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  • Electronic Resource  (7)
  • 1990-1994  (2)
  • 1980-1984  (5)
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  • Electronic Resource  (7)
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  • 1
    Electronic Resource
    Electronic Resource
    Vitamin D-Dependent Calcium-Binding Protein Is Highly Conserved in the Metanephros (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 435 (1984), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1984.tb13812.x
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  • 2
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 208 (1984), S. 233-242 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Insulin, glucagon, somatostain, and pancreatic polypeptide (PP) were localized in the pancreas of the common garter snake, Thamnophis sirtalis, by light and transmission electeron microscopic (TEM) immunocytochemistry. Colloidal gold-protein A was used for TEM localization and the peroxidase containing A cells and the insulin-positive B cells were the most numerous cell types. The somatostatin-containing D cells made up about 15% of the endocrine cells. PP-positive F cells were a minor cell type. The only topographic arrangement of the cells within the endocrine-rich areas that was apparent was the peripheral localization of the D and F cells. Cells of a specific cell type were sometimes grouped together. At the elctron microscopic (EM) level, the gold particles (indicating the pesence of hormone) were localized nearly exclusively over the secretory grnules of the ractive cells. The α-granules were the largest found and were predominantly electron dense with a moderately electron-dense periphery. PP-containing granules were the smallest. The somatostatin-reactive δ-granules were round and moderately electron opaque. The β-granules were heterogeneous in appearance. The morphognomy of the secretory granules of the major endocrine cell types is qualitatively similar to that of mammals. Wheter or not the quantitative and/or associative differences contribute to the marked metabolic differences between reptiles and mammals, remains to be determined.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092080210
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  • 3
    Electronic Resource
    Electronic Resource
    Cellular gene expression for calbidin-D28k in mouse kidney (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 227 (1990), S. 145-151 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Gene expression for calbindin-D28k, the 28,000 relative molecular mass vitamin D-dependent calcium-binding protein, was measured in cells of the murine nephron by in situ hybridization on tissue sections (hybridization cytochemistry). Radiolabeled (35S-UTP), single-stranded RNA complementary to calbinding-D28k-mRNA (probe RNA) was prepared from linearized cDNA template and used for the hybridizations. Autoradiography was carried out and cellular levels of hybridization signal (silver grains) were quantified. After corretion for background the concentration of silver grains was more than 350% greater in the distal tubule than in either the proximal tubule or the glomerulus. The relative cellular level of mRNA in the cytoplasm, as reflected in silver grains/cell, of the distal tubules with probe RNA was 3.4 times greater than that with control RNA. Cells of the distal tubule were the only apparent sites of specific hybridization with probe RNA. The presence of calbindin-D28k-mRNA in the distal tubule corresponded to the localizations of calbindin-D28k by immunocytochemistry.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092270202
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  • 4
    Electronic Resource
    Electronic Resource
    Occurrence and localization of calbindin-D28k in kidney and cerebellum of the slider turtle, Trachemys scripta (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 239 (1994), S. 185-190 
    Details
    ISSN: 0003-276X
    Keywords: Calbindin-D28k ; CaBP ; Turtle ; Immunocytochemistry ; Kidney ; Brain/cerebellum ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Backgrouns: Since its initial discovery in the avian intestine, calbindin-D28k has been reported to occur in various species and tissues. Although calbindin-D28k binds calcium ions in the physiologically relevant range of intracellular calcium, its functional role in the various cell types where it has been localized remains unknown.Methods: We examined the occurrence of calbindin-D28k in the brain and kidney of the testudine reptile, Trachemys scripta, by immunoblotting and immunocytochemistry using rabbit anti-sera directed against rat renal calbindin-D28k and chicken intestinal calbindin-D28k.Results: Immunoblotting revealed the presence of calbindin-D28k in the turtle tissues. A single immunoreactive band in the 28,000 relative molecular mass region was visualized in cerebellar and renal homogenates. Immunocytochemistry revealed reaction product for the presence of calbindin-D28k in the Purkinje cells of the cerebellum, and in the distal tubular cells of the nephron. Processes as well as the perikaryon of the Purkinje cell were immunoreactive.Conclusion: This study describes the occurrence and cellular localization of calbindin-D28k in a reptilian cerebellum, and confirms the phylogenetic distribution of renal calbindin-D28k to the oldest major reptilian group. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092390209
