ISSN:
1432-072X
Keywords:
Rhizobium leguminosarum
;
Alkaline phosphatase
;
Phosphomonoesterase
;
Mg2+, Zn2+-enzyme, K+ activation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The alkaline phosphatase (EC 3.1.3.1.) from Rhizobium leguminosarum WU235 has been purified. The enzyme is a non-specific phosphomonoesterase, has a molecular weight of 78,500 and a sub-unit molecular weight of 39,400. Magnesium and zinc ions are implicated in the structure of the enzyme; atomic absorption analysis gave 1.9 g-atoms Mg2+ and 1.9–5.1 g-atoms Zn2+ per mole of enzyme. In addition high concentrations of Mg2+ markedly stimulate the enzyme. The phosphatase is inhibited by Li+ and Na+ and stimulated by K+, Rb+ and Cs+, which suggests that the enzyme is K+ activated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00409928
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