ISSN:
1573-5028
Keywords:
embryo development
;
gene regulation
;
storage proteins
;
post-transcriptional regulation
;
12S globulin
;
Avena sativa L.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and in situ hybridization. However, immunological tissue printing detected the oat protein exclusively in the tobacco endosperm, suggesting that extensive post-transcriptional regulatory processes influence the expression of the monocot transgene in the dicot host.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00039532
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