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  • 5
    Electronic Resource
    Electronic Resource
    Four hormones in the pancreas of the lizard, Anolis carolinensis (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 199 (1981), S. 89-97 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The electron microscopic localization of insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) in the pancreas of the iguanid lizard, Anolis carolinensis was studied by the unlabeled antibody peroxidase-antiperoxidase immunocytochemical technique. Insulin, glucagon, and somatostatin were localized absolutely to those cells previously identified on the basis of the characteristics of their secretory granules as being β cells, α cell, and D cells, respectively. The secretory granule cores of the PP-containing cells appeared to be ellipsoidal with a semi-major axis of 450 nm and a semi-minor axis of 365 nm. This previously unidentified cell type is named the F cell, in keeping with the localization of PP to the original F cell of the canine pancreas. Without immunocytochemical staining, the qualitative ultrastructural characteristics of the F cell secretory granules were inadequate to permit certain identification of the F cell, especially with regard to the D cell.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091990109
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  • 6
    Electronic Resource
    Electronic Resource
    Continuous-Perifusion tissue culture of fetal and adult pancreas of the lizard Anolis carolinensis (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 203 (1982), S. 165-173 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The differentiation of the fetal saurian pancreas in continuousperifusion tissue culture (CPTC) was examined. Splenic pancreases from 24-day postoviposition fetuses of the green anole, Anolis carolinensis, were grown for 8 to 31 days by CPTC following successful preliminary studies with adult pancreas. Adult anolian endocrine pancreas was maintained for up to 7 days by CPTC. The pancreatic explants were examined morphologically by light and electron micros-copy. The functional integrity of the endocrine cells was evaluated by measuring hormone levels of the explants and in the basal medium and by determining the kinetics of hormone release. The pancreatic endocrine cells from fetal and adult anoles were functionally and morphologically intact after CPTC. The exocrine pancreas was not maintained during culture. This study demonstrates for the first time the growth of the reptilian endocrine pancreas in culture.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092030115
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  • 7
    Electronic Resource
    Electronic Resource
    An immunocytochemical study of the cytogenesis of pancreatic endocrine cells in the lizard, Anolis carolinensisDedicated in memory to the late Irwin L. Baird, Ph.D. (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 163 (1982), S. 181-193 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The differentiation of the pancreatic endocrine cells in the lizard Anolis carolinensis following oviposition was examined. Immediately postoviposition (PO) there was no apparent differentiation of epithelioid cells into endocrine or exocrine components. Individual subpopulations of the endocrine-like cells, which could not be identified during the early PO period on the basis of either their tinctorial properties at the light-microscopic level or their granule morphologies at the electron-microscopic level, exhibited specific hormonal localization by peroxidase-antiperoxidase complex immunocytochemistry. All four hormones searched for, insulin, glucagon, somatostatin, and pancreatic polypeptide (PP), were present in epithelioid cells shortly after oviposition. However, the immunostained secretory granules in the early PO period were smaller than those of the adult. Secretory granule morphologies that are typical of the adult were acquired at different times during development. Delta granules were observed first and were followed by alpha granules, and beta granules which appeared shortly before birth. The secretory granules of the PP-containing F cells could not be readily placed within this maturation sequence. Mosaic cells (containing more than one hormone) were not seen. Levels of immunoreactive insulin and glucagon in the pancreas increased several fold from day 10 to day 28 PO, but the attainment of adult beta-granule morphologies did not appear to be directly related to insulin itself. The results show that cytodifferentiation of the anolian endocrine pancreas occurs postoviposition and that immunocytochemical methods can be used to follow an organelle sequence during development. These findings suggest that subcellular organelles undergo structural remodeling during maturation which, at least in the case of secretory granules, may have functional significance.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001630207
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