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  • Electronic Resource  (6)
  • 1980-1984  (2)
  • 1970-1974  (4)
  • Life and Medical Sciences  (3)
  • Chemistry  (2)
  • Retrograde tracing  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Tectal paths of regenerated optic axons in the goldfish: Evidence from retrograde labelling with horseradish peroxidase (1983)
    Springer
    Experimental brain research 51 (1983), S. 433-442 
    Details
    ISSN: 1432-1106
    Keywords: Optic axons ; Regeneration ; Optictectum ; Retrograde tracing ; Goldfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To compare the distributions of normal and regenerated optic axons in the goldfish tectum, small groups of axons crossing the rostromedial tectum were cut and filled with horseradish peroxidase which subsequently revealed the retinal locations of their somata. In normal fish, the peroxidase-filled ganglion cells were virtually confined to a narrow arc spanning the ventronasal quadrant of the retina. In fish with regenerated visual projections (50–736 days after optic nerve transection, optic nerve crush or deflection of optic axons to the ipsilateral tectum) the filled cells were distributed across the full extent of the retina from centre to periphery and were less rigidly confined within appropriate quadrants. The absence of any detectable arc of filled cells in the ventronasal quadrant after regeneration showed that few, if any, of the regenerated axons followed their original paths across the tectum. Quantitative analysis of local cell distributions indicated that axons were re-routed independently rather than in groups. Nevertheless, axons consistently displayed a crude bias towards appropriate tectal regions, even in ipsilateral tecta where the relative positions of these regions are inverted. These results imply that regenerating optic axons are widely scattered by the effects of surgery. They may subsequently show preferences for appropriate central paths but with a resolution too low to define much more than the orientation of the retino-tectal map. Since there is both anatomical and electrophysiological evidence that regenerated optic terminal arborizations eventually adopt a precise retinotopic arrangement, this arrangement must chiefly reflect ordering mechanisms which act in the final stages of axon growth or synapsis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237880
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A quantitative method of sample loading for field desorption mass spectrometry (1974)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 1 (1974), S. 358-362 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A quantitative method for loading nanogram samples onto field desorption emitter wires is described. Sample consumption is reduced and problems with emitter wettability are overcome by freezing microliter droplets onto the emitters. Using this technique, good spectral data are obtained from 10 to 50 ng of material. Field desorption sensitivity measurements are reported for neomycin B, adenosine and cholesterol. Evaluation of the nonquantitative dipping technique previously employed reveals poor and nonreproducible sample loading efficiency.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200010513
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Field desorption mass spectrometry in structural studies of polyene macrolide antibiotics: Isolation and early identification of a pentaene macrolide antibioticSee Ref. 1.Abbreviation: IPA=2-propanol. (1980)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 7 (1980), S. 93-98 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Isolation of a pentaene macrolide antibiotic (NSC-277813) from Streptomyces griseus (FCRC-21) fermentation broth is described. Using field desorption mass spectrometry and high resolution field desorption mass spectrometry on the intact and derivatized antibiotic and degradation products, the antibiotic was identified as fungichromin. The application of field desorption mass spectrometry in the identification of polyene antibiotics is discussed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200070302
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of culture pH on photo-inhibition of division in Euglena gracilis (1971)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 78 (1971), S. 273-276 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In the dark, growth rates of Euglena gracilis were independent of culture pH between the limits of three to eight. Visible light of moderate intensity inhibited growth rates, with the degree of inhibition being markedly pH dependent. The most severe inhibition was observed at pH 4 to 5, with little or no inhibition at pH 3 or above pH 6.8 at the light intensity used (400 foot-candles).
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040780215
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  • 5
    Electronic Resource
    Electronic Resource
    Factors affecting pH-dependent photo-inhibition of division in Euglena gracilis (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 82 (1973), S. 489-495 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Visible light of moderate intensity (1200 ft-cd) can severely inhibit cell division of a non-photosynthetic mutant of Euglena gracilis when growth is supported by butanol, ethanol, or fumarate as sole carbon source. The degree of inhibition is pH dependent, being greatest at pH 4 to 5. A wide variety of other carbon sources permitted growth in the light without inhibition.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040820317
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  • 6
    Electronic Resource
    Electronic Resource
    Factors affecting division rhythmicity in Euglena gracilis (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 84 (1974), S. 291-299 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In phototrophic culture of Euglena gracilis, good synchrony was found only under rather restricted programs of light-dark cycles, and rather narrow ranges of temperature and light intensity, when cultures were flushed with air fortified with adequate amounts of CO2. When flushed with air alone, CO2 was found to be limiting, and while cell divisions were rhythmic, less than a doubling of cell number occurred in division bursts. With air as gas phase, rhythmic division activity was maintained over wide ranges of temperature, light intensity, and the ratio of light:dark in a given program; all these factors affected the amplitude of the division burst, however.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040840215
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    Paper (German National Licenses)
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