ISSN:
1432-1432
Keywords:
Gene structure
;
Heat shock
;
hsp70
;
Antiparallel ORFs
;
Drosophila
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A clone isolated from a Drosophila auraria heat-shock cDNA library presents two long, antiparallel, coupled (LAC) open reading frames (ORFs). One strand ORF is 1,929 nucleotides long and exhibits great identity (87.5% at the nucleotide level and 94% at the amino acid level) with the hsp70 gene copies of D. melanogaster, while the second strand ORF, in antiparallel in-frame register arrangement, is 1,839 nucleotides long and exhibits 32% identity with a putative, recently identified, NAD+-dependent glutamate dehydrogenase (NAD+-GDH). The overlap of the two ORFs is 1,824 nucleotides long. Computational analysis shows that this LAC ORF arrangement is conserved in other hsp70 loci in a wide range of organisms, raising questions about possible evolutionary benefits of such a peculiar genomic organization.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00160312
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