electronic Resource
    Electronic Resource
    Marker analysis for quantitative traits in sugar beet (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Many economically important traits are inherited quantitatively and are analysed by breeders in replicated field trials. If dense maps are available, chromosomal regions containing quantitative trait loci (QTL) can be identified and this opens up the possibility of preselecting for quantitative traits in the laboratory. In this study, QTL analysis for yield and yield components in sugar beet is used in two different populations tested in several environments in both populations, QTL were detected for all traits investigated, and their predictive value in breeding schemes was analysed by correlating predicted with observed values. Tolerance to Rhizomania, caused by a gene on chromosome 3, was the main source of genotype-environment interaction in one population, allowing selection on a QTL basis within macro-environments with or without Rhizomania infestation, respectively. No clear results were found for the second population tested in environments with and with-out Cercospora infestation. Consequences for breeding strategies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00482.x
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  • 15
    Electronic Resource
    Electronic Resource
    Selection for sugar yield in sugar beet, Beta vulgaris, using different selection indices (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In sugar beet breeding, sugar yield is mainly influenced by root yield and sugar content. In this short communication several indices to select for both traits simultaneously are compared in order to find the best one. The indices are correlated with the base index of Williams (1962) from independent experiments. The indices differ in the amount of information necessary for the calculation of their weights. Three different series of each eight sites gave similar results. The optimum index using all information from phenotypic and genotypic variances and covariances, did not perform best. Sugar content with its higher herit ability must have a larger weight than root yield. Heritabilities as index weights performed best, but two other indices using heritabilities and phenotypic but no genetic covariances also performed well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00476.x
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  • 16
    Electronic Resource
    Electronic Resource
    Molecular marker analysis of kernel size and shape in bread wheat (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The economic value of wheat grain is determined by the kernel morphology which is an important parameter for manufacturing different food products requiring specific grain characteristics. Although kernel size and shape have emerged as important breeding objectives, not much information is available about the number or location of associated gene(s)/quantitative trait loci. In the present study, a recombinant inbred line population of 106 plants (F7) was phenotyped for four traits, namely kernel length, width, weight and factor form density (FFD) and genotyped with different polymerase chain reaction-based markers. Transgressive segregants were observed for all the traits and genetic correlation studies showed positive correlations between the majority of the traits. The number of markers associated with each trait ranged from two to nine and the phenotypic contribution by an individual marker ranged from 3.3 to 16.6%. Many of the markers showed linkage to more than one trait. Strategies for improving the wheat grain quality traits and the utility of such markers in marker-assisted selection (MAS) efforts are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00896.x
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  • 17
    Electronic Resource
    Electronic Resource
    Susceptibility to sharka (Plum pox virus) in Prunus mandshurica×P. armeniaca seedlings (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Prunus mandshurica [(Maxim.) Koehne] from Central Asia is a species related to apricot and grown in China and Mongolia. This species has been used in apricot breeding as a source of frost resistance. In addition, P. mandshurica has been suggested as the possible origin of some North American apricot cultivars resistant to sharka (Plum pox virus, PPV). The aim of this work was to transmit the resistance to PPV from P. mandshurica to the Spanish apricot cultivar ‘Currot’ by traditional crossing. The resistance to a Dideron PPV isolate of the descendants of P. mandshuricaבCurrot’ and their progenitors was evaluated under controlled conditions in a greenhouse. The results showed the susceptibility of both, the progenitors and the offspring to PPV, as being much higher than in other apricot seedlings. The results showed that the P. mandshurica accession studied is not a good progenitor in breeding apricot for PPV resistance, but just the opposite. The possible role of P. mandshurica as a source of resistance in apricot resistant cultivars is questioned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00902.x
