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  • Electronic Resource  (6)
  • 1995-1999  (5)
  • 1970-1974  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Triticum aestivum cv. ‘Norin 26’ with Aegilops crassa cytoplasm shows photoperiod-sensitive cytoplasmic male sterility (PCMS). This alloplasmic line is almost completely male-sterile under long-day conditions (≥ 15h), but highly male-fertile under short-day conditions (≤ 14.5h). To obtain male—fertile mutants against PCMS, seeds of the alloplasmic line were treated with ethyl methane sulfonate (EMS). The M3 generation was evaluated for PCMS expression, and one fertility-restoring (FR-mutant) line showing high male fertility under the long-day conditions was selected. Reciprocal F, hybrids between the FR-mutant and the alloplasmic ‘Norin 26’ showed male sterility under the long-day conditions, and continuous segregation with respect to the degree of fertility restoration occurred in their F2 generations. These results indicate that multiple recessive mutations with minor effects, induced in the nuclear genome, are involved in the fertility restoration. In fact, no restriction-fragment-length polymorphisms of mitochondrial DNA between the FR-mutant and the alloplasmic ‘Norin 26’ are found.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 30 (1996), S. 509-514 
    ISSN: 1432-0983
    Keywords: Key words Mitochondria ; cox genes ; Transcription ; Alloplasmic wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Wheat plants with Aegilops columnaris cytoplasm are characterized by growth inhibition and partial male sterility and show an impaired mitochondrial cytochrome c oxidase activity. We designed this study to clarify the functional relationship between this impaired cytochrome c oxidase activity and possible structural alterations to the mitochondrial genes cox1, cox2 and cox3 that encode three subunits of the cytochrome c oxidase. Using restriction mapping and DNA sequencing, we found major rearrangements in the flanking regions of the cox1 and cox3 genes. In Northern-blot analysis, we detected two different cox3 transcripts and a reduced level of a cox2 transcript, whereas we could not detect a transcript of cox1. These results suggest that rearrangements of the flanking regions of the cox1 gene may result in the severe suppression of its transcription, and subsequently, may lead to the impaired cytochrome c oxidase activity.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 45 (1974), S. 104-109 
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In addition to the already known Aegilops caudata cytoplasm, the cytoplasms of five Aegilops species, all belonging to the section Polyeides, were found to induce haploids (11–56%) and twins (0.5–15%) in a common wheat, Salmon, at high frequencies. The great majority of the twin pairs were of the diplo-haplo type. The origin of both the haploids and twins was ascribed to the induction of parthenogenesis in Salmon by the alien cytoplasm. Pollen parents produced some differences in haploid frequency. The distribution of the parthenogenesis-inducing cytoplasms in the genus Aegilops is discussed in relation to the phylogeny of the donor species.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2242
    Keywords: Key words Intraspecific variation ; Interspecific variation ; Aegilops Sitopsis species ; RFLP analysis ; Nuclear DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The level of intra- and interspecific variations on nuclear DNA in five Aegilops species of the Sitopsis section were investigated using restriction fragment length polymorphism (RFLP) analysis. A total of 18 accessions, i.e. 7 of Ae. speltoides, 3 of Ae. longissima, 2 of Ae. searsii, 3 of Ae. sharonensis and 3 of Ae. bicornis, were used. One accession each of Triticum aestivum, T. durum, T. urartu and Ae. squarrosa was included as reference material. Five enzymes and 20 probes were used. Among the five Sitopsis species studied, Ae. speltoides had the largest intraspecific variation which was as high as the interspecific variation observed among the other four species. The section Sitopsis was divided into two distinct groups: one containing only Ae. speltoides and the other, Ae. longissima, Ae. searsii, Ae. sharonensis and Ae. bicornis. This grouping by RFLP analysis is in agreement with the taxonomical classification of the subsections.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2242
    Keywords: Intraspecific variation ; Interspecific ; variation ; Aegilops Sitopsis species ; RFLPanalysis ; Nuclear DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The level of intra- and interspecific variations on nuclear DNA in five Aegilops species of the Sitopsis section were investigated using restriction fragment length polymorphism (RFLP) analysis. A total of 18 accessions, i.e. 7 of Ae. speltoides, 3 of Ae. longissima, 2 of Ae. searsii, 3 of Ae. sharonensis and 3 of Ae. bicornis, were used. One accession each of Triticum aestivum, T. durum, T. urartu and Ae. squarrosa was included as reference material. Five enzymes and 20 probes were used. Among the five Sitopsis species studied, Ae. speltoides had the largest intraspecific variation (π=0.061), which was as high as the interspecific variation observed among the other four species. The section Sitopsis was divided into two distinct groups: one containing only Ae. speltoides and the other, Ae. longissima, Ae. searsii, Ae. sharonensis and Ae. bicornis. This grouping by RFLP analysis is in agreement with the taxonomical classification of the subsections.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 90 (1995), S. 129-134 
    ISSN: 1432-2242
    Keywords: Wheat ; RFLP ; Triticum dicoccoides ; T. araraticum ; Genetic divergence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Intra- and inter-specific variations in the nuclear DNA of Triticum dicoccoides Körn. (2n = 28, genome constitution AABB) and T. araraticum Jakubz. (2n = 28, AAGG), wild species, respectively, of the Emmer and Timopheevi group, were studied by restriction fragment length polymorphism (RFLP) analysis. Total DNAs of 32 T. dicoccoides and 24 T. araraticum accessions, collected from throughout the distribution areas of these species, were treated with two 6-bp cutters and hybridized with 30 nuclear DNA clones as probes to detect RFLPs. A total of 167 hybrid bands were observed per accession. All the enzyme-probe combinations showed RFLPs between accessions. The average genetic distance between the T. dicoccoides accessions was 0.0135 ± 0.0031 and that between the T. araraticum accessions 0.0036 ± 0.0015, indicative of about a four-fold intraspecific variation in T. dicoccoides as compared to T. araraticum in terms of genetic distance. No significant genetic differentiation was found for the geographical populations of these species, the genetic distance between the two species being 0.0482 ± 0.0022. The interspecific divergence corrected for intraspecific divergence was 0.0395, about three times that for T. dicoccoides and 11 times that for T. araraticum. The results show that in the wild state the Emmer and Timopheevi groups are clearly differentiated and that T. dicoccoides has much greater variation than T. araraticum, suggesting a relatively recent origin for the latter and therefore a diphyletic origin for these species.
    Type of Medium: Electronic Resource
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