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  • Electronic Resource  (3)
  • 1990-1994  (3)
  • Ia antigen  (2)
  • Guinea pig skin  (1)
  • (Achromobacter lyticus)
  • Axoneme
Material
  • Electronic Resource  (3)
Years
  • 1990-1994  (3)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 282 (1990), S. 253-257 
    ISSN: 1432-069X
    Keywords: Langerhans cells ; Ia antigen ; Lectins ; Subpopulation ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Lectin-binding profiles of epidermal Langerhans cells (LCs) were investigated in three strains of mice using immunofluorescence procedures. Three lectin-binding profiles were observed in each strain of mice. Most epidermal LCs reacted with concanavalin A (Con A) and Ricinus communis agglutinin 1 (RCA-1), whereas none reacted with Dolichos biflorus agglutinin (DBA). Peanut agglutinin (PNA) and wheat germ agglutinin (WGA) reacted with some of the epidermal LCs. These binding profiles were similar from site to site of the body in all strains of mice. We also investigated the lectin-binding profiles of epidermal Ia positive (Ia+) cells migrating into the grafted skin up to 165 days after transplantation. BALB/c (H-2d) murine skin was grafted onto the back of (C3H/He×BALB/c)F1 (H-2k×H-2d) mice. The percentages of migrating I-A+ epidermal cells reactive with PNA and WGA were different from those of the normal epidermis soon after grafting and reached a normal level at 43 days after grafting. Our results demonstrated that there is a heterogeneous population of epidermal LCs defined by lectin-binding profiles.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 282 (1990), S. 115-119 
    ISSN: 1432-069X
    Keywords: Histamine H1-receptor ; [3H]Mepyramine binding ; Vascular endothelial cell ; Guinea pig skin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In this study, a new procedure for the cultivation of dermal vascular endothelial cells from guinea pig ear lobe was established, and specific binding of [3H]mepyramine to the cultured cell membranes was demonstrated. The vascular endothelial cells were successfully isolated from the dermis of guinea pig ear lobes by the intravenous injection of dispase. Guinea pig serum, as a supplementary component in culture medium, is more suitable for the culture of guinea pig dermal vascular endothelial cell than is fetal calf serum. The [3H]mepyramine binding study indicated the presence of a large number of H1-receptors on the membrane of dermal vascular endothelial cells of guinea pigs, suggesting the important role of endothelial cells in cutaneous reactions to histamine.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 282 (1990), S. 415-417 
    ISSN: 1432-069X
    Keywords: Interferon-γ ; Tumour necrosis factor-α ; Ia antigen ; Keratinocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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