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  • Electronic Resource  (4)
  • 1995-1999  (4)
  • Map-based cloning  (2)
  • aluminosilicate gel  (2)
Material
  • Electronic Resource  (4)
Years
  • 1995-1999  (4)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 251 (1996), S. 365-372 
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; Dominant markers ; Map-based cloning ; Recombinant selection ; T-DNA localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The map positions of a set of eight T-DNA insertions in theArabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar),β-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). Theneo, hpt andbar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing thehpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of sol gel science and technology 9 (1997), S. 169-181 
    ISSN: 1573-4846
    Keywords: aluminosilicate gel ; non-linear absorption ; metalloporphyrin ; spirooxazine ; time-resolved spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Linear and nonlinear optical effects have been studied in chromophore-doped gel hosts. Tetra-4-sulfonatophenylporphyrinatocopper(II) (CuTPPS),and1, 3-dihydro-1,3,3-trimethylspiro[2H-indole-2, 3′-[3H]-naphth[2,1-b][1,4]oxazine] (SP spirooxazine) species were entrapped within porous aluminosilicate hosts.Optical limiting effects and radiative up-conversion behavior in the CuTPPS-doped materials are described, and a six-level model is proposed based on experimental findings. Spirooxazine-containing specimens exhibiting photochromic effects were prepared, and cw and time-resolved spectroscopy methods are used to assess excited state band structures and the nature of guest-host interactions in the resultant gels.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of sol gel science and technology 9 (1997), S. 169-181 
    ISSN: 1573-4846
    Keywords: aluminosilicate gel ; non-linear absorption ; metalloporphyrin ; spirooxazine ; time-resolved spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Linear and nonlinear optical effects have been studied in chromophore-doped gel hosts. Tetra-4-sulfonatophenylporphyrinatocopper(II) (CuTPPS), and 1,3-dihydro-1,3,3-trimethylspiro[2H-indole-2,3′-[3H]-naphth[2,1-b][1,4]oxazine] (SP spirooxazine) species were entrapped within porous aluminosilicate hosts. Optical limiting effects and radiative up-conversion behavior in the CuTPPS-doped materials are described, and a six-level model is proposed based on experimental findings. Spirooxazine-containing specimens exhibiting photochromic effects were prepared, and cw and time-resolved spectroscopy methods are used to assess excited state band structures and the nature of guest-host interactions in the resultant gels.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1617-4623
    Keywords: Key words Arabidopsis thaliana ; Dominant markers ; Map-based cloning ; Recombinant selection ; T-DNA localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar), β-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
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