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  • Electronic Resource  (2)
  • 1985-1989  (2)
  • 1900-1904
  • Blutspuren, Antigeneigenschaften  (1)
  • Dibutyryladenosine 3′,5′-cyclic monophosphate  (1)
  • 1
    ISSN: 1437-1596
    Keywords: Bloodstains species identification ; Species identification, immunoassay (ELISA) ; Blutspuren, Antigeneigenschaften ; Speziesidentifikation, Immunoassay (ELISA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Die Antigeneigenschaften von Blutspuren menschlicher und nichtmenschlicher Primaten und anderer Tiere wurden mit Hilfe des Inhibitions-ELISA unter Benutzung käuflich erhältlicher Anti-Human-Albumin-und Anti-Alpha2-makroglobulin-, Fibrinogen-, Transferrin- und IGG-Seren untersucht. Allgemein kann festgestellt werden, daß Blutspuren von Schimpansen die stärksten Kreuzreaktionen mit diesen Antiseren aufwiesen und daß das Ausmaß der Kreuzreaktionen von Blutspuren anderer Tiere deutlich in Verbindung mit dem phylogenetischen Rang, wie folgt, abnahm: agiler Gibbon, Hundsaffen (Japanischer Affe, Mantelpavian), Breitnasenaffen (Nachtaffen und Kapuziner), Halbaffen (Groß-Galago und Katta) und andere Tiere (Ratte, Rind, Schwein, Ziege, Hund, Katze und Huhn). Unter diesen Antiseren zeigten Anti-Human-Alpha2-Makroglobulin-Seren die schwächsten Kreuzreaktionen mit Blutspuren von Schimpansen; Anti-HumanAlbumin folgte hiernach.
    Notes: Summary Antigenic properties of bloodstains of human and non-human primates as well as other animal bloodstains were investigated by the inhibition ELISA using commercially available anti-human albumin (Alb), α2-macroglobulin (α2-M), fibrinogen, transferrin, and immunoglobulin G. In general, chimpanzee bloodstains showed strong cross-reactions with these antisera, and the extent of the cross-reactions of other animal bloodstains decreased largely with the phylogenic order, i.e., agile gibbon (ape), Old World monkeys (Japanese monkey and hamadryas baboon), New World monkeys (night monkey and tufted capuchin monkey), prosimians (grand galago and ring-tailed lemur) and other animals (rat, cattle, swine, goat, dog, cat, and chicken). Among these antisera, anti-human α2-M showed the weakest cross-reaction with chimpanzee bloodstains, and anti-human Alb showed next.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0533
    Keywords: Glutathione S-transferase placental type ; T9 glioma cells ; Dibutyryladenosine 3′,5′-cyclic monophosphate ; Allylisothiocyanate ; Benzylisothiocyanate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of dibutyryladenosine 3′,5′-cyclic monophosphate (dibutyryl cAMP) on the expression of glutathione S-transferase placental type (GST-P) was examined in rat glioma cell line using an immunohistochemical technique. Cultured T9 glioma cells were negative for GST-P activity under normal conditions. However, treatment with 1 mM dibutyryl cAMP produced GST-P expression in about 50% of the cells, as well as some morphological changes. The expression of GST-P was increased with addition of dibutyryl cAMP together with 1 μg/ml allyl isothiocyanate (AITC) or 0.1 μg/ml benzyl isothiocyanate (BITC). With these combinated treatments, almost all cultured cells showed a strong positive reaction for GST-P, although AITC or BITC alone elicited GST-P in only 5% of the cultured cells. The results of the present study indicate that dibutyryl cAMP causes functional as well as morphological differentiation of T9 glioma cells.
    Type of Medium: Electronic Resource
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