ISSN:
1420-9071
Keywords:
Basic protein
;
purification
;
turbidimetry
;
co-sedimentation with tubulin
;
bovine brain
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A 193 kDa protein consisting of 58 kDa subunits, which has pI values of 8.50 and 8.65, was purified from bovine brain cytosol. It formed heavy precipitates with tubulin, and the molar ratio of tubulin dimer to this protein in the precipitate was 3.2. In contrast to microtubules containing ordinary microtubule-associated proteins, these complexes remained stable against cold and 1 mM CaCl2.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01954860
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