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  • Electronic Resource  (3)
  • 1990-1994  (3)
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  • Electronic Resource  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Mite fauna, Der p I, Der f I and Blomia tropicalis allergen levels in a tropical environment (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fifty dust samples were collected from the mattresses and bedroom floors of 25 subjects with allergic asthma in Cartagena, Colombia, in order to identify house dust mites and quantitate Der p I, Derf I and Blomia tropicalis allergens. The geometric mean of the total mite density per gram of dust was 418 (range, 40–2280). Twenty-two samples (44%) had more than 500 mites and four, less than 100. B. tropicalis and Dermatopha-goides pteronyssinus were found in 96% and 90% of the samples, accounting for 40.1% and 35.7% of the total mites, respectively. Cheyletus malaccensis, Chortoglyphus arcuatus, Pyroglyphus africanus, Orihatids, Grallacheles bakeri. Tarsonemus spp., Suidasia spp., Dermatophagoides farinae and unidentified mites accounted for the rest. The geometric mean of the total mites/gram of dust in mattresses (563.9) was significantly higher than in floor dust (309. 1), P 〈 0.01. Allergen concentrations and mite numbers were analysed by Spearman rank correlations: B. tropicalis mites vs B. tropicalis allergen, r= 0.54, P〈0.001; D. pteronyssinus mites vs Der p I, r= 0.52, P〈 0.001. A negative correlation was obtained between B. tropicalis mites and Der p I. Allergens derived from B. tropicalis and other domestic mite species may play an important role in sensitization and allergic symptoms in Cartagena, Colombia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1993.tb00325.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sensitization to Chortoglyphus arcuatus and Aleuroglyphus ovatus in Dermatophagoides spp. allergic individuals (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The prevalence of specific IgE to the storage mites Aleuroglyphus ovatus (Ao) and Chortoglyphus arcuatus (Ca) was studied in 77 individuals with allergic asthma and/or chronic allergic rhinitis. All these individuals had a positive skin test (weal ≥ 3 mm) to extracts of Dermatophagoides pteronyssimis (Dp) and/or Dermatophagoides farinac (Df). Sera from 29 non-atopic individuals were used as controls. A RAST was considered positive when a serum bound ≥1% of the total counts added. The prevalence of a positive RAST to Dp was 75.3%, and to at least one of the two storage mites (Ao and Ca), 76.6%. Among patients with a positive RAST to Dp. 79.3% and 75.8% were RAST positive to Ao and Ca, respectively. RAST inhibition studies with a pool of sera from 13 subjects with high RAST binding to all three mites showed significant crossreactivity between Ao and Ca and minimal to moderate crossreactivity between Dp and Ao and Co, This study demonstrates that Sensitization to Ao and Ca is common in individuals with respiratory allergies in Cartagena, Colombia and suggests that Ao, Ca and Dp have unique and common allergenic determinants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1993.tb00306.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Allergenicity of the cat flea (Ctenocephalides felis felis) (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Adult fleas, spent and unspent culture media were extracted and the radio-atlergosor-bent test (RAST) performed with sera of 48 cat flea skin test-positive individuals from the Tampa Bay area of Florida. Sixteen sera (33.6%) had a positive RAST to the cat flea extract prepared in our laboratory [1.7-11.4% of the total counts (TC) added]. Six of the 16 sera (12.5%) also contained specific IgE to allergens in thespent medium (0.8-3.3% TC). The allergen composition and strength were studied by RAST inhibition of two commercial cat flea extracts and compared with our in-house flea extract. The results demonstrated similar allergen compositions and different potencies. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the in-house flea extract showed several Coomassie blue-stained bands (10–85 kD). SDS-PAGE immunoblols revealed five IgE-binding bands at 34, 35, 39, 54 and 60 kD. Flea allergens were quantified in eight house dust samples using RAST inhibition assays and expressed as RAST inhibition units; five of these samples contained detectable levels. Cat flea allergens may contribute to the allergenicity of house dust in areas of heavy llea infestation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1993.tb00342.x
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    Paper (German National Licenses)
    Fulltext
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