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  • 1
    Electronic Resource
    Electronic Resource
    High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L.
    Amsterdam : Elsevier
    Plant Science 74 (1991), S. 235-242 
    Details
    ISSN: 0168-9452
    Keywords: Brassica oleracea ; embryogenesis ; haploid ; microspore culture
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(91)90051-9
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Intergeneric (intersubtribe) hybridization between Moricandia arvensis and Brassica A and B genome species by ovary culture (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 38-42 
    Details
    ISSN: 1432-2242
    Keywords: Brassica campestris ; B. nigra ; Intergeneric hybridization ; Moricandia arvensis ; Ovary culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids between Moricandia arvensis (C3–C4 intermediate species) and Brassica A and B genome species (B. campestris and B. nigra) were produced via ovary culture. When M. arvensis was used as a female parent, the hybrid embryo yield (0.25–0.45 embryo per pollination) was similar between two genomes, regardless of the male parent. The reciprocal hybrid using B. campestris as a female was also obtained, although yield of embryo was lower (0.02 embryo per pollination). On the other hand, no hybrids were obtained without the in vitro technique. As most hybrid embryos could not develop normal shoots, plants were regenerated by inducing shoots on the cultured hypocotyl. The hybrid nature of the regenerated plant was confirmed morphologically and cytogenetically. A certain amount of bivalents (2.52-2.71) in the hybrids indicated the existence of partial chromosome homology between two genera. The present results indicate that ovary culture is an effective technique for overcoming the crossing barrier between M. arvensis and Brassica cultivated species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224013
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L. (1991)
    Springer
    Euphytica 58 (1991), S. 51-55 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; donor plant age ; embryogenesis ; haploid ; microspore culture ; microspore stage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effect of age of donor plants and age of inflorescence on embryogenesis in microspore culture of B. napus was examined. Microspores isolated from buds of older plants had a higher embryo yield than those of younger ones. The effect of the age of inflorescence showed a different pattern. In older plants, a higher embryogenesis response was observed in microspores isolated from buds of new inflorescences, while in young plants, microspores isolated from buds of old inflorescences showed high embryo yield. These different responses were considered to be attributable to a difference in the developmental stage of pollen at the time of microspore isolation. Our results indicated that microspores collected from older inflorescences and older plants have sufficient embryogenic potential when the optimum developmental stage of pollen was used. Frequency of embryo to plant conversion was influenced by the size of embryos subcultured, but not by donor plant age or the age of the inflorescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035339
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Dry artificial seeds and desiccation tolerance induction in microspore-derived embryos of broccoli (1993)
    Springer
    Plant cell, tissue and organ culture 35 (1993), S. 121-129 
    Details
    ISSN: 1573-5044
    Keywords: abscisic acid ; Brassica oleracea ; embryogenesis ; microspore culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Desiccation tolerance of broccoli microspore-derived embryos was induced by exogenous application of abscisic acid (ABA). Embryos, which were desiccated to about 10% water content, were estimated for viability after rehydration. Survival was dependent on the ABA concentration and the development stage of embryo, but not on the length of exposure period to ABA or genotype. Cotyledonary stage embryos acquired the highest desiccation tolerance when treated with 1×10-4M ABA. Under this condition, on average 27–48% of the desiccated embryos could convert into plants. Embryos treated with 1×10-6M ABA or no ABA or earlier development-staged embryos, such as globular and heart stages, lost viability after desiccation. A one day exposure to ABA had the similar effect on the induction of desiccation tolerance as a 7-day treatment. The dried embryos maintained their ability of plant conversion after three months of storage under room conditions. The plants derived from the desiccated embryos were not different in the morphology or ploidy level from those from non-desiccated ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032961
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    Paper (German National Licenses)
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