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  • 18
    Electronic Resource
    Electronic Resource
    Molecular cytogenetic characterization of Thinopyrum genomes conferring perennial growth habit in wheat- Thinopyrum amphiploids (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Seven wheat-Thinopyrum amphiploids, AT 3425, AgCs, PI 550710, PI 550711, PI 550712, PI 550713 and PI 550714, were evaluated for perennial growth habit in the field. Three of them, AgCs, AT 3425, and PI 550713, were identified as perennials. Fluorescent genomic in situ hybridization (FGISH) patterns of mitotic chromosomes indicated that AgCs had seven pairs of Thinopyrum chromosomes and 21 pairs of wheat chromosomes. PI 550713 and AT 3425 showed similar FGISH patterns of mitotic chromosomes with three pairs of wheat-Thinopyrum translocated chromosomes, seven pairs of Thinopyrum chromosomes, and 18 pairs of wheat chromosomes. Thinopyrum chromosome pairing in the Fi hybrid of AT 3425 with AgCs demonstrated differences between Thinopyrum genomes in these two amphiploids. Based on chromosome constitutions, pairing and reported pedigrees, AgCs and AT 3425 were identified as a wheat-Thinopyrum elongatum amphiploid and partial wheat-Thinopyrum ponticum amphiploid, respectively. Chromosome pairing in the F1 hybrid between AT 3425 and PI 550713 revealed that these two amphiploids contained the same Thinopyrum genome. Two different Thinopyrum genomes conferring perennial growth habit were identified from the perennial amphiploids and characterized cytogenetically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00560.x
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  • 19
    Electronic Resource
    Electronic Resource
    Screening Cucumis sativus landraces for resistance to cucumber vein yellowing virus (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Cucumber production in Spain is being threatened by the cucumber vein yellowing virus (CVYV), which has been causing severe yield losses since it was first detected in 2000. Although most of the Spanish cucumber production is based on hybrid cultivars, farmers have grown cucumber landraces for centuries. A collection of 46 Spanish cucumber landraces, four melon cultivars and one accession of Cucumis africanus, were evaluated for resistance to CVYV. An isolate of CVYV from Almeria, closely related to CVYV from Israel, was used. CVYV was mechanically transmitted and detected by reverse transcriptase polymerase chain reaction and molecular hybridization. Only mild infections were observed in melons, while the wild species C. africanus was susceptible to this disease. Most of the cucumber accessions assayed were highly susceptible to CVYV and showed vein-clearing symptoms, severe growth reduction and a high viral accumulation. Several cucumber accessions were partially resistant to this virus, displaying mild symptoms and a significantly reduced viral accumulation. These partially resistant varieties could be cultivated under integrated production systems, and also be used as resistance sources to develop new cucumber hybrids resistant to CVYV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00882.x
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  • 20
    Electronic Resource
    Electronic Resource
    Detection of endophytic fungi in Festuca spp. by means of tissue print immunoassay (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Many wild and cultivated grasses live in mutualistic symbiosis with endophytic fungi of the genera Neotyphodium and Epichloë. These associations are of agronomic importance because endophytes may induce a range of beneficial effects for the host plant but also produce alkaloids detrimental to livestock. Conventional detection of endophytes by means of histological staining is time-consuming and not suited for large numbers of samples. Therefore, in order to simplify the detection of endophytic fungi the utility of tissue print immunoassay (TPIA) was studied and compared with the commonly used microscopic analysis. Ecotypes collected from natural grassland habitats and plants from field experiments were analyzed for endophyte infection. Both methods provided similar results. Based on stained or non-stained mycelium in tissue prints, endophyte-infected (E +) and endophyte-free (E-) tillers and inflorescences of Festuca pratensis, F. arundinacea and F. rubra were clearly distinguishable. Prints of cross sections of tillers allow the precise localization of endophyte infection within the plant tissues. Because TPIA allows the examination of endophytes in individual branches and segments of inflorescences it is a useful method for dissemination studies. Tissue print immunoassay appears to be a reliable method suited for routine work in research, practical grassland management and selection of defined E + or E- material for breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00855.x
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