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  • Electronic Resource  (3)
  • Assert  (1)
  • Brassica  (1)
  • Embryogenesis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 15 (1996), S. 396-400 
    ISSN: 1432-203X
    Keywords: Brassica campestris ssp. parachinensis ; Microspore ; Embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microspores of several genotypes of Brassica campestris ssp. parachinensis have been cultured in vitro and induced to undergo embryogenesis and plant formation. Conditions favourable for embryogenesis in this species include a bud size of 2–2.9 mm, NLN-13 culture medium (Nitsch and Nitsch 1967; Lichter 1981, 1982; Swanson 1990), and an induction through exposure to 32°C for a period of 48 h. Longer periods of an elevated temperature for induction of embryogenesis resulted in embryo abortion at early developmental stages. With the protocol developed here, microspores of 60–80% of donor plants could be induced to produce embryos, although embryo yields were low, i.e. 2–5 embryos per 10 buds. Some genotypes responded to culture conditions with high numbers of embryo formation (100–150 embryos per 10 buds) but most of these subsequently failed to mature. The pattern of cell division and morphological changes of the microspores in culture were studied using various microscopic techniques.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Microspores ; Haploid ; Pursuit ; Assert ; Tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In vitro microspore mutagenesis and selection was used to produce five fertile double-haploid imidazolinone-tolerant canola plants. The S2 plants of three of the mutants were resistant to at least the field-recommended levels of Assert and Pursuit. One mutant was tolerant to between five and ten times the field-recommended rates of Pursuit and Scepter. Two semi-dominant mutants, representing two unlinked genes, were combined to produce an F1 hybrid which was superior in imidazolinone tolerance to either of the heterozygous mutants alone. Evaluation of the mutants under field conditions indicated that this hybrid and the original homozygous mutants could tolerate at least two times the field-recommended rates of Assert. The field results indicated the mutants were unaffected in seed yield, maturity, quality and disease tolerance. These genes represent a potentially valuable new herbicide resistance system for canola, which has little effect on yield, quality or maturity. The mutants could be used to provide tolerance to several imidazolinones including Scepter, Pursuit and Assert.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 218 (1989), S. 419-422 
    ISSN: 1617-4623
    Keywords: Brassica ; Mitochondrial plasmids ; Maternal/paternal inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 11.3 kb mitochondrial (mt) plasmid was restored to rapeseed (Brassica napus) plants cured of the plasmid (by in vitro culture) by crossing to plasmid-containing males, but not by grafting plasmidless shoots onto plants containing the plasmid. Plasmid restoration is not associated with alterations in mt DNA restriction patterns nor is it likely the result of excision of plasmid sequences from the mt genome. Restoration of the mitochondrially-associated plasmid is probably the result of transmission of cytoplasm from the male parent through the pollen to the egg cell in the female. Pollen transfer of the plasmid also occurred in other crosses regardless of cytoplasmic or nuclear background and at an average rate of 50%. These experiments demonstrate that a cytoplasmic genetic element can be non-maternally inherited in Brassica and suggest that the mitochondria with which this element is associated are transmitted to the egg cell during fertilization.
    Type of Medium: Electronic Resource
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