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  • 1
    Electronic Resource
    Electronic Resource
    The β-tubulin gene family evolution in the Drosophila montium subgroup of the melanogaster species group (1995)
    Springer
    Journal of molecular evolution 41 (1995), S. 293-298 
    Details
    ISSN: 1432-1432
    Keywords: β-tubulin ; Evolution ; Gene cluster ; Gene dispersion ; Drosophila montium subgroup
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The β1-, β2-, and β3-tubulin genes have been mapped by in situ hybridization on the polytene chromosomes of 11 selected species (15 strains) belonging to the Drosophila montium subgroup. Although the hybridization pattern among the strains of the same species does not differ, this pattern is significantly different among the species. The β-tubulin genes in the montium subgroup seem to be organized in a cluster, or in a semi-cluster, or are completely dispersed. The clustered arrangement is found in the North-Oriental sibling species D. auraria, D. triauraria, and D. quadraria. The semi-clustered arrangement, wherein the β1 and β2 genes are located at the same locus while β3 is at a different one, appears in the South-Oriental species D. bicomuta, D. serrata, and D. birchii, as well as in the Afrotropical species D. diplacantha and D. seguyi. The complete separation of the genes is observed in the Indian species D. kikkawai and D. jambulina and in the Afrotropical species D. vulcana. Based on the above results, a possible mode of evolution of the β-tubulin genes in the montium subgroup is attempted. In addition, phylogenetic relationships among the montium species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186541
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  • 2
    Electronic Resource
    Electronic Resource
    A Drosophila hsp70 gene contains long, antiparallel, coupled open reading frames (LAC ORFs) conserved in homologous loci (1995)
    Springer
    Journal of molecular evolution 41 (1995), S. 414-420 
    Details
    ISSN: 1432-1432
    Keywords: Gene structure ; Heat shock ; hsp70 ; Antiparallel ORFs ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A clone isolated from a Drosophila auraria heat-shock cDNA library presents two long, antiparallel, coupled (LAC) open reading frames (ORFs). One strand ORF is 1,929 nucleotides long and exhibits great identity (87.5% at the nucleotide level and 94% at the amino acid level) with the hsp70 gene copies of D. melanogaster, while the second strand ORF, in antiparallel in-frame register arrangement, is 1,839 nucleotides long and exhibits 32% identity with a putative, recently identified, NAD+-dependent glutamate dehydrogenase (NAD+-GDH). The overlap of the two ORFs is 1,824 nucleotides long. Computational analysis shows that this LAC ORF arrangement is conserved in other hsp70 loci in a wide range of organisms, raising questions about possible evolutionary benefits of such a peculiar genomic organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00160312
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  • 3
    Electronic Resource
    Electronic Resource
    The Heat-Shock Gene hsp83 of Drosophila auraria: Genomic Organization, Nucleotide Sequence, and Long Antiparallel Coupled ORFs (LAC ORFs) (1998)
    Springer
    Journal of molecular evolution 46 (1998), S. 334-343 
    Details
    ISSN: 1432-1432
    Keywords: Key words:Drosophila auraria—montium subgroup —hsp83— Heat shock (hsp) genes — Overlapping ORFs — LAC ORFs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The genomic organization of the hsp83 gene of Drosophila auraria, a far-eastern endemic species belonging to the montium subgroup of the melanogaster species group, is presented here. Based on in situ hybridization on polytene chromosomes, cDNA and genomic clone mapping, nucleotide sequencing, and genomic Southern analysis, hsp83 is shown to be present as a single-copy gene at locus 64B on the 3L chromosome arm in D. auraria. This gene is organized into two exons separated by a 929-bp intron. The first exon represents the mRNA leader sequence and is not translated, while the coding region, having a length of 2,151 bp, is solely included in the second exon. Nucleotide sequence comparisons of D. auraria hsp83 with homologous sequences from other organisms show high conservation of the coding region (88–92% identity) in the genus Drosophila, in addition to the conserved genomic organization of two-exons–one-intron, of comparable size and arrangement. A phylogenetic tree based on the protein sequences of homologous genes from representative organisms is in accord with the accredited phylogenetic position of D. auraria. In the hsp83 gene region, a second case of long antiparallel coupled open reading frames (LAC ORFs) for this species was found. The antiparallel to the hsp83 gene ORF is 1,554 bases long, while the two ORFs overlap has a size of 1,548 bp. The anti-hsp83 ORF does not show significant homology to any known gene sequences. In addition, no similar LAC ORF structures were found in homologous gene regions of other organisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006310
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  • 4
    Electronic Resource
    Electronic Resource
    The β-tubulin gene family evolution in theDrosophila montium subgroup of themelanogaster species group (1995)
    Springer
    Journal of molecular evolution 41 (1995), S. 293-298 
    Details
    ISSN: 1432-1432
    Keywords: β-tubulin ; Evolution ; Gene cluster ; Gene dispersion ; Drosophila montium subgroup
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The β1-, β2-, and β3-tubulin genes have been mapped by in situ hybridization on the polytene chromosomes of 11 selected species (15 strains) belonging to theDrosophila montium subgroup. Although the hybridization pattern among the strains of the same species does not differ, this pattern is significantly different among the species. The β-tubulin genes in themontium subgroup seem to be organized in a cluster, or in a semi-cluster, or are completely dispersed. The clustered arrangement is found in the North-Oriental sibling speciesD. auraria, D. triauraria, andD. quadraria. The semi-clustered arrangement, wherein the β1 and β2 genes are located at the same locus while β3 is at a different one, appears in the South-Oriental speciesD. bicomuta, D. serrata, andD. birchii, as well as in the Afrotropical speciesD. diplacantha andD. seguyi. The complete separation of the genes is observed in the Indian speciesD. kikkawai andD. jambulina and in the Afrotropical speciesD. vulcana. Based on the above results, a possible mode of evolution of the β-tubulin genes in the montium subgroup is attempted. In addition, phylogenetic relationships among themontium species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01215176
