ISSN:
1612-1112
Keywords:
Reversed-Phase Chromatography
;
Fluorescence detection
;
Ampicillin
;
Plasma
;
Broncho-alveolar lavage fluid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A high performance liquid chromatographic analysis of ampicillin in plasma and broncho-alveolar lavage fluid, using amoxicillin as internal standard, is described. The samples are deproteinated with trichloroacetic acid. For both ampicillin and amoxicillin, a fluorescent degradation product is formed in the presence of mercury(II)-chloride, after alkaline degradation and acid hydrolysis. Chromatography is done on a Spherisorb 5 ODS column using methanol-water (60∶40) with PicRB-7 (1-heptane sulphonic acid) as mobile phase and fluorescence detection (Ext 345 nm, Em 425 nm). Calibration curves for ampicillin were linear over the concentration range 1–100 μg/ml in 100 μl plasma samples and over the concentration range 20–2000 ng/ml in 0.5 ml samples of broncho-alveolar lavage fluid. Quantitative analyses with relative standard deviations of less than 10% are achieved.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02260455
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