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  • Electronic Resource  (4)
  • Compatible solutes  (2)
  • 127Xe-Radionuclide ventriculography  (1)
  • Adenine  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The role of trehalose as a substitute for nitrogen-containing compatible solutes (Ectothiorhodospira halochloris) (1990)
    Springer
    Archives of microbiology 153 (1990), S. 607-613 
    Details
    ISSN: 1432-072X
    Keywords: Anaerobic phototrophic bacteria ; Halophilic eubacteria ; Osmoadaptation ; Betaine Ectoine ; Trehalose ; Compatible solutes ; Ectothiorhodospira halochloris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The halophilic phototrophic bacterium Ectothiorhodospira halochloris is able to synthesize both nitrogen-containing (betaine, ectoine) and nitrogen-free (trehalose) compatible solutes. In the absence of external ammonium and under nitrogen-limited growth conditions ectoine was metabolized and trehalose partly replaced betaine. The cytoplasmic trehalose concentration did not exceeded 0.5 mol/kg water (approx. 30% of total compatible solutes). A decreasing content of betaine in cells growing under nitrogen limitation is a result of decreased biosynthesis. Apparently, the betaine pool cannot be used as a nitrogen source, not even in a situation of total nitrogen depletion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00245273
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Intracellular salt and solute concentrations in Ectothiorhodospira marismortui: glycine betaine and Nα-carbamoyl glutamineamide as osmotic solutes (1991)
    Springer
    Archives of microbiology 156 (1991), S. 350-355 
    Details
    ISSN: 1432-072X
    Keywords: Ectothiorhodospira ; Halophilic ; Compatible solutes ; Glycine betaine ; Nα-carbamoyl glutamineamide ; Sucrose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ectothiorhodospira marismortui, a moderately halophilic purple sulfur bacterium from a hypersaline sulfur spring, contains glycine betaine and Nα-carbamoyl glutamineamide (CGA) as the main intracellular osmotic solutes, with sucrose as a minor component. The concentration of glycine betaine was found to increase with increasing salt concentration of the medium, from 0.47 M to 1.29 M in cells grown from 0.85 to 2.56 M NaCl, while the estimated CGA concentration rose from about 0.2 M to 0.5 M. The concentration of sucrose remained constant at a value of around 0.05 M. Intracellular sodium and potassium concentrations were relatively low (around 0.5 and 0.3 M, respectively, at an external NaCl concentration of 1.8 M). The concentration of the novel compound Nα-carbamoyl glutamineamide was enhanced when l-glutamine was added to the growth medium, suggesting that glutamine served as a precursor for the synthesis of the compound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248709
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Adenine requirement for nodulation of pea by an auxotrophic mutant of Rhizobium leguminosarum (1974)
    Springer
    Archives of microbiology 100 (1974), S. 219-238 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium ; Nodulation ; Symbiosis ; Adenine ; Thiamine ; Auxotroph ; Prototroph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A non-nodulating auxotroph (L4-73) derived from an effective strain (L4) of Rhizobium leguminosarum has a growth requirement for adenine and thiamine. The auxotroph was able to infect the roots of the host plant Pisum sativum L. but formed root nodules (ineffective in nitrogen fixation) only when adenine and, to a lesser extent, thiamine were added to the plant substrate. Nodules formed in the presence of adenine were structurally abnormal, containing small cells in which infection threads appeared to have aborted. In the presence of thiamine the auxotroph produced a smaller number of nodules which were slightly more developed and were able to reduce trace amounts of acetylene to ethylene. The adenine effect predominated when both growth factors were added together or separately in different sequences. Adenine amendment was required during the first 6 days following inoculation to achieve the maximum number of nodules. The block in symbiosis could not be fully overcome by sequential addition or removal from the root medium of either compound or by addition of some other adeninecontaining compounds. Partial prototroph revertants requiring adenine but not thiamine induced a nodulation response similar to that of the original auxotroph in the presence of thiamine; partial prototrophs requiring thiamine only were almost fully effective. Bioassay of pea root tissue indicated the presence of significant amounts of both adenine and thiamine or related substances in the roots. The auxotroph was able to compete with the parent strain L4 in nodulation on roots of pea only in the presence of exogenous adenine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446319
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A new method of imaging the right ventricle using peripheral vein infusion of xenon 127 (1987)
    Springer
    European journal of nuclear medicine 12 (1987), S. 617-619 
    Details
    ISSN: 1619-7089
    Keywords: 127Xe-Radionuclide ventriculography ; Right ventricular function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report the development of a new method for obtaining right ventriculograms using the peripheral intravenous infusion of a new tracer, 127Xe dissolved in saline. This tracer has a half life of 36 days, emits 172 keV and 203 keV photons, and is completely cleared by the lungs during pulmonary transit. The right ventricle can therefore be imaged free from interfering activity in the systemic circulation. The technique was used in 11 normal subjects and the results compared with those obtained using first pass and gated equilibrium blood pool angiography with 99mTc. Excellent images of the right ventricle were obtained and the tricuspid and pulmonary valve planes could be easily identified. This imaging technique has significant advantages over existing methods for the noninvasive assessment of right ventricular function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00284538
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    Paper (German National Licenses)
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