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  • 5
    Electronic Resource
    Electronic Resource
    The heat shock genes in the Drosophila montium subgroup: chromosomal localization and evolutionary implications (1996)
    Springer
    Chromosoma 105 (1996), S. 104-110 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The hsp70, hsp83, hsrω, and the small heat shock protein genes were mapped on the polytene chromosomes of six species, representative of the geographical distribution of the Drosophila montium subgroup of the melanogaster species group. In addition, based on hybridization conditions, the putative locus of the hsp68 gene is given. In contrast to the situation in the melanogaster subgroup species, the hsp70 locus is single in the montium species. The hsp83, hsrωand the small hsp loci are also single in the montium genomes studied here, a common feature of all Drosophila species. Among the hsp genes studied, the small hsp genes and the hsrω-homologous sequences exhibit a higher degree of divergence between the melanogaster and the montium subgroups. Our results support the idea that the montium subgroup species has a genome organization closer to that of the common ancestor compared with the melanogaster subgroup species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050165
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  • 6
    Electronic Resource
    Electronic Resource
    The hsp70 locus of Drosophila auraria (montium subgroup) is single and contains copies in a conserved arrangement (1998)
    Springer
    Chromosoma 107 (1998), S. 577-586 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract.  The restriction endonuclease pattern of a number of hsp70-homologous clones isolated from a library of heat shock cDNA from Drosophila auraria, a species belonging to the montium subgroup of the melanogaster species group, reveals two types of clones, A and B, differing in a single restriction site. Both types, as well as hsp70-specific probes derived from both hsp70 loci of Drosophila melanogaster, hybridize in situ with a single band at region 32 A of the 2L polytene arm, indicating a clustered organization of the hsp70 gene copies in D. auraria. The longest type B clone was sequenced and it was found that one strand contains an open reading frame (ORF) exhibiting great identity with a previously described hsp70 gene of D. auraria (now denoted as type A) and with its counterparts of D. melanogaster, while its second strand, unlike the type A clone, does not contain a long antiparallel coupled ORF (LAC ORF) because of a base substitution resulting in a premature stop codon. After additional data had been derived from isolation and characterization of hsp70-homologous genomic clones, together with Southern analysis of genomic DNA, we found that two hsp70 gene copies are present at the above locus of D. auraria with an inverted tandem repeat organization, while the presence of a third hsp70 gene is not clearly evident. The above results are compared with those observed at the homologous loci of some melanogaster subgroup species (D. melanogaster and its sibling species), in which, however, the hsp70 locus is duplicated, and with the more distantly related Dipteran Anopheles albimanus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050344
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  • 7
    Electronic Resource
    Electronic Resource
    The glutamate dehydrogenase, E74 and putative actin gene loci in the Drosophila montium subgroup (1997)
    Springer
    Chromosoma 106 (1997), S. 20-28 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. DNA-specific sequences from an enzyme-coding gene (glutamate dehydrogenase, gdh), a regulatory protein-coding gene (E74) and genes of the actin family were mapped by in situ hybridization on the polytene chromosomes of six species representative of the geographical distribution of the Drosophila montium subgroup of the melanogaster species group. In all species studied, one hybridization signal was detected for the gdh and E74 genes, and seven signals for the actin genes. The distribution of the actin-related loci in five montium species is similar to that of the other Drosophila species studied so far, although they present an extra signal. This distribution differs in the sixth montium species studied, D. kikkawai. Taking into account the present results, as well as previous data obtained mainly by in situ hybridizations, homologies among the polytene chromosomes of the montium subgroup species, as well as between these species and D. melanogaster, were also established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050220
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  • 8
    Electronic Resource
    Electronic Resource
    Balbiani rings and puffs of the polytene chromosomes of Drosophila auraria (1984)
    Springer
    Chromosoma 89 (1984), S. 96-106 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Drosophila auraria and its sibling species, D. biauraria, D. triauraria, and D. quadraria are unique among Drosophila species in that their salivary gland chromosomes exhibit Balbiani rings. In this report we present a cytological map of D. auraria and information on the developmental profiles of its puffs and Balbiani rings. Information is presented on the existence of tandem inverted duplications involving the Balbiani ring regions and other regions of the chromosomes, and data are given concerning the puffing patterns of the duplicated bands. Possible homologies between puffs of D. melanogaster and D. auraria and certain differences between the two species in the developmental sequences of the active loci are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292892
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  • 9
    Electronic Resource
    Electronic Resource
    Theβ−tubulin genes ofDrosophila auraria are arranged in a cluster (1994)
    Springer
    Current genetics 25 (1994), S. 84-87 
    Details
    ISSN: 1432-0983
    Keywords: β-tubulin genes ; Gene-cluster ; Drosophila auraria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When the β1-, β2- and β3-tubulin-specific DNAs fromDrosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes ofDrosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were isolated from a λEMBL 3 genomic library ofD. auraria, and they all contain β-tubulin-specific sequences based on hybridization and partial-sequencing experiments of subcloned fragments. These clones hybridize in situ to the same polytene chromosome band in region 32C and they represent an approximately 35-kb fragment of genomic DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00712973
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