ZIB

Digitale Medien

A comparison of the antigenicity of the extracellular products and whole-cell sonicates from Mycobacterium spp. in rabbits, mice and fish by immunoblotting and enzyme-linked immunosorbent assay (1997)

Chen, S.-C. ; Adams, A. ; Thompson, K.D. ; [weitere]

Oxford, UK : Blackwell Science Ltd

Journal of fish diseases 20 (1997), S. 0

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ISSN: 1365-2761

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie , Medizin

Notizen: The antigenicity of extracellular products (ECPs) derived from Mycobacterium spp. isolated from snakehead, Channa striata (Bloch), and Siamese fighting fish, Betta splendens (Regan), were examined by immunoblotting and enzyme-linked immunosorbent assay (ELISA) using sera collected from immunized rabbits, mice and fish (rainbow trout). All three species responded to a 65-kDa protein present in both the ECPs and whole cell sonicates (WCSs) from a variety of Mycobacterium spp. Cross-reactivity of anti-M. tuberculosis and anti-human heat-shock protein monoclonal antibodies (MAbs), and the presence of fibronectin binding proteins secreted into ECPs of mycobacteria were also examined. The MAbs against human 60-kDa heat-shock protein cross- reacted with the band at 65 kDa in the ECPs of TB1 (isolated from snakehead fish) and the type strain M. marinum, while the anti-M. tuberculosis MAb F29–47 elicited a strong reaction with a band at 21 kDa with most of the ECPs from mycobacterial strains examined. The major fibronectin-binding proteins were located between 21 and 25 kDa. The 65-kDa protein from ECPs of Mycobacterium spp. proved strongly immunogenic to rabbits, mice and fish. Rabbit antiserum against the 65-kDa protein from strain TB267 reacted with many non-Mycobacterium WCSs, and therefore, the 65-kDa protein from Mycobacterium spp. is believed to be a common protein found in many fish bacterial pathogens.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1046/j.1365-2761.1997.00322.x

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Digitale Medien

Structure of the DNA Octanucleotide d(ACGTACGT)2 (1996)

Wilcock, D. J. ; Adams, A. ; Cardin, C. J. ; [weitere]

Copenhagen : International Union of Crystallography (IUCr)

Acta crystallographica 52 (1996), S. 481-485

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ISSN: 1399-0047

Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001

Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik

Notizen: d(ACGTACGT), C78H84N30O32P7.20H2O, Mr (DNA) = 2170, tetragonal, P43212 (No 96), a = 42.845 (1), b = 42.845(1), c = 24.804 (1) Å, V = 45532.5 (2) Å3, z = 8,λ(MoKα) = 0.71069 Å,µ(MoKα) = 0.10 mm−1, T = 295 K, R = 0.18 for 1994 unique reflections between 5.0 and 1.9 Å resolution. The self-complementary octanucleotide d(ACGTACGT)2 has been crystallized and its structure determined to a resolution of 1.9 Å. The asymmetric unit consists of a single strand of octamer with 20 water molecules. It is only the second example of an octanucleotide having terminal A·T base pairs whose structure has been determined by X-ray crystallography. The sequence adopts the modified A-type conformation found for all octanucleotide duplexes studied to date with the helix bent by approximately 15° and an average tilt angle of 0°. Unusually the data collection was carried out using a 3 kW molybdenum sealed-tube source. The conformational details are discussed in comparison with other closely related sequences.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1107/S0907444995016052

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Digitale Medien

Cyanobacteria carrying ansmt-lux transcriptional fusion as biosensors for the detection of heavy metal cations (1996)

Erbe, J L ; Adams, A C ; Taylor, K B ; [weitere]

Springer

Journal of industrial microbiology and biotechnology 17 (1996), S. 80-83

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ISSN: 1476-5535

Schlagwort(e): cyanobacteria ; biosensor ; heavy metal ; lux

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract The metal-responsivesmt operator/promoter region ofSynechococcus PCC7942 was fused to theluxCDABE genes ofVibrio fischeri. Plasmid DNA (pJLE23) carrying this fusion conferred metal ion-inducible luminescence to transformed cyanobacteria.Synechococcus PCC7942 (pJLE23) was sensitive to ZnCl2 concentrations within a range of 0.5–4 μM as demonstrated by induction of luminescence. Trace levels of CuSO4, and CdCl2 were also detected.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01570047

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Digitale Medien

Search Costs and Price Dispersion in a Localized, Homogeneous Product Market: Some Empirical Evidence (1997)

Adams, A. Frank

Springer

Review of industrial organization 12 (1997), S. 801-808

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ISSN: 1573-7160

Schlagwort(e): Information costs ; price dispersion ; search costs

Quelle: Springer Online Journal Archives 1860-2000

Thema: Wirtschaftswissenschaften

Notizen: Abstract Convenience stores provide an opportunity to compare the price dispersion that occurs for gasoline, which is characterized by relatively low search and information costs to consumers, with that of in-store items, which are characterized by relatively high search and information costs to consumers. The results obtained from this study support the hypothesis that differences in search and information costs for consumers play a significant role in explaining the observed price dispersion that occurs for homogeneous items sold at convenience stores.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1007779422525

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Digitale Medien

Ultrastructural changes in cultured human periodontal ligament cells exposed to dental materials (1995)

Adams, A. M. ; Soames, J. V. ; Searle, R. F.

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 29 (1995), S. 999-1004

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ISSN: 0021-9304

Schlagwort(e): Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin , Technik allgemein

Notizen: The cytopathic effects of a range of dental restorative cements were examined by electron microscopy using an in vitro model with cultured test cells derived from human periodontal ligament. Monolayers were fixed and processed in situ after 2, 24, and 48 h exposure. Many cells showed lysis after two hours exposure consistent with immediate acute injury. Cultures subsequently recovered, reflecting different susceptibilities of cells to injury, and at later stages showed distinct patterns of cell damage in response to different restorative materials. These were related primarily to either cytoplasmic or nuclear damage and to changes resembling apoptosis. © 1995 John Wiley & Sons, Inc.

Zusätzliches Material: 10 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jbm.820290812

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