ISSN:
1432-2242
Keywords:
Intergeneric hybrids
;
Embryo cultures
;
Giemsa banding
;
Crossability
;
B chromosomes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Rye (Secale cereale cv. Prolific 2n=14 and 2n =14 + 2B was crossed onto hybrids between barley (Hordeum vulgare 2n = 14) and wheat (Triticum aestivum 2n= 42). Pollinated florets were injected with GA3 to promote fertilization and hybrid embryo development. At 16 days after pollination the watery caryopses were removed, embryos dissected and cultured on a modified B5 medium. Approximately 20% of the cultured embryos produced both roots and coleoptile and developed into viable seedlings. Viable seeds were also obtained at a low frequency from the same cross combinations. The hybrids were wheat-like except for the hairy neck characteristic of rye. There were 35 chromosomes in somatic tissue; 21 wheat, 7 barley and 7 rye. The rye chromosomes were distinguishable by their larger size and terminal C-bands. A lower seed set was obtained using pollen from rye plants with 2n=14 + 2B chromosomes than from plants without B chromosomes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00295452
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