ISSN:
1433-8580
Keywords:
Biliary sepsis
;
125I-labeledE. coli
;
Retrograde intrabiliary injection
;
Biliary obstruction
;
Rat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 125I-labeledE. coli was injected into the biliary tree of normal rats and rats with 3 weeks' obstruction of the common bile duct to investigate the liver clearance capacity for bacteria. Bile was collected during 15 min, immediately, 1h, 4h, or 24h after the injection. Tissue specimens from the liver, lungs, spleen and kidneys, and blood and urine specimens were collected simultaneously. In normal rats, 40% of the bacteria was recovered in the bile immediately after the injection, whereas 30% was already trapped in the liver. Incubation of the bacteria in the bile duct for 1h, 4h, and 24h resulted in liver retentions of 43%, 15%, and 4%, respectively. The recovery in the bile was 13% after 1-h incubation, and further prolongation of the incubation did not result in a significant decrease. In contrast to these findings, 70% of the injected bacteria was retained in the biliary tree in rats with chronic biliary obstruction (P 〈 0.05 as compared to normal rats) and only 1% was trapped in the liver (P 〈 0.005) 15 min after injection. One-hour incubation of bacteria in the bile duct decreased the retention in the bile to 30%, but the retention in the liver increased only slightly in these animals. Four and 24h after injection less than 30% of the bacteria was retained in the hepato-biliary system. Most of these animals showed almost no radioactivity exceeding the background count in the blood, urine, spleen, lungs, and kidneys 15 min after injection. It was concluded that the impaired clearance capacity of the liver in chronic biliary obstruction might contribute to the susceptibility of such animals for bacteria introduced in the bile.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01852031
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