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  • 1
    Electronic Resource
    Electronic Resource
    Identification of allergens from the mite Blomia tropicalis (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 24 (1994), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In some tropical areas the mite Blomia tropicalis is a clinically important allergenic component of house dust, inducing specific IgE immune response in patients with allergic respiratory diseases such as asthma and rhinitis. The identification of allergens of this mite is necessary to obtain appropriate reagents for diagnostic and treatment procedures. We carried out this study using immunoblotting to detect the allergens of B. tropicalis. Our results demonstrate that this mite has one major allergen (11–13kDa) and three other important allergens with about 50% binding (64, 36 and 33 kDa). Therefore, B. tropicalis should be regarded as an important source of allergens in the house dust in tropical areas, besides those derived from other mites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1994.tb02743.x
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  • 2
    Electronic Resource
    Electronic Resource
    Mite fauna, Der p I, Der f I and Blomia tropicalis allergen levels in a tropical environment (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fifty dust samples were collected from the mattresses and bedroom floors of 25 subjects with allergic asthma in Cartagena, Colombia, in order to identify house dust mites and quantitate Der p I, Derf I and Blomia tropicalis allergens. The geometric mean of the total mite density per gram of dust was 418 (range, 40–2280). Twenty-two samples (44%) had more than 500 mites and four, less than 100. B. tropicalis and Dermatopha-goides pteronyssinus were found in 96% and 90% of the samples, accounting for 40.1% and 35.7% of the total mites, respectively. Cheyletus malaccensis, Chortoglyphus arcuatus, Pyroglyphus africanus, Orihatids, Grallacheles bakeri. Tarsonemus spp., Suidasia spp., Dermatophagoides farinae and unidentified mites accounted for the rest. The geometric mean of the total mites/gram of dust in mattresses (563.9) was significantly higher than in floor dust (309. 1), P 〈 0.01. Allergen concentrations and mite numbers were analysed by Spearman rank correlations: B. tropicalis mites vs B. tropicalis allergen, r= 0.54, P〈0.001; D. pteronyssinus mites vs Der p I, r= 0.52, P〈 0.001. A negative correlation was obtained between B. tropicalis mites and Der p I. Allergens derived from B. tropicalis and other domestic mite species may play an important role in sensitization and allergic symptoms in Cartagena, Colombia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1993.tb00325.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Sensitization to Chortoglyphus arcuatus and Aleuroglyphus ovatus in Dermatophagoides spp. allergic individuals (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The prevalence of specific IgE to the storage mites Aleuroglyphus ovatus (Ao) and Chortoglyphus arcuatus (Ca) was studied in 77 individuals with allergic asthma and/or chronic allergic rhinitis. All these individuals had a positive skin test (weal ≥ 3 mm) to extracts of Dermatophagoides pteronyssimis (Dp) and/or Dermatophagoides farinac (Df). Sera from 29 non-atopic individuals were used as controls. A RAST was considered positive when a serum bound ≥1% of the total counts added. The prevalence of a positive RAST to Dp was 75.3%, and to at least one of the two storage mites (Ao and Ca), 76.6%. Among patients with a positive RAST to Dp. 79.3% and 75.8% were RAST positive to Ao and Ca, respectively. RAST inhibition studies with a pool of sera from 13 subjects with high RAST binding to all three mites showed significant crossreactivity between Ao and Ca and minimal to moderate crossreactivity between Dp and Ao and Co, This study demonstrates that Sensitization to Ao and Ca is common in individuals with respiratory allergies in Cartagena, Colombia and suggests that Ao, Ca and Dp have unique and common allergenic determinants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1993.tb00306.x
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  • 4
    Electronic Resource
    Electronic Resource
    Allergenic composition of the mite Suidasia medanensis and cross-reactivity with Blomia tropicalis (2005)
    Oxford, UK : Munksgaard International Publishers
    Allergy 60 (2005), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Mites of the genus Suidasia are commonly found in house dust and may play an allergenic role in exposed populations. However, the allergenic potential and clinical impact of this genus has not been well established. The main objective of this project was to evaluate the allergenic role of the mite Suidasia medanensis.Methods:  An extract of S. medanensis was prepared and the allergen composition determined by immunoblot. Specific IgE antibody levels to S. medanensis and Blomia tropicalis were evaluated by radioallergosorbent (RAST) in the sera of 97 allergic asthmatic patients and 50 nonallergic subjects. Cross-reactivity between S. medanensis and the mite species B. tropicalis and Dermatophagoides farinae was investigated by RAST and immunoblot inhibitions.Results:  Seventy-one asthmatic patients sera (73.2%) had positive IgE reactivity to S. medanensis; 14 allergens with molecular weights ranging from 7.5 to 105 kDa were detected. The most frequently detected had molecular weights of 30–31 (54.8%), 24.5 (42%), 21 (38.7%), 47 (35%) and 58 kDa (35.5%). Blomia tropicalis extract inhibited IgE binding to nine of these identified allergens. Four B. tropicalis allergens were inhibited by S. medanensis extract. RAST inhibition results demonstrated a high degree of inhibition by B. tropicalis (87.2%) and D. farinae (90.9%) than by S. medanensis (32%).Conclusions:  Sensitization to S. medanensis is common in asthmatic allergy patients in Cartagena. An important degree of cross-reactivity was established between S. medanensis and B. tropicalis, and D. farinae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.2004.00636.x
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    Paper (German National Licenses)
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