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  • Electronic Resource  (6)
  • 1990-1994  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Phylogenetic relationships of annual and perennial wild rice: probing by direct DNA sequencing (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 693-702 
    Details
    ISSN: 1432-2242
    Keywords: Asian wild rice ; Phytochrome intron ; Polymerase chain reaction (PCR) ; Direct DNA sequencing ; Intraspecific DNA polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The phylogenetic relationships between Asian wild rice strains were analyzed by direct sequencing of PCR-amplified DNA fragments. The sequence of three introns located in the phytochrome gene was determined for eight strains of the Asian wild rice, Oryza rufipogon, and one strain of the related African species, Oryza longistaminata. The number of nucleotide substitutions per site between various strains within a single species, O. rufipogon, ranged between 0.0017 and 0.0050, while those between two related species, O. rufipogon and O. longistaminate, were 0.043–0.049 (23–26 within 532 bp). Taken together with the sequence differences of the 10-kDa prolamin gene, a model is proposed for the phylogenetic relationships and evolutionary history of annuals and perennials within O. rufipogon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226739
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Replication in vitro of the influenza virus genome: selective dissociation of RNA replicase from virus-infected cell ribonucleoprotein complexes (1994)
    Springer
    Archives of virology 136 (1994), S. 269-286 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Replication of the influenza virus genome involves two discrete step reactions: vRNA-directed primer-independent (unprimed) synthesis of cRNA; and cRNA-directed unprimed synthesis of vRNA. Nuclear extracts from both MDCK and HeLa cells infected with influenza virus A/PR8/34 exhibited unprimed synthesis of both cRNA and vRNA strands (a parameter of RNA replication). Ribonucleoprotein (RNP) complexes with the replication activity were isolated from these nuclear extracts by glycerol gradient centrifugation in the presence of 0.1 M KCl. At 0.5 M KCl, however, these complexes were dissociated into stripped RNP and soluble protein fractions. The soluble fraction contained the activity of exogenous template-dependent unprimed RNA synthesis, indicating that the RNA replicase is dissociated from RNP upon exposure to high salt concentrations. On the other hand, the high salt-treated RNP catalyzed only primer-dependent RNA synthesis, but regained a low level activity of exogenous template-dependent unprimed RNA synthesis by adding nuclear extracts from uninfected cells, suggesting that host factor(s) is involved in the functional interconversion of influenza virus RNA polymerase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01321057
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of a temperature-sensitive mutant in the RNA polymerase PB2 subunit gene of influenza A/WSN/33 virus (1990)
    Springer
    Archives of virology 114 (1990), S. 65-73 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The temperature-sensitive mutant ts-1 of influenza virus A/WSN/33 carries mutations in the gene encoding RNA polymerase PB2 subunit. Effect of temperature on various steps of viral RNA synthesis was examined using disrupted virions of ts-1 mutant. The initiation of RNA synthesis with dinucleotide ApG primer was not affected by elevated temperature, whereas that with primer RNA containing 5′-terminal cap-1 structure was temperature-sensitive. The result supports the previous notion deduced from the UV-crosslinking experiments, that PB2 is involved in the cap-1 dependent initiation of RNA synthesis. In addition, the ts-1 mutant showed a defect in RNA chain elongation. Nucleotide sequence analysis of RNA segment 1 of ts-1 mutant revealed that the amino acid number 417 is essential for the recognition of cap-1 structures and/or the interaction with catalytic unit of the RNA polymerase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311012
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Temporal control for translation of influenza virus mRNAs (1991)
    Springer
    Archives of virology 120 (1991), S. 33-42 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary cDNAs for genome RNAs of influenza virus A/PR/8/34 were cloned and portions containing the ATG initiation codon for translation were inserted into the 5′ leader sequence of the chloramphenicol acetyltransferase (CAT) gene in a pSV2cat vector. In cells that were transfected with a plasmid containing a cDNA segment for the early gene and then super-infected with influenza virus, the maximal CAT activity was obtained at the early stage of infection. In contrast, a plasmid containing a cDNA segment for the late gene directed the highest activity at the late stage of infection. These observations together with the previous observations [K. Yamanaka et al. (1988) Virus Genes 2: 19–30] indicate that the translational efficiency of influenza viral mRNA is subjected to temporal control following viral infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310947
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Regulatory effects of matrix protein variations on influenza virus growth (1993)
    Springer
    Archives of virology 133 (1993), S. 283-294 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Influenza virus A/WSN/33 forms large plaques (〉 3 mm diameter) on MDCK cells whereas A/Aichi/2/68 forms only small plaques (〈 1 mm diameter). Fast growing reassortants (AWM), isolated by mixed infection of MDCK cells with these two virus strains in the presence of anti-WSN antibodies, all carried the M gene from WSN. On MDCK cells, these reassortants produced progeny viruses as rapidly as did WSN, and the virus yield was as high as Aichi. The fast-growing reassortants overcame the growth inhibitory effect of lignins. Pulse-labeling experiments at various times after virus infection showed that the reassortant AWM started to synthesize viral proteins earlier than Aichi. Taken together, we conclude that upon infecting MDCK cells, the reassortant viruses advance rapidly into the growth cycle, thereby leading to an elevated level of progeny viruses in the early period of infection. Possible mechanisms of the M gene involvement in the determination of virus growth rate are discussed, in connection with multiple functions of the M proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01313769
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular anatomy of viral RNA-directed RNA polymerases (1994)
    Springer
    Archives of virology 134 (1994), S. 235-258 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310564
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    Paper (German National Licenses)
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