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  • Electronic Resource  (4)
  • 1965-1969  (4)
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  • Electronic Resource  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    Subcellular Preparation of Peripheral Nerve Myelin (1968)
    [s.l.] : Nature Publishing Group
    Nature 220 (1968), S. 171-173 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In discussion at a symposium at Liblice2, Professor E. Gutmann and Dr H. Koenig suggested that we should try to prepare a residue of myelin from dorsal spinal roots by plucking out their axons with forceps after treating these peripheral nerves with a glycine-diethylamine buffer3. This suggestion ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/220171a0
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Reappraisal of osmium tetroxide and OTAN histochemical reactions (1968)
    Springer
    Histochemistry and cell biology 16 (1968), S. 162-166 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Osmium tetroxide and OTAN histochemical reactions have been reappraised following recent publications in this journal by Ellbder and Lojda. Provided that the standard OTAN reaction is used on frozen sections of conventional thickness, unsaturated hydrophilic polar lipids are stained in an orange or red shade while unsaturated hydrophobic non-polar lipids are stained brown-black or black. We have confirmed the anomaly — pointed out by Elleder and Lojda — that the “polar lipid” reaction of the atherosclerotic plaque is essentially extinguised by acetone. Solubility and blockading methods provide circumstantial evidence that Elleder and Lojda's assumed OsO4-protein reactions are due to the lipid moiety of a lipoprotein complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280614
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Osmium tetroxide as a histochemical and histological reagent (1967)
    Springer
    Histochemistry and cell biology 9 (1967), S. 68-77 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary From the evidence discussed it can be concluded that osmium tetroxide (OsO4) would be reduced to black OsO2 (or an equivalent compound) by the ethylene bonds of liquid or solid cis-unsaturated lipids or by the Δ 5-double bond in cholesterol in solid state in tissues. No evidence has been obtained to suggest that OsO4 is either reduced or bound by proteins and polysaccharides in tissue-sections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281808
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cholinesterases in peripheral nervous system. III. Validity of localisation around the node of Ranvier (1968)
    Springer
    Journal of molecular histology 1 (1968), S. 68-77 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis A series of experiments was designed to exclude the possibility that the acetylcholinesterase reaction of the rat's node of Ranvier is artefactual. Copper ions were not bound by perinodal acid mucopolysaccharides (or axons) at pH 6.0, which is the same value as that used in the histochemical cholinesterase techniques. No positive evidence was found for diffusion of acetylcholinesterase out of teased nerve fibres. Likewise, no evidence was obtained that acetylcholinesterase, acetylthiocholine or thiocholine are bound at pH 6.0 by perinodal acid mucopolysaccharides (or axons). It is concluded that local non-specific binding of copper, enzyme, substrate or reaction-product cannot account for the acetylcholinesterase reaction in either the node of Ranvier or the axon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01054295
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    Paper (German National Licenses)
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