Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Electronic Resource  (3)
  • Microspore protoplasts  (2)
  • 25.40.Ve  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A selection method for the synthesis of triploid hybrids by fusion of microspore protoplasts (n) with somatic cell protoplasts (2n) (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 237-243 
    Details
    ISSN: 1432-2242
    Keywords: Microspore protoplasts ; Somatic protoplasts ; Fusions ; Triploids ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Microspore protoplasts (n) isolated at the tetrad stage from plants of Nicotiana tabacum Km+ (2n=4x=48) were fused with somatic cell protoplasts (2n) of WT N. rustica (2n=4x=48) to produce triploid plants. A total of 21.2×106 microspore protoplasts were fused with 11.2×106 somatic cell protoplasts using the high pH/Ca+ + method. Microspore protoplasts did not divide and WT N. rustica protoplasts stopped dividing when the protoplast-derived colonies were transferred to a selection medium containing kanamycin. A total of 104 actively growing green colonies were recovered on the selection medium. Ninety-six of these colonies were tested for their hybrid nature by PAGE of peroxidases and were found to contain bands characteristic of both parents. Hybrid nature of the plants regenerated from some of the selected colonies was confirmed by IEF of leaf esterases, by NPT II activity assay and by hybridizing total DNAs restricted with EcoR I to a cloned 18s rDNA fragment. Root tip squashes of six of the hybrid plants revealed chromosome numbers ranging from 58–72. From chromosomal and biochemical analysis, it can be concluded that the procedure of fusing microspore protoplasts (n) of species A carrying a dominant selection marker with WT somatic cell protoplasts (2n) of species B can be a convenient selection method for the synthesis of triploid plants. The significance of triploids lies in their subsequent use for transferring alien chromosomes and genes of species A to species B.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257851
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Transmission of organelles in triploid hybrids produced by gametosomatic fusions of two Nicotiana species (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 547-552 
    Details
    ISSN: 1432-2242
    Keywords: Microspore protoplasts ; Protoplast fusion ; Gametosomatic hybrids ; Organelle transmission ; Nicotiana species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gametosomatic hybrids produced by the fusion of microspore protoplasts of Nicotiana tabacum Km+Sr+ with somatic cell protoplasts of N. rustica were analysed for their organelle composition. For the analysis of mitochondrial (mt)DNA, species-specific patterns were generated by Southern hybridization of restriction endonuclease digests of total DNA and mtDNA with four DNA probes of mitochondrial origin: cytochrome oxidase subunit I, cytochrome oxidase subunit II, 26s rDNA and 5s-18s rDNA. Of the 22 hybrids analyzed, some had parental-type pattern for some probes and novel-type for the others, indicating interaction between mtDNA of the two parent species. For chloroplast (cp)DNA analysis, species-specific patterns were generated by Southern hybridization of restriction endonuclease digests of total DNA with large subunits of ribulose bisphosphate carboxylase and cpDNA as probes. All the hybrids had N. rustica-specific patterns. Hybrids were not resistant to streptomycin, a trait encoded by the chloroplast genome of N. tabacum. In gametosomatic fusions of the two Nicotiana species, mitochondria but not the chloroplasts are transmitted from the parent contributing microspore protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290841
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Intermittency and fractals in nuclear collisions at 1.52 A GeV (1993)
    Springer
    The European physical journal 345 (1993), S. 305-309 
    Details
    ISSN: 1434-601X
    Keywords: 25.70.Np ; 13.85.Hd ; 25.40.Ve ; 96.40.Mn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The techniques of scaled factorial moments and multifractals have been employed to study the dynamical fluctuations of the produced singly charged particles in collisions of84Kr ions at 1.52 A GeV in nuclear emulsion. The power law behaviors are observed in the data. The generalized dimensions are determined by using the methods of intermittency and multifractals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280838
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...